干扰素α(IFNa)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Interferon Alpha (IFNa)
IFNA1; IFL; LeIF D; IFN; IFN-Alpha; IFNA13; IFN-A; IFNAP22; IFN-Alpha 1b; Interferon Alpha 1b; Interferon, Leukocytic; IFN, Leukocyte; Interferon alpha-D
- 编号SEA033Si
- 物种Rhesus monkey (Simian，恒河猴) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围15.6-1,000pg/mL
- 灵敏度最小可检测剂量小于等于6.4pg/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 3402 ¥ 4860 ¥ 21870 ¥ 41310 ¥ 340200
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
-
产品包装(模拟)
-
产品包装(模拟)
-
实验结果图
-
标准曲线图
-
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测干扰素α(IFNa)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的干扰素α(IFNa)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 86-93 | 89 |
| EDTA plasma(n=5) | 79-91 | 82 |
| heparin plasma(n=5) | 86-94 | 89 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的干扰素α(IFNa)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中干扰素α(IFNa)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 87-96% | 90-101% | 86-96% | 78-96% |
| EDTA plasma(n=5) | 83-95% | 98-105% | 84-92% | 92-105% |
| heparin plasma(n=5) | 89-96% | 85-94% | 84-94% | 85-93% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将干扰素α(IFNa)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的干扰素α(IFNa)与连接于固相载体上的抗体结合，然后加入生物素化的干扰素α(IFNa)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的干扰素α(IFNa)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Rhesus monkey (Simian，恒河猴) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA033Si01 | 干扰素α(IFNa)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA033Si01 | 干扰素α(IFNa)多克隆抗体 | WB; IHC; ICC; IP. |
| MAA033Si21 | 干扰素α(IFNa)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA033Si | 干扰素α(IFNa)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| IEA033Si | 干扰素α(IFNa)检测试剂盒(酶联免疫吸附试验法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA033Si | 干扰素α(IFNa)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA033Si | 干扰素α(IFNa)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Journal of Animal and Veterinary Advances | Tryptophan Stimulates Immune Response in Broiler Chickens Challenged with Infectious Bursal Disease Vaccine [Medwell: 2010610616] |
| Journal of Animal and Veterinary Advances | Immunostimulatory Effects of Arginine in Broiler Chickens Challenged with Vaccine Strain of Infectious Bursal Disease Virus [Medwell: 2010594600] |
| Ann Rheum Dis. | The cathelicidins LL-37 and rCRAMP are associated with pathogenic events of arthritis in humans and rats [PubMed: 23172753] |
| Nanomedicine | Efficient intracellular delivery of siRNA with a safe multitargeted lipid-based nanoplatform [PubMed: 23394132] |
| International journal of experimental pathology | Experimental rat model for alcohol-induced osteonecrosis of the femoral head [PubMed: PMC3781777] |
| Antiviral Research | A recombinant adenovirus bicistronically expressing porcine interferon-α and interferon-γ enhances antiviral effects against foot-and-mouth disease virus [ScienceDirect: S0166354214000266] |
| British Journal of Pharmacology | Andrographolide exerts anti-hepatitis C virus activity by up-regulating haeme oxygenase-1 via the p38 MAPK/Nrf2 pathway in human hepatoma cells [Onlinelibrary: bph.12440] |
| BMC Cancer | High efficiency of alphaviral gene transfer in combination with 5-fluorouracil in a mouse mammary tumor model [Pubmed:24950740] |
| PLoS One. | Respiratory Mechanics and Plasma Levels of Tumor Necrosis Factor Alpha and Interleukin 6 Are Affected by Gas Humidification during Mechanical Ventilation in Dogs [Pubmed:25036811] |
| Veterinary Research | Infection of equine monocyte-derived macrophages with an attenuated equine infectious anemia virus (EIAV) strain induces a strong resistance to the infection by a virulent EIAV strain [Pubmed:25106750] |
| Asian Pac J Trop Med | Effect of SecinH3 on lung injury induced by sepsis of rats [PubMed: 26706678] |
| PLoS One | siRNA Targeting the 2A pro Genomic Region Prevents Enterovirus 71 Replication In Vitro [Pubmed:26886455] |
| Plos One | Sulforaphane Suppresses Hepatitis C Virus Replication by Up-ReCavia (Guinea pig )lating Heme Oxygenase-1 Expressionthrough PI3K/Nrf2 Pathway. [Pubmed:27023634] |
| PLoS One. | Vaccination with recombinant Mycobacterium tuberculosis PknD attenuates bacterial dissemination to the brain in Cavia (Guinea pig )inea pigs. [Pubmed:23776655] |
| Veterinary microbiology | Impact of porcine reproductive and respiratory syndrome virus and porcine circovirus-2 infection on the potency of the classical swine fever vaccine (LOM strain). [pubmed:27599928] |
| International Journal of Clinical and Experimental Pathology | Host miR-146a promotes replication of human cytomegalovirus by suppressing type I IFN response in MRC-5 cells [files:ijcep0036479.pdf] |
| Scandinavian Journal of Immunology | Serum Proteomics Analysis in Rats of Immunosuppression Induced by Chronic Stress. [pubmed:27384769] |
| Saudi Journal of Gastroenterology | Deoxyschizandrin suppresses dss-induced ulcerative colitis in mice. [pubmed:27976641] |
| Biochemical and Biophysical Research Communications | MicroRNA-548j inhibits type I interferon production by targeting ZBTB11 in patients with chronic hepatitis B [pubmed:28342861] |
| Virology | The down-regulation of casein kinase 1 alpha as a host defense response against infectious bursal disease virus infection [pubmed:28988058] |
| Antiviral Research | Coinjection of a vaccine and anti-viral agents can provide fast-acting protection from foot-and-mouth disease [pubmed:28454913] |
| Molecular Therapy. Methods & Clinical Development | Anti-high mobility group box 1 antibody ameliorates albuminuria in MRL/lprlupus-prone mice [pubmed:28649578] |
| Journal of Virology | Fucoidan from Fucus vesiculosus suppresses hepatitis B virus replication by enhancing extracellular signal-regulated Kinase activation. [pubmed:28915824] |
| Clinical Immunology | Toll-like receptor 9 ligands increase type I interferon induced B-cell activating factor expression in chronic rhinosinusitis with nasal polyposis [Pubmed:30056130] |
| International forum of allergy & rhinology | Role of Toll‐like receptor 9 signaling on activation of nasal polyp–derived fibroblasts and its association with nasal polypogenesis [Pubmed:29901280] |
| LUPUS | Collagen triple helix repeat containing-1: a novel biomarker associated with disease activity in Systemic lupus erythematosus [Pubmed: 30336754] |
| Journal of Pineal Research | Role of melatonin in sleep deprivation‐induced intestinal barrier dysfunction in mice [Pubmed: 30929267] |
| Veterinary Immunology and Immunopathology | Differential expression of type I interferon mRNA and protein levels induced by virulent Marek's disease virus infection in chickens [] |
| international immunopharmacology | Adenovirus-mediated down-regulation of miR-21-5p alleviates experimental autoimmune uveoretinitis in mice [Pubmed: 31228813] |
| FASEB Journal | Scavenger receptor A impairs interferon response to HBV infection by limiting TRAF 3 ubiquitination through recruiting OTUB 1 [] |
| Transboundary and Emerging Diseases | Pathogenicity of porcine reproductive and respiratory syndrome virus (ORF5 RFLP 1–7–4 viruses) in China [Pubmed: 32187856] |
| archives of virology | A flagellin-adjuvanted inactivated porcine epidemic diarrhea virus (PEDV) vaccine provides enhanced immune protection against PEDV challenge in piglets [Pubmed: 32253616] |
| Autophagy | LDHB inhibition induces mitophagy and facilitates the progression of CSFV infection [Pubmed: 32924761] |
| THERIOGENOLOGY | Porcine soluble CD83 alleviates LPS-induced abortion in mice by promoting Th2 cytokine production, Treg cell generation and trophoblast invasion [Pubmed: 32810792] |
| Int Immunopharmacol | BNIP3 deletion ameliorated enterovirus 71 infection-induced hand, foot and mouth disease via inhibiting apoptosis, autophagy, and inflammation in mice [Pubmed: 32717566] |
| Molecular Medicine Reports | Aquaporin‑4 deletion ameliorates enterovirus 71 infection in mice [Pubmed: 32582978] |
| Virulence | Determination of antiviral action of Long Non-coding RNA loc107051710 During Infectious Bursal Disease Virus Infection due to Enhancement of Interferon … [] |
| РОССИЙСКАЯ СЕЛЬСКОХОЗЯЙСТВЕННАЯ НАУКА | ГОРМОНАЛЬНЫЙ И ЦИТОКИНОВЫЙ ПРОФИЛЬ КРОВИ МОЛОЧНЫХ КОРОВ В РАННИЙ ПЕРИОД ГЕСТАЦИИ [] |
| Communications Biology | Influenza virus neuraminidase regulates host CD8+ T-cell response in mice [33293641] |
| Animals | Concentration-Dependent Type 1 Interferon-Induced Regulation of MX1 and FABP3 in Bovine Endometrial Explants [33494350] |
| Int J Dermatol | IFN©\¦Á and TNF©\¦Á serum levels and their association with disease severity in Egyptian children and adults with alopecia areata [34008204] |
| Dig Dis Sci | Orexin A Suppresses the Expression of Exosomal PD-L1 in Colon Cancer and Promotes T Cell Activity by Inhibiting JAK2/STAT3 Signaling Pathway [34097168] |
| Clinical findings of Talaromyces marneffei infection among patients with anti-interferon-¦Ã immunodeficiency: a prospective cohort study [34144700] | |
| ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | Chronic developmental exposure to low-dose ([C8mim][PF6]) induces neurotoxicity and behavioural abnormalities in rats [34555716] |
| Carbohydrate Polymers | The anti-inflammatory activity of specific-sized hyaluronic acid oligosaccharides [34823813] |
| Poult Sci | Effects of different selenium sources on duodenum and jejunum tight junction network and growth performance of broilers in a model of fluorine-induced … [Pubmed:35066382] |
| Veterinary Microbiology | Self-assembled BPIV3 nanoparticles can induce comprehensive immune responses and protection against BPIV3 challenge by inducing dendritic cell maturation in … [Pubmed:35395543] |
留言咨询