表皮生长因子受体(EGFR)检测试剂盒(酶联免疫吸附试验法,高敏型)
High Sensitive ELISA Kit for Epidermal Growth Factor Receptor (EGFR)
ErbB-1; ErbB1; ER1; ERB-B1; MENA; HER1; Erythroblastic Leukemia Viral(v-Erb-B)oncogene Homolog,Avian; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
- 编号HEA757Hu
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围0.156-10ng/mL
- 灵敏度最小可检测剂量小于等于0.055ng/mL.
- 样本类型serum, plasma, tissue homogenatesand other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2911 ¥ 4158 ¥ 18711 ¥ 35343 ¥ 291060
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测表皮生长因子受体(EGFR)检测试剂盒(酶联免疫吸附试验法,高敏型)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的表皮生长因子受体(EGFR)检测试剂盒(酶联免疫吸附试验法,高敏型)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 97-104 | 101 |
| EDTA plasma(n=5) | 93-101 | 97 |
| heparin plasma(n=5) | 94-103 | 99 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的表皮生长因子受体(EGFR)检测试剂盒(酶联免疫吸附试验法,高敏型)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中表皮生长因子受体(EGFR)检测试剂盒(酶联免疫吸附试验法,高敏型)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 82-95% | 93-102% | 78-96% | 98-105% |
| EDTA plasma(n=5) | 98-105% | 94-101% | 84-102% | 88-102% |
| heparin plasma(n=5) | 92-101% | 88-105% | 79-102% | 91-102% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将表皮生长因子受体(EGFR)检测试剂盒(酶联免疫吸附试验法,高敏型)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的表皮生长因子受体(EGFR)检测试剂盒(酶联免疫吸附试验法,高敏型)与连接于固相载体上的抗体结合，然后加入生物素化的表皮生长因子受体(EGFR)检测试剂盒(酶联免疫吸附试验法,高敏型)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的表皮生长因子受体(EGFR)检测试剂盒(酶联免疫吸附试验法,高敏型)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| APA757Hu62 | 表皮生长因子受体(EGFR)活性蛋白 | Cell culture; Activity Assays. |
| RPA757Hu01 | 表皮生长因子受体(EGFR)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA757Hu61 | 表皮生长因子受体(EGFR)活性蛋白 | Cell culture; Activity Assays. |
| EPA757Hu62 | 表皮生长因子受体(EGFR)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA757Hu02 | 表皮生长因子受体(EGFR)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA757Hu61 | 表皮生长因子受体(EGFR)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA757Hu06 | 表皮生长因子受体(EGFR)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA757Hu02 | 表皮生长因子受体(EGFR)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA757Hu01 | 表皮生长因子受体(EGFR)多克隆抗体 | WB; IHC; ICC; IP. |
| FAA757Hu51 | 抗表皮生长因子受体(EGFR)多克隆抗体(别藻蓝蛋白标记) | FCM |
| MAA757Hu27 | 表皮生长因子受体(EGFR)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA757Hu29 | 表皮生长因子受体(EGFR)单克隆抗体 | WB; IHC; ICC; IP. |
| RAA757Hu21 | 表皮生长因子受体(EGFR)重组抗体 | WB; IF; ICC; IHC; IP; FCM. |
| MAA757Hu21 | 表皮生长因子受体(EGFR)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA757Hu23 | 表皮生长因子受体(EGFR)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA757Hu24 | 表皮生长因子受体(EGFR)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA757Hu25 | 表皮生长因子受体(EGFR)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA757Hu22 | 表皮生长因子受体(EGFR)单克隆抗体 | WB; IHC |
| SEA757Hu | 表皮生长因子受体(EGFR)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA757Hu | 表皮生长因子受体(EGFR)检测试剂盒(酶联免疫吸附试验法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| AEA757Hu | 抗表皮生长因子受体抗体(Anti-EGFR)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| LMA757Hu | 表皮生长因子受体(EGFR)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
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| Mol Cancer Ther | GC1118, an anti-EGFR antibody with a distinct binding epitope and superior inhibitory activity against high-affinity EGFR ligands [PubMed: 26586721] |
| Cell Death Dis. | LINC01225 promotes occurrence and metastasis of hepatocellular carcinoma in an epidermal growth factor receptor-dependent pathway [Pubmed:26938303] |
| analytica chimica acta | Microfluidic immunosensor based on mesoporous silica platform and CMK-3/poly-acrylamide-co-methacrylate of dihydrolipoic acid modified gold electrode for cancer biomarker detection. [pubmed:28335979] |
| Talanta | EGFR detection in extracellular vesicles of breast cancer patients through immunosensor based on silica-chitosan nanoplatform [Doi: 10.1016/j.talanta.2018.10.016] |
| Bioorganic Chemistry | Design, synthesis, molecular docking and biological activity evaluation of some novel indole derivatives as potent anticancer active agents and apoptosis inducers [Pubmed: 30665034] |
| Journal of enzyme inhibition and medicinal chemistry | Novel thienopyrimidine derivatives as dual EGFR and VEGFR-2 inhibitors: design, synthesis, anticancer activity and effect on cell cycle profile [Pubmed: 30919701] |
| Bioorganic Chemistry | Design and synthesis of new benzoxazole/benzothiazole-phthalimide hybrids as antitumor-apoptotic agents [] |
| Bioorganic Chemistry | Synthesis and anticancer activity of bis-benzo [d][1, 3] dioxol-5-yl thiourea derivatives with molecular docking study [Pubmed: 31288134] |
| Oxidative Medicine and Cellular Longevity | Evaluation of the Effect Derived from Silybin with Vitamin D and Vitamin E Administration on Clinical, Metabolic, Endothelial Dysfunction, Oxidative Stress Parameters … [Pubmed: 31737175] |
| Neoplasia | Patient-derived xenograft (PDX) models of colorectal carcinoma (CRC) as a platform for chemosensitivity and biomarker analysis in personalized medicine [Pubmed: 33212364] |
| Association between Serum Epidermal Growth Factor Receptor and Cyclooxygenase-2 Levels in Patients with Non-small Cell Carcinoma of Lung [] | |
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| IMMUNOLOGIC RESEARCH | Humoral immune response to epidermal growth factor receptor in lung cancer [33495907] |
| European Journal of Medicinal Chemistry | Pyrazolo[3,4-d]pyrimidine-based dual EGFR T790M/HER2 inhibitors: Design, synthesis, structure¨Cactivity relationship and biological activity as potential antitumor and anticonvulsant agents [33545637] |
| Journal of Molecular Structure | Design, synthesis, antiproliferative evaluation, and molecular docking study of new quinoxaline derivatives as apoptotic inducers and EGFR inhibitors [] |
| ACS Appl Mater Interfaces | Emitter–Quencher Pair of Single Atomic Co Sites and Monolayer Titanium Carbide MXenes for Luminol Chemiluminescent Reactions [34914377] |
| Bioorganic Chemistry | Discovery of new pyrimido [5, 4-c] quinolines as potential antiproliferative agents with multitarget actions: Rapid synthesis, docking, and ADME studies [Pubmed:35219045] |
