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基质金属蛋白酶13(MMP13)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Matrix Metalloproteinase 13 (MMP13)

CLG3; Collagenase 3

  • 基质金属蛋白酶13(MMP13)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 基质金属蛋白酶13(MMP13)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 基质金属蛋白酶13(MMP13)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA099Ra.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测基质金属蛋白酶13(MMP13),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的基质金属蛋白酶13(MMP13)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 99-105 102
heparin plasma(n=5) 80-98 86

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的基质金属蛋白酶13(MMP13),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中基质金属蛋白酶13(MMP13)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 96-104% 93-101% 86-94% 85-96%
heparin plasma(n=5) 78-92% 80-93% 94-103% 91-102%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将基质金属蛋白酶13(MMP13)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的基质金属蛋白酶13(MMP13)与连接于固相载体上的抗体结合,然后加入生物素化的基质金属蛋白酶13(MMP13)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的基质金属蛋白酶13(MMP13)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
EPA099Ra61 基质金属蛋白酶13(MMP13)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA099Ra01 基质金属蛋白酶13(MMP13)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA099Ra02 基质金属蛋白酶13(MMP13)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA099Ra61 基质金属蛋白酶13(MMP13)活性蛋白 Cell culture; Activity Assays.
PAA099Ra01 基质金属蛋白酶13(MMP13)多克隆抗体 WB; IHC; ICC; IP.
PAA099Ra02 基质金属蛋白酶13(MMP13)多克隆抗体 WB; IHC; ICC; IP.
LAA099Ra71 基质金属蛋白酶13(MMP13)多克隆抗体(生物素标记) WB; IHC; ICC.
SEA099Ra 基质金属蛋白酶13(MMP13)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA099Ra 基质金属蛋白酶13(MMP13)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA099Ra 基质金属蛋白酶13(MMP13)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease Hyaluronan reduces inflammation in experimental arthritis by modulating TLR-2 and TLR-4 cartilage expression [ScienceDirect: S0925443911001323]
Journal of Physiology and Biochemistry Protective effects of curcumin, α-lipoic acid, and N-acetylcysteine against carbon tetrachloride-induced liver fibrosis in rats [SpringerLink: h875w73171w2147w]
FEBS Journal Adenosine A2a Receptor Activation and Hyaluronan Fragment Inhibition Reduce Inflammation in Mouse Articular Chondrocytes Stimulated with Il-1beta [Wiley: source]
Arthritis & Rheumatism Interruption of glycosphingolipid synthesis enhances osteoarthritis development in mice [Wiley: source]
Gene Hyaluronan in part mediates IL-1beta-induced inflammation in mouse chondrocytes by up-regulating CD44 receptors [ScienceDirect: S0378111911007347]
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source]
Arthritis Rheumatol Identification of Alpha 2 Macroglobulin (A2M) as a master inhibitor of cartilage degrading factors that attenuates post-traumatic osteoarthritis progression. [Pubmed: 24578232]
Arthritis & Rheumatology Identification of Alpha 2 Macroglobulin (A2M) as a master inhibitor of cartilage degrading factors that attenuates post-traumatic osteoarthritis progression [Onlinelibrary: art.38576]
Thromb Haemost. Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke [Pubmed:24671655]
Arthritis & Rheumatol Identification of α2-Macroglobulin as a Master Inhibitor of Cartilage-Degrading Factors That Attenuates the Progression of Posttraumatic Osteoarthritis [Pubmed:24578232]
Inflammation.? Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis [Pubmed:25448261]
Cellular Physiology and Biochemistry Inverse Regulation of Early and Late Chondrogenic Differentiation by Oxygen Tension Provides Cues for Stem Cell-Based Cartilage Tissue Engineering [Pubmed:25632940]
Osteoarthritis and Cartilage Depletion of gangliosides enhances cartilage degradation in mice [Sciencedirect:S1063458413010364]
Biol Res The role of MCP-1-CCR2 ligand-receptor axis in chondrocyte degradation and disease progress in knee osteoarthritis [PubMed: 26578310]
PLoS One Interleukin-1 Receptor Antagonist Has a Novel Function in the Regulation of Matrix Metalloproteinase-13 Expression [PubMed: 26474296]
Cell Mol Immunol  Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica [Pubmed:27157496]
Scientific Reports Chondrogenic Potential of Peripheral Blood Derived Mesenchymal Stem Cells Seeded on Demineralized Cancellous Bone Scaffolds [pubmed:27821864]
International Journal of Contemporary Medical Research Matrix Metalloproteinase-13 (MMP-13): A Novel Tumor Marker for Diagnosis of Breast Carcinoma [uploads:7]
Scientific Reports Olive and grape seed extract prevents post-traumatic osteoarthritis damages and exhibits in vitro anti IL-1β activities before and after oral consumption. [pubmed:27640363]
Photodermatol Photoimmunol Photomed. Protective effects of Aloe sterols against UVB‐induced photoaging in hairless mice [pubmed:27995657]
Molecular Medicine Reports Icariin inhibits MMP‑1, MMP‑3 and MMP‑13 expression through MAPK pathways in IL‑1β‑stimulated SW1353 chondrosarcoma cells [pubmed:28447732]
bmc musculoskeletal disorders Strontium ranelate causes osteophytes overgrowth in a model of early phase osteoarthritis. [pubmed:28187731]
Journal of Cellular Physiology Increased Chondrogenic Potential of Mesenchymal Cells From Adipose Tissue Versus Bone Marrow‐Derived Cells in Osteoarthritic In Vitro Models [pubmed:27739057]
BMC Musculoskeletal Disorders Establishment of rat ankle post-traumatic osteoarthritis model induced by malleolus fracture [pubmed:29149841]
Journal of Applied Animal Research Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts:an in vitro approach to bovine digital dermatitis [10.108:09712119.2017.1335208]
Alexandria Journal of Medicine L-Carnitine ameliorates knee lesions in mono-iodoacetate induced osteoarthritis in rats [10.1016:j.ajme.2016.03.002]
National Journal of Integrated Research in Medicine Matrix Metalloproteinase-13 Can Be Used As an Independent Diagnostic and Prognostic Marker for Breast Carcinoma. [AN=123116960]
Molecular Medicine Reports Effects of GGCX overexpression on anterior cruciate ligament transection-induced osteoarthritis in rabbits [Pubmed:29257344]
Journal of Applied Animal Research  Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts: an approach to bovine digital dermatitis [10.1080:09712119.2017.1335208]
Osteoarthritis Cartilage Coordinated existence of multiple gangliosides is required for cartilage metabolism [Pubmed: 30471358]
PLoS One Co-culture of osteochondral explants and synovial membrane as in vitro model for osteoarthritis [Pubmed: 30939166]
Pathobiology Suppressed Expression but Not Activity of Collagenases MMP-1 and MMP-13 in Human Renal Carcinoma [Pubmed: 31129674]
Bone DAla2GIP antagonizes H2O2-induced chondrocyte apoptosis and inflammatory factor secretion [Pubmed: 31283994]
Food & Function A dietary polysaccharide from Eucheuma cottonii downregulates proinflammatory cytokines and ameliorates osteoarthritis-associated cartilage degradation in obese … []
JOURNAL OF CELLULAR BIOCHEMISTRY Teneligliptin inhibits IL‐1β‐induced degradation of extracellular matrix in human chondrocytes [Pubmed: 32162384]
Bone Research Effects of tetrahedral framework nucleic acid/wogonin complexes on osteoarthritis [Pubmed: 32047705]
Scientific Reports Matrix metallopeptidase expression and modulation by transforming growth factor-β1 in equine endometrosis [Pubmed: 31980722]
Nutrients Chondroprotective Properties of Human-Enriched Serum Following Polyphenol Extract Absorption: Results from an Exploratory Clinical Trial [Pubmed: 31888255]
Nature Communications Vulnerability of progeroid smooth muscle cells to biomechanical forces is mediated by MMP13 [Pubmed: 32807790]
Molecular Medicine Reports MicroRNA-1 promotes cartilage matrix synthesis and regulates chondrocyte differentiation via post-transcriptional suppression of Ihh expression [Pubmed: 32705199]
RESEARCH SQUARE Experimental Observation of the Sequence of Tibial Plateau Chondrocyte and Matrix Degeneration in Spontaneous Osteoarthritis in Guinea Pigs []
Hyaluronic Acid versus Alpha-2-Macroglobulin Intra-Articular Injections for Amelioration of Knee Posttraumatic Osteoarthritis: A Rat Model []
Loss of autophagy in tibial plateau chondrocytes causes increased apoptosis of chondrocytes in spontaneous osteoarthritis of guinea pigs []
Food Funct Undenatured type II collagen prevents and treats the osteoarthritis and motor function degradation in T2DM patients and db/db mice [33890588]
Nutrients Metabolic and Anti-Inflammatory Protective Properties of Human Enriched Serum Following Artichoke Leaf Extract Absorption: Results from an Innovative Ex Vivo … [34444810]
Sci Rep Liraglutide, a glucagon-like peptide 1 receptor agonist, exerts analgesic, anti-inflammatory and anti-degradative actions in osteoarthritis [Pubmed:35091584]
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