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表皮生长因子受体(EGFR)检测试剂盒(酶联免疫吸附试验法,高敏型)

High Sensitive ELISA Kit for Epidermal Growth Factor Receptor (EGFR)

ErbB-1; ErbB1; ER1; ERB-B1; MENA; HER1; Erythroblastic Leukemia Viral(v-Erb-B)oncogene Homolog,Avian; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

  • 表皮生长因子受体(EGFR)检测试剂盒(酶联免疫吸附试验法,高敏型) 产品包装(模拟)
  • 表皮生长因子受体(EGFR)检测试剂盒(酶联免疫吸附试验法,高敏型) 产品包装(模拟)
  • 表皮生长因子受体(EGFR)检测试剂盒(酶联免疫吸附试验法,高敏型) 实验结果图
  • HEA757Hu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测表皮生长因子受体(EGFR)检测试剂盒(酶联免疫吸附试验法,高敏型),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的表皮生长因子受体(EGFR)检测试剂盒(酶联免疫吸附试验法,高敏型)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 97-104 101
EDTA plasma(n=5) 93-101 97
heparin plasma(n=5) 94-103 99

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的表皮生长因子受体(EGFR)检测试剂盒(酶联免疫吸附试验法,高敏型),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中表皮生长因子受体(EGFR)检测试剂盒(酶联免疫吸附试验法,高敏型)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 82-95% 93-102% 78-96% 98-105%
EDTA plasma(n=5) 98-105% 94-101% 84-102% 88-102%
heparin plasma(n=5) 92-101% 88-105% 79-102% 91-102%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将表皮生长因子受体(EGFR)检测试剂盒(酶联免疫吸附试验法,高敏型)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的表皮生长因子受体(EGFR)检测试剂盒(酶联免疫吸附试验法,高敏型)与连接于固相载体上的抗体结合,然后加入生物素化的表皮生长因子受体(EGFR)检测试剂盒(酶联免疫吸附试验法,高敏型)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的表皮生长因子受体(EGFR)检测试剂盒(酶联免疫吸附试验法,高敏型)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APA757Hu62 表皮生长因子受体(EGFR)活性蛋白 Cell culture; Activity Assays.
RPA757Hu01 表皮生长因子受体(EGFR)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA757Hu61 表皮生长因子受体(EGFR)活性蛋白 Cell culture; Activity Assays.
EPA757Hu62 表皮生长因子受体(EGFR)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA757Hu02 表皮生长因子受体(EGFR)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPA757Hu61 表皮生长因子受体(EGFR)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA757Hu06 表皮生长因子受体(EGFR)多克隆抗体 WB; IHC; ICC; IP.
PAA757Hu02 表皮生长因子受体(EGFR)多克隆抗体 WB; IHC; ICC; IP.
PAA757Hu01 表皮生长因子受体(EGFR)多克隆抗体 WB; IHC; ICC; IP.
FAA757Hu51 抗表皮生长因子受体(EGFR)多克隆抗体(别藻蓝蛋白标记) FCM
MAA757Hu27 表皮生长因子受体(EGFR)单克隆抗体 WB; IHC; ICC; IP.
MAA757Hu29 表皮生长因子受体(EGFR)单克隆抗体 WB; IHC; ICC; IP.
RAA757Hu21 表皮生长因子受体(EGFR)重组抗体 WB; IF; ICC; IHC; IP; FCM.
MAA757Hu21 表皮生长因子受体(EGFR)单克隆抗体 WB; IHC; ICC; IP.
MAA757Hu23 表皮生长因子受体(EGFR)单克隆抗体 WB; IHC; ICC; IP.
MAA757Hu24 表皮生长因子受体(EGFR)单克隆抗体 WB; IHC; ICC; IP.
MAA757Hu25 表皮生长因子受体(EGFR)单克隆抗体 WB; IHC; ICC; IP.
MAA757Hu22 表皮生长因子受体(EGFR)单克隆抗体 WB; IHC
SEA757Hu 表皮生长因子受体(EGFR)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA757Hu 表皮生长因子受体(EGFR)检测试剂盒(酶联免疫吸附试验法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
AEA757Hu 抗表皮生长因子受体抗体(Anti-EGFR)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antibody Detection.
LMA757Hu 表皮生长因子受体(EGFR)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Thoracic Cancer Nimotuzumab combined with gemcitabine and cisplatin as second-line chemotherapy for advanced non-small-cell lung cancer [Wiley: source]
J Cent Nerv Syst Dis.? Increased Epidermal Growth Factor Receptor (EGFR) Associated with Hepatocyte Growth Factor (HGF) and Symptom Severity in Children with Autism … [Pubmed:25249767]
Mol Cancer Ther GC1118, an anti-EGFR antibody with a distinct binding epitope and superior inhibitory activity against high-affinity EGFR ligands [PubMed: 26586721]
Cell Death Dis.  LINC01225 promotes occurrence and metastasis of hepatocellular carcinoma in an epidermal growth factor receptor-dependent pathway [Pubmed:26938303]
analytica chimica acta Microfluidic immunosensor based on mesoporous silica platform and CMK-3/poly-acrylamide-co-methacrylate of dihydrolipoic acid modified gold electrode for cancer biomarker detection. [pubmed:28335979]
Talanta EGFR detection in extracellular vesicles of breast cancer patients through immunosensor based on silica-chitosan nanoplatform [Doi: 10.1016/j.talanta.2018.10.016]
Bioorganic Chemistry Design, synthesis, molecular docking and biological activity evaluation of some novel indole derivatives as potent anticancer active agents and apoptosis inducers [Pubmed: 30665034]
Journal of enzyme inhibition and medicinal chemistry Novel thienopyrimidine derivatives as dual EGFR and VEGFR-2 inhibitors: design, synthesis, anticancer activity and effect on cell cycle profile [Pubmed: 30919701]
Bioorganic Chemistry Design and synthesis of new benzoxazole/benzothiazole-phthalimide hybrids as antitumor-apoptotic agents []
Bioorganic Chemistry Synthesis and anticancer activity of bis-benzo [d][1, 3] dioxol-5-yl thiourea derivatives with molecular docking study [Pubmed: 31288134]
Oxidative Medicine and Cellular Longevity Evaluation of the Effect Derived from Silybin with Vitamin D and Vitamin E Administration on Clinical, Metabolic, Endothelial Dysfunction, Oxidative Stress Parameters … [Pubmed: 31737175]
Neoplasia Patient-derived xenograft (PDX) models of colorectal carcinoma (CRC) as a platform for chemosensitivity and biomarker analysis in personalized medicine [Pubmed: 33212364]
Association between Serum Epidermal Growth Factor Receptor and Cyclooxygenase-2 Levels in Patients with Non-small Cell Carcinoma of Lung []
Synthesis, EGFR-TK inhibition and anticancer activity of new quinoxaline derivatives []
IMMUNOLOGIC RESEARCH Humoral immune response to epidermal growth factor receptor in lung cancer [33495907]
European Journal of Medicinal Chemistry Pyrazolo[3,4-d]pyrimidine-based dual EGFR T790M/HER2 inhibitors: Design, synthesis, structure¨Cactivity relationship and biological activity as potential antitumor and anticonvulsant agents [33545637]
Journal of Molecular Structure Design, synthesis, antiproliferative evaluation, and molecular docking study of new quinoxaline derivatives as apoptotic inducers and EGFR inhibitors []
ACS Appl Mater Interfaces Emitter–Quencher Pair of Single Atomic Co Sites and Monolayer Titanium Carbide MXenes for Luminol Chemiluminescent Reactions [34914377]
Bioorganic Chemistry Discovery of new pyrimido [5, 4-c] quinolines as potential antiproliferative agents with multitarget actions: Rapid synthesis, docking, and ADME studies [Pubmed:35219045]
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