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Toll样受体4(TLR4)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Toll Like Receptor 4 (TLR4)

CD284; TOLL; HToll

  • Toll样受体4(TLR4)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • Toll样受体4(TLR4)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • Toll样受体4(TLR4)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA753Ra.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测Toll样受体4(TLR4),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将Toll样受体4(TLR4)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的Toll样受体4(TLR4)与连接于固相载体上的抗体结合,然后加入生物素化的Toll样受体4(TLR4)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的Toll样受体4(TLR4)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
RPA753Ra01 Toll样受体4(TLR4)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA753Ra01 Toll样受体4(TLR4)多克隆抗体 WB; IHC
LAA753Ra71 Toll样受体4(TLR4)多克隆抗体(生物素标记) WB; IHC; ICC.
LAA753Ra81 Toll样受体4(TLR4)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
MAA753Ra21 Toll样受体4(TLR4)单克隆抗体 WB; IHC; ICC; IP.
SEA753Ra Toll样受体4(TLR4)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA753Ra Toll样受体4(TLR4)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA753Ra01 Toll样受体4(TLR4)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Chinese Medical Journal Expression of soluble Toll-like receptors in pleural effusions [CMJ: 2010823483999506297]
Journal of Maternal-Fetal and Neonatal Medicine Amniotic fluid soluble Toll-like receptor 4 in pregnancies complicated by preterm prelabor rupture of the membranes [Informa: 14767058.2011.626821]
PLoS One Baicalein Reduces Airway Injury in Allergen and IL-13 Induced Airway Inflammation [PubMed: PMC3639905]
Clin Res Hepatol Gastroenterol. The association between precancerous gastric lesions and serum pepsinogens, serum gastrin, vascular endothelial growth factor, serum interleukin-1 Beta, serum toll-like receptor-4 levels and Helicobacter pylori Cag A status [Pubmed: 23137754]
Journal of Clinical Microbiology Toll-Like Receptor 2 in Serum: a Potential Diagnostic Marker of Prosthetic Joint Infection? [Pubmed: 24478497]
Surgical Endoscopy CO2 pneumoperitoneum increases secretory IgA levels in the gut compared with laparotomy in an experimental animal model. [Pubmed: 24442682]
Experimental and therapeutic medicine Soluble toll-like receptor 4 reversed attenuating effect of Chinese herbal Xiao-Qing-Long-Tang on allergen induced nerve growth factor and thymic stromal lymphopoietin [Pubmed: 24223644]
Free Radical Biology and Medicine Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitis [Pubmed:24992835]
Respiratory Virology and Immunogenicity Advances in Experimental Medicine and Biology Cytokines and Toll-Like Receptors in the Immune Response to Influenza Vaccination [Pubmed:25248345]
Molecular and Cellular Biochemistry Beta-arrestin-2 negatively modulates inflammation response in mouse chondrocytes induced by 4-mer hyaluronan oligosaccharide [Pubmed:25318610]
Journal of Ginseng Research Effects of Korean Red Ginseng (Panax ginseng), urushiol (Rhus vernicifera Stokes), and probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus acidophilus R0052) on the gut–liver axis of alcoholic liver disease [Pubmed:25378990]
BMC Immunology The discriminative capacity of soluble Toll-like receptor (sTLR)2 and sTLR4 in inflammatory diseases [Pubmed:25406630]
Gastroenterology Research and Practice Procoagulatory State in Inflammatory Bowel Diseases Is Promoted by Impaired Intestinal Barrier Function [Pubmed:25767508]
Eur J Gastroenterol Hepatol Efficacy of rifaximin on circulating endotoxins and cytokines in patients with nonalcoholic fatty liver disease [PubMed: 26043290]
Pathol Biol (Paris) Cathelicidin related antimicrobial peptide, laminin, Toll-like receptors and chemokines levels in experimental hypersensitivity pneumonitis in mice [PubMed: 25834936]
Journal of Food Science  Effects of Hyriopsis cumingii Polysaccharides on Mice Immunologic Receptor, Transcription Factor, and Cytokine [Pubmed:27061742]
Plos One Soluble Toll-Like Receptors 2 and 4 in Cerebrospinal Fluid of Patients with Acute Hydrocephalus followingAneurysmal Subarachnoid Haemorrhage. [Pubmed:27223696]
Open Forum Infect Dis The Effect of Rosuvastatin on Markers of Immune Activation in Treatment-Naive Human Immunodeficiency Virus-Patients [Pubmed:26835476]
Animal Feed Science and Technology Impact of Saccharomyces cerevisiae fermentation product and subacute ruminal acidosis on production, inflammation, and fermentation in the rumen and hindCavia (Guinea pig )t of dairy cows [science:S0377840115300493]
european journal of pharmacology Garlic active constituent s-allyl cysteine protects against lipopolysaccharide-induced cognitive deficits in the rat: Possible involved mechanisms. [pubmed:27915041]
european journal of pharmacology S-allyl cysteine ameliorates cognitive deficits in streptozotocin-diabetic rats via suppression of oxidative stress, inflammation, and acetylcholinesterase. [pubmed:27887948]
Psychopharmacology (Berl) Ellagic acid ameliorates learning and memory deficits in a rat model of Alzheimer's disease: an exploration of underlying mechanisms. [pubmed:28303372]
ЭНИ Забайкальский медицинский вестник НОСИТЕЛЬСТВО ПОЛИМОРФИЗМА Thr399Ile и Asp299Gly ГЕНА TLR4 И УРОВЕНЬ TLR4 В ОПУХОЛЕВОЙ ТКАНИ У БОЛЬНЫХ ПРОЛИФЕРАТИВНЫМИ ЗАБОЛЕВАНИЯМИ МОЛОЧНОЙ ЖЕЛЕЗЫ [8.pdf]
biomedicine & pharmacotherapy Acetyl-l-carnitine protects dopaminergic nigrostriatal pathway in 6-hydroxydopamine-induced model of Parkinson's disease in the rat. [pubmed:28199883]
Journal For Neurovirology Soluble TLR2 and 4 concentrations in cerebrospinal fluid in HIV/SIV-relatedneuropathological conditions. [pubmed:27882497]
Acta Orthopaedica Toll-like receptors and their soluble forms differ in the knee and thumb basal osteoarthritic joints [pubmed:28093922]
Environmental Toxicology and Pharmacology Ameliorative potential of linagliptin and/or calcipotriol on bleomycin-induced lung fibrosis: In vivo and in vitro study [pubmed:28192751]
Inflammation Toll-Like Receptor 4 Inhibition Improves Oxidative Stress and Mitochondrial Health in Isoproterenol-Induced Cardiac Hypertrophy in Rats [pubmed:28690610]
Comparative Immunology, Microbiology and Infectious Diseases Probiotic Lactobacillus acidophilus bacteria or synthetic TLR2 agonist boost the growth of chicken embryo intestinal organoids in cultures comprising epithelial cells and myofibroblasts [DOI: 10.1016/j.cimid.2017.06.002]
Comparative Immunology, Microbiology and Infectious Diseases Probiotic Lactobacillus acidophilus bacteria or synthetic TLR2 agonist boost the growth of chicken embryo intestinal organoids in cultures comprising epithelial cells and myofibroblasts [10.1016/j.cimid.2017.06.002]
Microbial Pathogenesis Protective effects of sinomenine against LPS-induced inflammation in piglets. [pubmed:28757275]
Cytokine Soy isoflavone genistein attenuates lipopolysaccharide-induced cognitive impairments in the rat via exerting anti-oxidative and anti-inflammatory effects [pubmed:29102164]
Biomedicine & pharmacotherapy Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanisms [pubmed:28061403]
Frontiers in Immunology The soluble Form of Toll-like receptor 2 is elevated in serum of Multiple sclerosis Patients: a novel Potential Disease Biomarker [Pubmed:29593720]
Experimental Gerontology Specific alterations in the circulating levels of the SIRT1, TLR4, and IL7 proteins in patients with dementia [Pubmed:30071285]
Neurotoxicity Research Protective Effects of Fibroblast Growth Factor 21 Against Amyloid-Beta-Induced Toxicity in SH-SY5Y Cells [Pubmed:29869772]
European Journal of Pharmacology Rutin, a quercetin glycoside, alleviates acute endotoxemic kidney injury in C57BL/6 mice via suppression of inflammation and up-regulation of antioxidants and SIRT1 [Pubmed:29920283]
Environmental Toxicology and Pharmacology Crocin reverses unilateral renal ischemia reperfusion injury-induced augmentation of oxidative stress and toll like receptor-4 activity [Pubmed:29625388]
Physiology & Behavior  Linagliptin potentiates the effect of l-dopa on the behavioural, biochemical and immunohistochemical changes in experimentally-induced Parkinsonism: Role of toll … [Pubmed:29410018]
International Journal of Gynecology & Obstetrics Increased serum levels of high mobility group protein B1 and calprotectin in pre‐eclampsia [Pubmed:29569400]
Journal of Maternal-Fetal & Neonatal Medicine Increased TLR4 and TREM-1 expression on monocytes and neutrophils in preterm birth: further evidence of a proinflammatory state [Pubmed:29534643]
ТLR4-РІВНІ У ХВОРИХ НА ПОДАГРУ: ЗВ'ЯЗОК З АКТИВНІСТЮ ЗАХВОРЮВАННЯ [:]
Journal of Medical Virology Combined effect of anti‐high‐mobility group box‐1 monoclonal antibody and peramivir against influenza A virus‐induced pneumonia in mice [Pubmed: 30281823]
Metabolic Brain Disease Berberine ameliorates lipopolysaccharide-induced learning and memory deficit in the rat: insights into underlying molecular mechanisms [Pubmed: 30456649]
Experimental and Therapeutic Medicine The HMGB1‑RAGE/TLR‑TNF‑α signaling pathway may contribute to kidney injury induced by hypoxia []
Immunopharmacology and immunotoxicology Protective effect of sesamin in lipopolysaccharide-induced mouse model of acute kidney injury via attenuation of oxidative stress, inflammation, and apoptosis [Pubmed: 30488751]
International Immunopharmacology S-allyl cysteine protects against lipopolysaccharide-induced acute kidney injury in the C57BL/6 mouse strain: Involvement of oxidative stress and inflammation [Pubmed: 30665040]
Biomedicine & Pharmacotherapy Opuntioside, opuntiol and its metallic nanoparticles attenuate adjuvant-induced arthritis: Novel suppressors of Toll-like receptors-2 and-4 []
International Journal of Obesity Circulating microbiota-derived metabolites: a “liquid biopsy? [Pubmed: 31388096]
Scientific Reports Deregulation of TLR4 signaling pathway characterizes Bicuspid Aortic valve syndrome [Pubmed: 31363123]
Life Sciences Crocin attenuates lung inflammation and pulmonary vascular dysfunction in a rat model of bleomycin-induced pulmonary fibrosis [Pubmed: 31465731]
Respiratory physiology & neurobiology Targeting oxidative stress, proinflammatory cytokines, apoptosis and toll like receptor 4 by empagliflozin to ameliorate bleomycin-induced lung fibrosis [Pubmed: 31600583]
Thrombosis and haemostasis Particulate Shiga Toxin 2 in Blood is Associated to the Development of Hemolytic Uremic Syndrome in Children [Pubmed: 31858520]
J Ethnopharmacol Effects of fermented ginseng on the gut microbiota and immunity of rats with antibiotic-associated diarrhea [Pubmed: 33217518]
International Journal of Molecular Sciences Relationship between IL-8 Circulating Levels and TLR2 Hepatic Expression in Women with Morbid Obesity and Nonalcoholic Steatohepatitis [Pubmed: 32545403]
NEUROPSYCHOBIOLOGY Diosgenin Attenuates Cognitive Impairment in Streptozotocin-Induced Diabetic Rats: Underlying Mechanisms [Pubmed: 32526752]
journal of biochemical and molecular toxicology S‐allyl cysteine, an active ingredient of garlic, attenuates acute liver dysfunction induced by lipopolysaccharide/ d‐galactosamine in mouse: Underlying mechanisms [Pubmed: 32453893]
microbial pathogenesis Protective effect of diosgenin on LPS/D-Gal-induced acute liver failure in C57BL/6 mice [Pubmed: 32389705]
Sciences of Europe FEATURES OF MYOCARDIAL REMODELING IN MEN WITH POST-INFARCTION CARDIOSCLEROSIS AND ITS RELATIONSHIP WITH TOLL-LIKE … []
Putative anti-inflammatory, antioxidant, and anti-apoptotic roles of the natural tissue guardian methyl palmitate against isoproterenol-induced myocardial … []
The role of Toll-like receptors-4 in the pathogenesis of development of anemia of inflammation in young children []
Clin Oral Investig Evaluation of heat shock protein 70 and toll-like receptor 4 expression in gingival crevicular fluid in response to orthodontic forces [34091792]
Eksp Klin Gastroenterol Tissue transglutaminase is involved in the inflammatory processes of active chronic gastritis [17539352]
Iranian Journal of Medical Sciences Esculetin Alleviates Acute Liver Failure following Lipopolysaccharide/D-Galactosamine in Male C57BL/6 Mice []
Acta Obstet Gynecol Scand A value of soluble Toll‐like receptor 2 and 4 in vaginally obtained amniotic fluid for the prediction of histological chorioamnionitis [34244993]
Nutrients Expression of Jejunal Taste Receptors in Women with Morbid Obesity [34371946]
Biomed Pharmacother Nifuroxazide-loaded cubosomes exhibit an advancement in pulmonary delivery and attenuate bleomycin-induced lung fibrosis by regulating the STAT3 and … [Pubmed:35220029]
Biomed Pharmacother Coomassie brilliant blue G-250 dye attenuates bleomycin-induced lung fibrosis by regulating the NF-κB and NLRP3 crosstalk: A novel approach for filling an … [Pubmed:35202914]
Frontiers in Pharmacology Carbocisteine as a Modulator of Nrf2/HO-1 and NFκB Interplay in Rats: New Inspiration for the Revival of an Old Drug for Treating Ulcerative Colitis. Front … [Pubmed:35754464]
Processes Lipocalin, Resistin and Gut Microbiota-Derived Propionate Could Be Used to Predict Metabolic Bariatric Surgery Selected Outcomes []
Biomolecules Vascular Calcification and the Gut and Blood Microbiome in Chronic Kidney Disease Patients on Peritoneal Dialysis: A Pilot Study []
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