C反应蛋白(CRP)检测试剂盒(酶联免疫吸附试验法,高敏型)
High Sensitive ELISA Kit for C Reactive Protein (CRP)
C-RP; PTX1; Pentraxin-Related; Pentraxin 1
- 编号HEA821Hu
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围62.5-4,000pg/mL
- 灵敏度最小可检测剂量小于等于27.4pg/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, urine, cerebrospinal fluid, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2079 ¥ 2970 ¥ 13365 ¥ 25245 ¥ 207900
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测C反应蛋白(CRP)检测试剂盒(酶联免疫吸附试验法,高敏型)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的C反应蛋白(CRP)检测试剂盒(酶联免疫吸附试验法,高敏型)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 78-92 | 88 |
| EDTA plasma(n=5) | 84-101 | 88 |
| heparin plasma(n=5) | 79-96 | 83 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的C反应蛋白(CRP)检测试剂盒(酶联免疫吸附试验法,高敏型)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中C反应蛋白(CRP)检测试剂盒(酶联免疫吸附试验法,高敏型)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 96-105% | 81-93% | 85-99% | 86-101% |
| EDTA plasma(n=5) | 84-92% | 78-103% | 97-104% | 93-105% |
| heparin plasma(n=5) | 92-99% | 91-98% | 79-90% | 86-104% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将C反应蛋白(CRP)检测试剂盒(酶联免疫吸附试验法,高敏型)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的C反应蛋白(CRP)检测试剂盒(酶联免疫吸附试验法,高敏型)与连接于固相载体上的抗体结合，然后加入生物素化的C反应蛋白(CRP)检测试剂盒(酶联免疫吸附试验法,高敏型)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的C反应蛋白(CRP)检测试剂盒(酶联免疫吸附试验法,高敏型)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| APA821Hu01 | C反应蛋白(CRP)活性蛋白 | Cell culture; Activity Assays. |
| NPA821Hu02 | C反应蛋白(CRP)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| NPA821Hu01 | C反应蛋白(CRP)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA821Hu01 | C反应蛋白(CRP)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA821Hu02 | C反应蛋白(CRP)活性蛋白 | Cell culture; Activity Assays. |
| APA821Hu61 | C反应蛋白(CRP)活性蛋白 | Cell culture; Activity Assays. |
| PAA821Hu02 | C反应蛋白(CRP)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA821Hu01 | C反应蛋白(CRP)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA821Hu71 | C反应蛋白(CRP)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| RAA821Hu22 | C反应蛋白(CRP)重组抗体 | WB; IHC; ICC. |
| RAA821Hu23 | C反应蛋白(CRP)重组抗体 | WB; IHC; ICC. |
| MAA821Hu29 | C反应蛋白(CRP)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA821Hu21 | C反应蛋白(CRP)单克隆抗体 | WB |
| MAA821Hu26 | C反应蛋白(CRP)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA821Hu27 | C反应蛋白(CRP)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA821Hu28 | C反应蛋白(CRP)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA821Hu24 | C反应蛋白(CRP)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA821Hu25 | C反应蛋白(CRP)单克隆抗体 | WB; IHC; ICC; IP. |
| RAA821Hu25 | C反应蛋白(CRP)重组抗体 | WB; IF; ICC; IHC; IP; FCM. |
| RAA821Hu21 | C反应蛋白(CRP)重组抗体 | WB; IHC; ICC. |
| MAA821Hu23 | C反应蛋白(CRP)单克隆抗体 | WB |
| MAA821Hu22 | C反应蛋白(CRP)单克隆抗体 | WB; IHC |
| FAA821Hu08 | 抗C反应蛋白(CRP)单克隆抗体 | Flow cytometry. |
| FAA821Hu02 | 抗C反应蛋白(CRP)单克隆抗体 | Flow cytometry. |
| FAA821Hu06 | 抗C反应蛋白(CRP)单克隆抗体 | Flow cytometry. |
| FAA821Hu04 | 抗C反应蛋白(CRP)单克隆抗体 | Flow cytometry. |
| LAA821Hu72 | C反应蛋白(CRP)单克隆抗体(生物素标记) | WB; IHC; ICC. |
| HEA821Hu | C反应蛋白(CRP)检测试剂盒(酶联免疫吸附试验法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA821Hu | C反应蛋白(CRP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| AEA821Hu | 抗C反应蛋白抗体(Anti-CRP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| SCA821Hu | C反应蛋白(CRP)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA821Hu | C反应蛋白(CRP)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| PSA821Hu01 | C反应蛋白(CRP)抗体对 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA821Hu01 | C反应蛋白(CRP)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
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| Early label-free analysis of mitochondrial redox states by Raman spectroscopy predicts septic outcomes [] | |
| МАРКЕРЫ КОСТНОГО РЕМОДЕЛИРОВАНИЯ ПРИ ЗАМЕЩЕНИИ ДЕФЕКТАТРАБЕКУЛЯРНОЙ КОСТНОЙ ТКАНИ РЕЗОРБИРУЕМЫМИ И … [] | |
| J Transl Med | Multi-omic signatures of atherogenic dyslipidaemia: pre-clinical target identification and validation in humans [33407555] |
| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Use of Fourier-Transform Infrared Spectroscopy (FT-IR) for Monitoring Experimental Helicobacter pylori Infection and Related Inflammatory Response in Guinea Pig?¡ [33396581] |
| Sci Rep | Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats [33833309] |
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| Biomed Pharmacother | Intravenous ferric carboxymaltose does not provide benefits in reperfused acute myocardial infarction in the rat with normal iron status [34243097] |
| Aging | Pim-2 kinase inhibits inflammation by suppressing the mTORC1 pathway in atherosclerosis [34547720] |
| Analytical and Bioanalytical Chemistry | Nets of biotin-derived gold nanoparticles as a label for the C-reactive protein immunoassay [34559243] |
| J Oleo Sci | Cardio-protective and Anti-atherosclerosis Effect of Crocetin on Vitamin D3 and HFD-induced Atherosclerosis in Rats [34615830] |
| Medicine | Early-stage serum Stanniocalcin 1 as a predictor of outcome in patients with aneurysmal subarachnoid hemorrhage [Pubmed:34941085] |
| Talanta | A rapid fluorescent aptasensor for point-of-care detection of C-reactive protein [Pubmed:35714415] |
| Experimental and Therapeutic Medicine | Construction of a rabbit model with vinorelbine administration via peripherally inserted central catheter and dynamic monitoring of changes in phlebitis and thrombosis [Pubmed:35126715] |
| Sensors and Actuators B: Chemical | A Two-step Competition Assay for Visual, Sensitive and Quantitative C-reactive Protein Detection in Low-cost Microfluidic Particle Accumulators [] |
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