血管紧张素转化酶2(ACE2)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Angiotensin I Converting Enzyme 2 (ACE2)
ACEH; ACEII; ACE-II; Peptidyl-Dipeptidase A; ACE-related carboxypeptidase; Angiotensin-converting enzyme homolog; Metalloprotease MPROT15
- 编号SEB886Ra
- 物种Rattus norvegicus (Rat，大鼠) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围0.78-50ng/mL
- 灵敏度最小可检测剂量小于等于0.31ng/mL.
- 样本类型Serum, plasma, tissue homogenates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 3032 ¥ 4332 ¥ 19494 ¥ 36822 ¥ 303240
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测血管紧张素转化酶2(ACE2)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的血管紧张素转化酶2(ACE2)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 99-105 | 102 |
| EDTA plasma(n=5) | 79-93 | 80 |
| heparin plasma(n=5) | 78-95 | 86 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的血管紧张素转化酶2(ACE2)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中血管紧张素转化酶2(ACE2)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 84-92% | 83-97% | 80-95% | 97-104% |
| EDTA plasma(n=5) | 80-102% | 91-101% | 78-104% | 83-105% |
| heparin plasma(n=5) | 88-104% | 85-92% | 98-105% | 78-101% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将血管紧张素转化酶2(ACE2)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的血管紧张素转化酶2(ACE2)与连接于固相载体上的抗体结合，然后加入生物素化的血管紧张素转化酶2(ACE2)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的血管紧张素转化酶2(ACE2)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| EPB886Ra61 | 血管紧张素转化酶2(ACE2)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB886Ra61 | 血管紧张素转化酶2(ACE2)活性蛋白 | Cell culture; Activity Assays. |
| APB886Ra01 | 血管紧张素转化酶2(ACE2)活性蛋白 | Cell culture; Activity Assays. |
| RPB886Ra01 | 血管紧张素转化酶2(ACE2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB886Ra01 | 血管紧张素转化酶2(ACE2)多克隆抗体 | WB; IHC; ICC; IP. |
| MAB886Ra23 | 血管紧张素转化酶2(ACE2)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB886Ra22 | 血管紧张素转化酶2(ACE2)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB886Ra24 | 血管紧张素转化酶2(ACE2)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB886Ra21 | 血管紧张素转化酶2(ACE2)单克隆抗体 | WB; IHC; ICC; IP. |
| SEB886Ra | 血管紧张素转化酶2(ACE2)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB886Ra | 血管紧张素转化酶2(ACE2)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Nature Medicine | Angiotensin-Converting Enzyme 2 Overexpression Remarkably Ameliorated Glomerular Injury in a Rat Model of Diabetic Nephropathy: A Comparison with ACE Inhibition [MolMed: 10_11_liu] |
| Journal of Cancer Therapy | Plasma Levels of Angiotensin-Converting Enzymes 1 and 2 and AGTR2 (T1247G and A5235G) Gene Polymorphisms Are Associated to Breast Cancer Progression. [Scirp: Source] |
| Reprod Toxicol.? | ACE2 activation by xanthenone prevents leptin-induced increases in blood pressure and proteinuria during pregnancy in Sprague-Dawley rats [Pubmed:25205467] |
| Intensive Care Medicine Experimental | Angiotensin converting enzymes in patients with acute respiratory distress syndrome [Content: 3] |
| Journal of Chromatography A | Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858] |
| Journal of Clinical Pharmacy and Therapeutics | Relationship between genetic variants of ACE2 gene and circulating levels of ACE2 and its metabolites. [pubmed:28895159] |
| Innate Immunity | Lipoxin A attenuates LPS-induced acute lung injury via activation of the ACE2-Ang-(1-7)-Mas axis [Pubmed:29969931] |
| Journal of Surgical Research | Impact of angiotensin-converting enzyme 2 levels on postoperative pneumonia after esophagectomy [Pubmed:29506841] |
| canadian journal of physiology and pharmacology | Could cardioprotective effect of ACE2 activator “diminazene aceturate” is more potent than ACE inhibitor “Enalapril” on acute myocardial infarction in rats? [Pubmed: 30840489] |
| Intensive Care Medicine | Angiotensin-converting enzymes in acute respiratory distress syndrome [] |
| BioMed Research International | AVE 0991 Attenuates Pyroptosis and Liver Damage after Heatstroke by Inhibiting the ROS-NLRP3 Inflammatory Signalling Pathway [] |
| Heart and vessels | Relationship between circulating levels of angiotensin-converting enzyme 2-angiotensin-(1–7)-MAS axis and coronary heart disease [Pubmed: 31359146] |
| Medicine | Alteration and association between serum ACE2/angiotensin (1-7)/Mas axis and oxidative stress in chronic kidney disease: A pilot study [Pubmed: 32756181] |
| American Journal of Respiratory and Critical Care Medicine | Equilibrium Angiotensin Metabolite Profiling in Patients with Acute Respiratory Distress Syndrome Indicates Angiotensin Converting Enzyme Inhibition [Pubmed: 32628511] |
| Clinical Research in Cardiology | Determinants of soluble angiotensin-converting enzyme 2 concentrations in adult patients with complex congenital heart disease [33280062] |
| Clinical & Translational Immunology | Pattern of circulating SARS©\CoV©\2©\specific antibody©\secreting and memory B©\cell generation in patients with acute COVID©\19 [33552508] |
| researchsquare | Novel transgenic mice with Cre-dependent co-expression of GFP and human ACE2: a safe tool for study of COVID-19 pathogenesis [33855640] |
| Viruses | Different Neutralization Sensitivity of SARS-CoV-2 Cell-to-Cell and Cell-Free Modes of Infection to Convalescent Sera [] |
| Sci Rep | Immunoreactivity of the SARS-CoV-2 entry proteins ACE-2 and TMPRSS-2 in murine models of hormonal manipulation, ageing, and cardiac injury [34907257] |
| European Journal of Clinical Investigation | Angiotensin‐converting enzyme 2 and Transmembrane protease serine 2 in female and male patients with end‐stage kidney disease [Pubmed:35366343] |
| Metabolites | Ramipril Reduces Acylcarnitines and Distinctly Increases Angiotensin-Converting Enzyme 2 Expression in Lungs of Rats [Pubmed:35448480] |
