载脂蛋白B100(APOB100)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Apolipoprotein B100 (APOB100)
Apo-B100
- 编号SEA603Ra
- 物种Rattus norvegicus (Rat，大鼠) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围62.5-4,000ng/mL
- 灵敏度最小可检测剂量小于等于28.2ng/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2554 ¥ 3648 ¥ 16416 ¥ 31008 ¥ 255360
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
-
产品包装(模拟)
-
产品包装(模拟)
-
实验结果图
-
标准曲线图
-
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测载脂蛋白B100(APOB100)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的载脂蛋白B100(APOB100)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 85-101 | 97 |
| EDTA plasma(n=5) | 90-98 | 93 |
| heparin plasma(n=5) | 88-101 | 92 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的载脂蛋白B100(APOB100)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中载脂蛋白B100(APOB100)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 87-94% | 80-93% | 86-95% | 84-94% |
| EDTA plasma(n=5) | 79-105% | 78-97% | 92-102% | 94-101% |
| heparin plasma(n=5) | 82-103% | 98-105% | 95-103% | 94-105% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将载脂蛋白B100(APOB100)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的载脂蛋白B100(APOB100)与连接于固相载体上的抗体结合，然后加入生物素化的载脂蛋白B100(APOB100)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的载脂蛋白B100(APOB100)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA603Ra01 | 载脂蛋白B100(APOB100)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA603Ra01 | 载脂蛋白B100(APOB100)多克隆抗体 | WB; IHC; ICC; IP. |
| MAA603Ra21 | 载脂蛋白B100(APOB100)单克隆抗体 | WB; IHC; ICC; IP. |
| WEA603Ra | 载脂蛋白B100(APOB100)检测试剂盒(酶联免疫吸附试验法,宽范围) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA603Ra | 载脂蛋白B100(APOB100)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA603Ra | 载脂蛋白B100(APOB100)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| PLoS One | Enhancement of Naringenin Bioavailability by Complexation with Hydroxypropoyl-β-Cyclodextrin [Plosone: 0018033] |
| Autonomic Neuroscience | Chemical sympathectomy induces arterial accumulation of native and oxidized LDL in hypercholesterolemic rats [ScienceDirect: S1566070211004152] |
| Toxicology | iTRAQ-based proteomic profiling of human serum reveals down-regulation of platelet basic protein and apolipoprotein B100 in patients with hematotoxicity induced by chronic occupational benzene exposure [ScienceDirect: S0300483X11004628] |
| Atherosclerosis | Up-reCavia (Guinea pig )lation of Hnf1α gene expression in the liver of rats with experimentally induced chronic renal failure – A possible link between circulating PCSK9 and triacylglycerol concentrations [Pubmed:26978583] |
| Proteomics Clinical Applications | Comparative mass spectrometric and immunoassay‐based proteome analysis in serum of Duchenne muscular dystrophy patients [Pubmed:26680509] |
| molecular nutrition & food research | Alleviating VLDL overproduction is an important mechanism for Laminaria japonica polysaccharide to inhibit atherosclerosis in LDLr-/- mice with diet-induced insulin resistance. [pubmed:27928899] |
| PROTEOMICS - Clinical Applications | Comparative mass spectrometric and immunoassay-based proteome analysis in serum ofDuchenne muscular dystrophy patients. [pubmed:26680509] |
| Biosci Biotechnol Biochem. | Choline and betaine ameliorate liver lipid accumulation induced by vitamin B6 deficiency in rats. [pubmed:27696964] |
| Journal of Nutritional Science and Vitaminology | Accumulation of Liver Lipids Induced by Vitamin B6 Deficiency Was Effectively Ameliorated by Choline and, to a Lesser Extent, Betaine [Pubmed: 30814419] |
| ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | Zygophyllum album saponins prevent atherogenic effect induced by deltamethrin via attenuating arterial accumulation of native and oxidized LDL in rats [Pubmed: 32105945] |
| Ecotoxicol Environ Saf | Permethrin induced arterial retention of native and oxidized LDL in rats by promoting inflammation, oxidative stress and affecting LDL receptors, and collagen … [Pubmed: 32911180] |
| Nat Commun | GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity [34853313] |
| Clinical Nutrition | Postprandial dyslipidemia after a standardized high-fat meal in BMI-matched healthy individuals, and in subjects with prediabetes or Type 2 diabetes [34656950] |
| Nano Research | Immunomodulatory hybrid bio-nanovesicle for self-promoted photodynamic therapy [] |
