氧化低密度脂蛋白(OxLDL)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Oxidized Low Density Lipoprotein (OxLDL)
OL
- 编号SEA527Rb
- 物种Oryctolagus cuniculus (Rabbit，兔) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围15.6-1,000pg/mL
- 灵敏度最小可检测剂量小于等于6.9pg/mL.
- 样本类型Serum, plasma and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2873 ¥ 4104 ¥ 18468 ¥ 34884 ¥ 287280
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测氧化低密度脂蛋白(OxLDL)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的氧化低密度脂蛋白(OxLDL)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 86-101 | 97 |
| EDTA plasma(n=5) | 81-95 | 85 |
| heparin plasma(n=5) | 89-99 | 95 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的氧化低密度脂蛋白(OxLDL)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中氧化低密度脂蛋白(OxLDL)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-95% | 86-95% | 82-96% | 96-103% |
| EDTA plasma(n=5) | 92-105% | 97-104% | 97-104% | 83-101% |
| heparin plasma(n=5) | 83-91% | 82-89% | 79-95% | 94-102% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将氧化低密度脂蛋白(OxLDL)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的氧化低密度脂蛋白(OxLDL)与连接于固相载体上的抗体结合，然后加入生物素化的氧化低密度脂蛋白(OxLDL)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的氧化低密度脂蛋白(OxLDL)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
相关产品
| 编号 | 适用物种：Oryctolagus cuniculus (Rabbit，兔) | 应用(仅供研究使用，不用于临床诊断！) |
| SEA527Rb | 氧化低密度脂蛋白(OxLDL)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA527Rb | 氧化低密度脂蛋白(OxLDL)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA527Rb | 氧化低密度脂蛋白(OxLDL)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Journal of Ethnopharmacology | Total panax notoginsenosides prevent atherosclerosis in apolipoprotein E-knockout mice: Role of downregulation of CD40 and MMP-9 expression [ScienceDirect: S0378874109005091] |
| Autonomic Neuroscience | Chemical sympathectomy induces arterial accumulation of native and oxidized LDL in hypercholesterolemic rats [ScienceDirect: S1566070211004152] |
| Cell Biochemistry and Biophysics | Plasma Paraoxonase-1, Oxidized Low-Density Lipoprotein and Lipid Peroxidation Levels in Gout Patients [SpringerLink: u466114p03434m6t] |
| Cardiovascular Research | Endothelial-specific Nox2 overexpression increases vascular superoxide and macrophage recruitment in ApoE-/- mice [PubMed: PMC3307381] |
| Atherosclerosis | 10-Dehydrogingerdione raises HDL-cholesterol through a CETP inhibition and wards off oxidation and inflammation in dyslipidemic rabbits. [Pubmed: 24267247] |
| PloS one | Atheroprotective effect of oleoylethanolamide (OEA) targeting oxidized LDL. [NCBI: PMC3896367] |
| Atherosclerosis.? | Insulin-like growth factor I reduces lipid oxidation and foam cell formation via downregulation of 12/15-lipoxygenase [Pubmed:25549319] |
| J Surg Arts | THE FUNCTION OF SIALIC ACID AS A RADICAL SCAVENGER IN EXPERIMENTAL HYPOTHYROIDISM WITH AND WITHOUT HYPERLIPIDEMIA. [Cerrahisanatlardergisi:Source] |
| PLoS One. | Atheroprotective effect of oleoylethanolamide (OEA) targeting oxidized LDL [Pubmed:24465540] |
| Saudi Med J | Increased systemic low-grade inflammation in high altitude native rats mediated by adrenergic receptors [Pubmed:24888651] |
| Vascular Medicine | Anti‐Inflammatory Immune Skewing Is Atheroprotective: Apoe−/−FcγRIIb−/− Mice Develop Fibrous Carotid Plaques [Pubmed:25516435] |
| Journal of Functional Foods | Oral administration of the β-glucan produced by Aureobasidium pullulans ameliorates development of atherosclerosis in apolipoprotein E deficient mice [Science: Article] |
| Acta Pharmacologica Sinica | Sirt1 is involved in decreased bone formation in aged apolipoprotein E-deficient mice [PubMed: 26592520] |
| Cancer Science | Suppressive effects of the NADPH oxidase inhibitor apocynin on intestinal tumorigenesis in obese KK‐Ay and Apc mutant Min mice [PubMed: 26310859] |
| BMJ Open Sport Exerc Med | Athletes with higher VO2max present reduced oxLDL after a marathon race [Content: 1] |
| Biochim Biophys Acta | PPARβ/δ ameliorates fructose-induced insulin resistance in adipocytes by preventing Nrf2 activation [PubMed: 25728706] |
| PLoS One | Ezetimibe Attenuates Atherosclerosis Associated with Lipid Reduction and Inflammation Inhibition [PubMed: 26555472] |
| PLoS One | Pulsatile Hyperglycaemia Induces Vascular Oxidative Stress and GLUT 1 Expression More Potently than Sustained Hyperglycaemia in Rats on High Fat Diet [Pubmed:26790104] |
| Diabetology & Metabolic Syndrome | Oxidative DNA damage and oxidized low density lipoprotein in Type II diabetes mellitus among patients with Helicobacter pylori infection [pmc:PMC4855822] |
| J Am Heart Assoc. | Transcriptional Profiling of Foam Cells Reveals Induction of Cavia (Guinea pig )anylate‐Binding Proteins Following Western Diet Acceleration of Atherosclerosis in the Absence of Global Changes in Inflammation [pmc:PMC4859273] |
| Journal of Applied Pharmaceutical Science | Organic Arsenical Exposure Stimulates Atherosclerosis through Oxidative Stress Increase and Adhesion Molecule Expression [admin:php] |
| Journal of Nutritional Biochemistry | Dietary rose hip exerts anti-atherosclerotic effects and increases nitric oxide-mediated dilation in ApoE-null mice [S0955286316301887] |
| Journal of Basic and Clinical Physiology and Pharmacology | Effects of altered thyroid states on oxidative stress parameters in rats [pubmed:27824612] |
| Journal of Functional Foods | Atherosclerosis-related inflammation and oxidative stress are improved by rice bran enzymatic extract [S1756464616302511] |
| Microvascular Research | Effects of insulin analogs as an add-on to metformin on cutaneous microcirculation in type 2 diabetic patients [pubmed:28954218] |
| Nutrients | Anti-Atherogenic Activity of Polyphenol-Rich Extract from Bee Pollen [pubmed:29258230] |
| Biochemical and Biophysical Research Communications | Oxidized low-density lipoprotein promotes osteoclast differentiation from CD68 positive mononuclear cells by regulating HMGB1 release [pubmed:29146189] |
| Journal of Surgical Research | THE FUNCTION OF SIALIC ACID AS A RADICAL SCAVENGER IN EXPERIMENTAL HYPOTHYROIDISM WITH AND WITHOUT HYPERLIPIDEMIA [10.14717/jsurgarts.2014.163] |
| biorxiv | Significant metabolic improvement by a water extract of olives: animal and human evidence [10.1101/151860] |
| Journal of nutritional biochemistry | Dietary rose hip exerts antiatherosclerotic effects and increases nitric oxide-mediated dilation in ApoE-null mice. [pubmed:28399420] |
| Nutrients | Yirui Capsules Alleviate Atherosclerosis by Improving the Lipid Profile and Reducing Inflammation in Apolipoprotein E-Deficient Mice [Pubmed:29382111] |
| Nutrition & Metabolism | Cold-pressed minke whale oil reduces circulating LDL/VLDL-cholesterol, lipid oxidation and atherogenesis in apolipoprotein E-deficient mice fed a Western … [Pubmed:29755576] |
| Journal of Functional Foods | Saponins of sea cucumber attenuate atherosclerosis in ApoE−/− mice via lipid-lowering and anti-inflammatory properties [10.1016:j.jff.2018.07.046] |
| Journal of Agricultural and Food Chemistry | Neuroprotective effect of hydroxytyrosol in experimental diabetic retinopathy: relationship with cardiovascular biomarkers [Pubmed:29278909] |
| Molecules | Protective Effect of Polyphenol-Rich Extract from Bee Pollen in a High-Fat Diet [Pubmed:29614743] |
| Lipids in Health and Disease | Lipingshu capsule improves atherosclerosis associated with lipid regulation and inflammation inhibition in apolipoprotein E–deficient mice [Pubmed:30064511] |
| Diabetes Metabolism Research and Reviews | Diabetic dyslipidaemia is associated with alterations in eNOS, caveolin‐1, and endothelial dysfunction in streptozotocin treated rats [Pubmed:29471582] |
| Diabetes & Metabolic Syndrome: Clinical Research & Reviews | Beyond LDL-c: The importance of serum oxidized LDL in predicting risk for type 2 diabetes in the middle-aged Asian Indians [Doi: 10.1016/j.dsx.2018.08.036] |
| 中国药理学通报 | 三七总皂苷对动脉粥样硬化小鼠的治疗作用 [] |
| Biomedicine & Pharmacotherapy | Effects of lycopene on metabolism of glycolipid in type 2 diabetic rats [Pubmed: 30551463] |
| Journal of Ethnopharmacology | Anti-diabetic properties of different fractions of Korean red ginseng [] |
| Chinese Pharmacological Bulletin中国药理学通报 | 地昔帕明对兔动脉斑块细胞凋亡及内质网应激的影响 [] |
| Document Version [] | |
| MEDICINE | Screening of schizophrenia associated miRNAs and the regulation of miR-320a-3p on integrin β1 [Pubmed: 30813134] |
| Pharmacological Research | Ethanol extracts of Danlou tablet attenuate atherosclerosis via inhibiting inflammation and promoting lipid effluent [Pubmed: 31181336] |
| Oxidative Medicine and Cellular Longevity | Combined Exercise Training Performed by Elderly Women Reduces Redox Indexes and Proinflammatory Cytokines Related to Atherogenesis [] |
| Nature Communications | ER-residential Nogo-B accelerates NAFLD-associated HCC mediated by metabolic reprogramming of oxLDL lipophagy [Pubmed: 31358770] |
| Clinical and Experimental Medicine | Senescence-associated miR-34a and miR-126 in middle-aged Indians with type 2 diabetes [Pubmed: 31732824] |
| Steroids | Serum 25 (OH) D concentration and its association with inflammation and oxidative stress in the middle-aged Indian healthy and diabetic subjects [Pubmed: 31672627] |
| ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | Zygophyllum album saponins prevent atherogenic effect induced by deltamethrin via attenuating arterial accumulation of native and oxidized LDL in rats [Pubmed: 32105945] |
| JOURNAL OF CARDIOVASCULAR PHARMACOLOGY | Ginsenoside Rb1 Alleviates Oxidative Low-Density Lipoprotein–Induced Vascular Endothelium Senescence via the SIRT1/Beclin-1/Autophagy Axis [Pubmed: 31658172] |
| Journal of Diabetes Research | Middle-Aged Indians with Type 2 Diabetes Are at Higher Risk of Biological Ageing with Special Reference to Serum CDKN2A [Pubmed: 32280716] |
| Acta Biochim Pol | Dietary Lactobacillus plantarum LS/07 and inulin in the management of chronic disease risk factors [Pubmed: 33108141] |
| Ecotoxicol Environ Saf | Permethrin induced arterial retention of native and oxidized LDL in rats by promoting inflammation, oxidative stress and affecting LDL receptors, and collagen … [Pubmed: 32911180] |
| Cell Death & Disease | Oxidized-LDL inhibits testosterone biosynthesis by affecting mitochondrial function and the p38 MAPK/COX-2 signaling pathway in Leydig cells [Pubmed: 32796811] |
| Nutrients | Ginseng-Induced Changes to Blood Vessel Dilation and the Metabolome of Rats [Pubmed: 32727012] |
| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | The Fungal Iron Chelator Desferricoprogen Inhibits Atherosclerotic Plaque Formation [Pubmed: 32635347] |
| Oxidative Medicine and Cellular Longevity | Improvement of Endothelial Dysfunction of Berberine in Atherosclerotic Mice and Mechanism Exploring through TMT-Based Proteomics [Pubmed: 32566106] |
| JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | The microvesicle/CD36 complex triggers a prothrombotic phenotype in patients with non‐valvular atrial fibrillation [Pubmed: 32510852] |
| Antioxidants | Nicotinamide Prevents Apolipoprotein B-Containing Lipoprotein Oxidation, Inflammation and Atherosclerosis in Apolipoprotein E-Deficient Mice [33233455] |
| MOLECULAR NUTRITION & FOOD RESEARCH | Digestive n©\6 Lipid Oxidation, a Key Trigger of Vascular Dysfunction and Atherosclerosis in the Western Diet: Protective Effects of Apple Polyphenols [33450108] |
| Scientific reports | Genetic deletion of Abcc6 disturbs cholesterol homeostasis in mice [33483533] |
| Scientific reports | Antibodies against apoB100 peptide 210 inhibit atherosclerosis in apoE-/-mice [33907226] |
| Journal of Food and Nutrition Research | Effect of Hibiscus sabdariffa Tea Extracts Intake on the Atherosclerosis Biomarkers in Adults [] |
| Journal of Food and Nutrition Research | Regulation of CD36 Gene Expression by Hibiscus Sabdariffa Tea Extracts to Affect the Atherosclerosis Biomarkers in Saudi Women [] |
| Eur J Nutr | The effects of long-term moderate exercise and Western-type diet on oxidative/nitrosative stress, serum lipids and cytokines in female Sprague Dawley rats [34319428] |
| Pediatr Gastroenterol Hepatol Nutr | The Relationship between Daily Fructose Consumption and Oxidized Low-Density Lipoprotein and Low-Density Lipoprotein Particle Size in Children with … [34557400] |
| J Mol Cell Cardiol | ILF3 is responsible for hyperlipidemia-induced arteriosclerotic calcification by mediating BMP2 and STAT1 transcription [34343541] |
| J Basic Clin Physiol Pharmacol | Anti-atherosclerotic activity of aqueous extract of Ipomoea batatas (L.) leaves in high-fat diet-induced atherosclerosis model rats [Pubmed:34986543] |
| Oxid Med Cell Longev | DNA Methyltransferase 3b Accelerates the Process of Atherosclerosis [Pubmed:35422896] |
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