催乳素(PRL)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Prolactin (PRL)
LTH; Luteotropic Hormone
- 编号CEA846Ga
- 物种Chicken (Gallus，鸡) 相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长2h
- 检测范围2.47-200ng/mL
- 灵敏度最小可检测剂量小于等于0.89ng/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2873 ¥ 4104 ¥ 18468 ¥ 34884 ¥ 287280
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测催乳素(PRL)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的催乳素(PRL)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 88-104 | 96 |
| EDTA plasma(n=5) | 98-105 | 102 |
| heparin plasma(n=5) | 82-97 | 89 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的催乳素(PRL)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中催乳素(PRL)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-96% | 90-98% | 82-97% | 96-103% |
| EDTA plasma(n=5) | 91-101% | 94-105% | 78-104% | 86-101% |
| heparin plasma(n=5) | 81-102% | 88-95% | 91-98% | 93-101% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）50µL，
加入50µL检测液A(临用前配制);
37°C温育1小时。
3. 洗板3次；
4. 加检测溶液B100µL，37°C孵育30分钟；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分钟；
7. 加终止液50µL，立即450nm读数。
实验原理
本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将催乳素(PRL)单克隆抗体包被微孔板，制成固相载体，往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本)，待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物，然后加入HRP标记的亲和素，经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。待测标本浓度越高，标记抗原和抗体的结合就越受到抑制，显色愈浅。显色的深浅与酶量呈正相关，而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度（O.D.值），计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Chicken (Gallus，鸡) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA846Ga01 | 催乳素(PRL)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA846Ga01 | 催乳素(PRL)多克隆抗体 | WB; IHC; ICC; IP. |
| CEA846Ga | 催乳素(PRL)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA846Ga | 催乳素(PRL)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| The Journal of Biological Chemistry | In Vivo Evidence for Epidermal Growth Factor Receptor (EGFR)-mediated Release of Prolactin from the Pituitary Gland [Jcb: 39297] |
| Journal of Clinical Neuroscience | TLR9 expression is associated with prognosis in patients with glioblastoma multiforme [ScienceDirect: S0967586811003572] |
| Sensors and Actuators B: Chemical | Determination of prolactin hormone in serum and urine using an electrochemical immunosensor based on poly(pyrrolepropionic acid)/carbon nanotubes hybrid modified electrodes [Sciencedirect:S0925400514000720] |
| Veterinary Word | Effect of extended photoperiod during winter on growth and onset of puberty in Murrah buffalo heifers [Pubmed:27051212] |
| Journal of Dairy Science | Effect of thermal stress on physiological, hormonal and haematological parameters in Tharparkar and Karan Fries calves [publication:306322503] |
| Stem Cell Research & Therapy | Differentiation of human umbilical cord Wharton’s jelly-derived mesenchymal stem cells into endometrial cells [pubmed:29096715] |
| Stem Cell Research & Therapy | The role of mesenchymal stem cells in chemotherapy-induced gonadotoxicity [Pubmed:30021657] |
| Frontiers in Molecular Neuroscience | Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice [Pubmed:29403355] |
| Toxicology and Applied Pharmacology | The antipsychotics sulpiride induces fatty liver in rats via phosphorylation of insulin receptor substrate-1 at Serine 307-mediated adipose tissue insulin resistance [Pubmed:29551354] |
| Journal of Cellular Biochemistry | The prolactin‐release inhibitor paeoniflorin suppresses proliferation and induces apoptosis in prolactinoma cells via the mitochondria‐dependent pathway [Pubmed:29388711] |
| Journal of Cellular and Molecular Medicine | Metformin inhibits growth and prolactin secretion of pituitary prolactinoma cells and xenografts [Pubmed: 30334324] |
| BMC Veterinary Research | Hormonal and metabolic indicators before and after farrowing in sows affected with postpartum dysgalactia syndrome [Pubmed: 30404636] |
| International Journal of Basic and Clinical Endocrinology | Expression of prolactin receptors in the duodenum, kidneys and skeletal system during physiological and sulpiride-induced hyperprolactinaemia [Pubmed: 30143940] |
| MORPHOLOGY AND PATHOMORPHOLOGY | Morphological and Biochemical Characteristics of Prostate Hyperplasia during Sulpiride Treatment [Pubmed: 32152847] |
| JOURNAL OF DAIRY SCIENCE | Effect of heat stress during the early and late dry period on mammary gland development of Holstein dairy cattle [Pubmed: 32684470] |
| BioMed Research International | Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress [Pubmed: 32685488] |
| Vet Microbiol | Prolactin affects the disappearance of ALV-J viremia in vivo and inhibits viral infection [34391195] |
| Antioxidants (Basel) | Effects of a Nanoencapsulated Moringa Leaf Ethanolic Extract on the Physiology, Metabolism and Reproductive Performance of Rabbit Does during Summer [34439574] |
| Nutrients | Yogurt Enriched with Inulin Ameliorated Reproductive Functions and Regulated Gut Microbiota in Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome Mice [Pubmed:35057459] |
