Ⅲ型前胶原氨基端原肽(PIIINP)检测试剂盒(酶联免疫吸附试验法,高敏型)
High Sensitive ELISA Kit for Procollagen III N-Terminal Propeptide (PIIINP)
P3NP; N-Propeptide Of Type III Procollagen; Procollagen III Amino Terminal Propeptide
- 编号HEA573Hu
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围62.5-4,000pg/mL
- 灵敏度最小可检测剂量小于等于20.6pg/mL.
- 样本类型serum, plasma and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 3108 ¥ 4440 ¥ 19980 ¥ 37740 ¥ 310800
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
-
产品包装(模拟)
-
产品包装(模拟)
-
实验结果图
-
标准曲线图
-
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测Ⅲ型前胶原氨基端原肽(PIIINP)检测试剂盒(酶联免疫吸附试验法,高敏型)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的Ⅲ型前胶原氨基端原肽(PIIINP)检测试剂盒(酶联免疫吸附试验法,高敏型)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 96-103 | 99 |
| EDTA plasma(n=5) | 96-103 | 99 |
| heparin plasma(n=5) | 97-104 | 101 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的Ⅲ型前胶原氨基端原肽(PIIINP)检测试剂盒(酶联免疫吸附试验法,高敏型)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中Ⅲ型前胶原氨基端原肽(PIIINP)检测试剂盒(酶联免疫吸附试验法,高敏型)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 95-104% | 88-98% | 90-98% | 82-91% |
| EDTA plasma(n=5) | 94-102% | 78-96% | 87-104% | 91-105% |
| heparin plasma(n=5) | 82-99% | 85-101% | 85-104% | 98-105% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将Ⅲ型前胶原氨基端原肽(PIIINP)检测试剂盒(酶联免疫吸附试验法,高敏型)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的Ⅲ型前胶原氨基端原肽(PIIINP)检测试剂盒(酶联免疫吸附试验法,高敏型)与连接于固相载体上的抗体结合，然后加入生物素化的Ⅲ型前胶原氨基端原肽(PIIINP)检测试剂盒(酶联免疫吸附试验法,高敏型)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的Ⅲ型前胶原氨基端原肽(PIIINP)检测试剂盒(酶联免疫吸附试验法,高敏型)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA573Hu02 | Ⅲ型前胶原氨基端原肽(PIIINP)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA573Hu62 | Ⅲ型前胶原氨基端原肽(PIIINP)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA573Hu63 | Ⅲ型前胶原氨基端原肽(PIIINP)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA573Hu01 | Ⅲ型前胶原氨基端原肽(PIIINP)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA573Hu61 | Ⅲ型前胶原氨基端原肽(PIIINP)活性蛋白 | Cell culture; Activity Assays. |
| EPA573Hu61 | Ⅲ型前胶原氨基端原肽(PIIINP)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPA573Hu21 | Ⅲ型前胶原氨基端原肽(PIIINP)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| SPA573Hu02 | Ⅲ型前胶原氨基端原肽(PIIINP)合成多肽 | Immunogen; Blocking Peptide. |
| CPA573Hu22 | Ⅲ型前胶原氨基端原肽(PIIINP)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| CPA573Hu31 | Ⅲ型前胶原氨基端原肽(PIIINP)钥孔血蓝蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| SPA573Hu01 | Ⅲ型前胶原氨基端原肽(PIIINP)合成多肽 | Immunogen; Blocking Peptide. |
| SPA573Hu71 | Ⅲ型前胶原氨基端原肽(PIIINP)合成多肽 | Immunogen; Blocking Peptide. |
| PAA573Hu01 | Ⅲ型前胶原氨基端原肽(PIIINP)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA573Hu02 | Ⅲ型前胶原氨基端原肽(PIIINP)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA573Hu09 | Ⅲ型前胶原氨基端原肽(PIIINP)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA573Hu08 | Ⅲ型前胶原氨基端原肽(PIIINP)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA573Hu71 | Ⅲ型前胶原氨基端原肽(PIIINP)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAA573Hu23 | Ⅲ型前胶原氨基端原肽(PIIINP)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA573Hu24 | Ⅲ型前胶原氨基端原肽(PIIINP)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA573Hu27 | Ⅲ型前胶原氨基端原肽(PIIINP)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA573Hu28 | Ⅲ型前胶原氨基端原肽(PIIINP)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA573Hu26 | Ⅲ型前胶原氨基端原肽(PIIINP)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA573Hu29 | Ⅲ型前胶原氨基端原肽(PIIINP)单克隆抗体 | WB |
| MAA573Hu25 | Ⅲ型前胶原氨基端原肽(PIIINP)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA573Hu21 | Ⅲ型前胶原氨基端原肽(PIIINP)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA573Hu22 | Ⅲ型前胶原氨基端原肽(PIIINP)单克隆抗体 | WB; IHC; ICC; IP. |
| FAA573Hu04 | 抗Ⅲ型前胶原氨基端原肽(PIIINP)单克隆抗体 | Flow cytometry. |
| FAA573Hu08 | 抗Ⅲ型前胶原氨基端原肽(PIIINP)单克隆抗体 | Flow cytometry. |
| FAA573Hu06 | 抗Ⅲ型前胶原氨基端原肽(PIIINP)单克隆抗体 | Flow cytometry. |
| FAA573Hu02 | 抗Ⅲ型前胶原氨基端原肽(PIIINP)单克隆抗体 | Flow cytometry. |
| LAA573Hu94 | Ⅲ型前胶原氨基端原肽(PIIINP)单克隆抗体(辣根过氧化物酶标记) | IHC; ICC |
| HEA573Hu | Ⅲ型前胶原氨基端原肽(PIIINP)检测试剂盒(酶联免疫吸附试验法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA573Hu | Ⅲ型前胶原氨基端原肽(PIIINP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA573Hu | Ⅲ型前胶原氨基端原肽(PIIINP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA573Hu | Ⅲ型前胶原氨基端原肽(PIIINP)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| PSA573Hu01 | Ⅲ型前胶原氨基端原肽(PIIINP)抗体对 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA573Hu11 | Ⅲ型前胶原氨基端原肽(PIIINP)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
| KSA573Hu01 | Ⅲ型前胶原氨基端原肽(PIIINP)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
| 杂志 | 参考文献 |
| Molecular Biology Reports | Characterization of the specific and sustained GH1 expression induced by rAAV2/1 in normal adult male rats [PubMed: 20204528] |
| Obesity Surgery | A Biomarker Panel for Non-alcoholic Steatohepatitis (NASH) and NASH-Related Fibrosis [PubMed: 20532833] |
| Liver Biopsy | Comparison of Five Liver Fibrosis Indexes with Serum Levels of Laminin and N Terminal Peptide of Procollagen Type III in Chronic Hepatitis Patients [Intechopen: 18785] |
| Trials | Is there benefit in optimising heart failure treatment in over-80 year-old patients? (HF-80 study): study protocol for a randomized controlled trial. [Trials: 1745-6215] |
| American Journal of Hypertension | Aliskiren and Perindopril Reduce the Levels of Transforming Growth Factor-β in Patients With Non-Diabetic Kidney Disease [Nature: 201214] |
| Arthritis Research and Therapy | Raised circulating tenascin-C in rheumatoid arthritis. [PubMed: 23193984] |
| American Heart Journal | Serum markers of deranged myocardial collagen turnover: their relation to malignant ventricular arrhythmias in cardioverter-defibrillator recipients with heart failure. [PubMed: 23067911] |
| hepatology research | Diagnostic potential of serum direct markers and non-invasive fibrosis models in patients with chronic hepatitis B. [PubMed: 22734888] |
| Digestive and Liver Disease | Effects of coffee consumption in chronic hepatitis C: a randomized controlled trial [Pubmed: 23238034] |
| Journal of Infectious Diseases | Procollagen III N-terminal propeptide and desmosine are released by matrix destruction in pulmonary tuberculosis [Pubmed: 23922364] |
| International Urogynecology Journal | Prevalence, etiology and risk factors of pelvic organ prolapse in premenopausal primiparous women [Springer: Source] |
| Journal of Clinical and Experimental Hepatology | Protective Effects of Norursodeoxycholic Acid Versus Ursodeoxycholic Acid on Thioacetamide-induced Rat Liver Fibrosis [ScienceDirect: S0973688314000073] |
| Der Urologe | Retroperitoneale Fibrose [Springer:Source] |
| Ultrasound in Obstetrics & Gynecology | The status of the pelvic floor in young primiparous women [Wiley:Source] |
| PLoS One. | Clinical significance of markers of collagen metabolism in rheumatic mitral valve disease [Pubmed:24603967] |
| Clin Chim Acta. | Serum and urinary procollagen III aminoterminal propeptide as a biomarker of obstructive nephropathy in children [Pubmed:24768785] |
| BMC Gastroenterol. | Fast food diet with CCl4 micro-dose induced hepatic-fibrosis-a novel animal model [Pubmed:24884574] |
| Toxicology. | DNMT3A silencing RASSF1A promotes cardiac fibrosis through upregulation of ERK1/2 [Pubmed:24945829] |
| Southeast Asian J Trop Med Public Health. | Correlation of HBsAg titers with serum fibrotic maker in patients with chronic hepatitis B infection. [Pubmed:24974647] |
| The American Journal of Cardiology | Relation of burden of myocardial fibrosis to malignant ventricular arrhythmias and outcomes in Fabry disease [Pubmed:25073565] |
| Ann Rheum Dis. | B-cell depletion attenuates serological biomarkers of fibrosis and myofibroblast activation in IgG4-related disease [Pubmed:25143523] |
| Scientific Reports | Biomarkers and Echocardiographic Predictors of Myocardial Dysfunction in Patients with Hypertension [PubMed: 25747153] |
| Int J Mol Sci | The Multi-Biomarker Approach for Heart Failure in Patients with Hypertension [PubMed: 25984599] |
| Viral Hepatitis Journal | The Relationship of the Degree of Hepatic Fibrosis with Hyaluronic Acid, Type 4 Collagen, and Procollagen Type 3 N-Terminal Peptide Levels in Patients with Chronic Viral Hepatitis [Openview: 8A0B8C97D72C61741371F33508760B75] |
| International Journal of Experimental Pathology | Pathological characterization and morphometric analysis of hepatic lesions in SHRSP5/Dmcr, an experimental non-alcoholic steatohepatitis model, induced by high-fat and high-cholesterol diet [doi:10.1111] |
| Heart Rhythm. | Prognostic value of collagen turnover biomarkers in cardiac resynchronization therapy: A subanalysis of the TRUST CRT randomized trial population [Pubmed:26776557] |
| BMJ Open Sport Exerc Med | Procollagen markers in microdialysate can predict patient outcome after Achilles tendon rupture [content:2] |
| Inflammation Research | Fibrosis of extracellular matrix is related to the duration of the disease but is unrelated to the dynamics of collagen metabolism in dilated cardiomyopathy [pubmed:27516211] |
| Heart & Vessels | Left ventricular reverse remodeling is not related to biopsy-detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR [pubmed:28004175] |
| american journal of physiology-renal physiology | Extracellular microRNA signature in chronic kidney disease. [pubmed:28077372] |
| Cellular Physiology and Biochemistry | Renal Denervation Attenuates Multi-Organ Fibrosis and Improves Vascular Remodeling in Rats with Transverse Aortic Constriction Induced Cardiomyopathy [pubmed:27889753] |
| PLoS One. | Procollagen C-Proteinase Enhancer 1 (PCPE-1) as a Plasma Marker of Muscleand Liver Fibrosis in Mice. [pubmed:27458976] |
| International Journal of Cardiology | 5-methoxytryptophan is a potential marker for post-myocardial infarction heart failure - apreliminary approach to clinical utility. [pubmed:27526355] |
| Transplant Proc | Urinary Type III Procollagen Is Associated With Chronic Allograft Dysfunction and Predicts Graft Survival. [pubmed:28219585] |
| Knee Surg Sports Traumatol Arthrosc | Achilles tendon rupture healing is enhanced by intermittent pneumatic compression upregulating collagen type I synthesis [pubmed:28668970] |
| Antiviral Therapy | Association of HIV infection with biomarkers of kidney injury and fibrosis in the Multicenter AIDS Cohort Study [pubmed:28054933] |
| Molecular Medicine Reports | Profilin‑1 contributes to cardiac injury induced by advanced glycation end‑products in rats [pubmed:28901418] |
| Biomarkers Medicial | Relationship of biomarkers of extracellular matrix with myocardial function in Type 2 diabetes mellitus [pubmed:28685602] |
| Heart and Vessels | Left ventricular reverse remodeling is not related to biopsy‑detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR [10.1007/s00380-016-0930-y] |
| Clinical Infectious Diseases | Matrix Degradation in Human Immunodeficiency Virus Type 1–Associated Tuberculosis and Tuberculosis Immune Reconstitution Inflammatory Syndrome: A Prospective Observational Study [10.1093/cid/cix231] |
| Cytokine | 12-month patterns of serum markers of collagen synthesis, transforming growth factor and connective tissue growth factor are similar in new-onset and chronic dilated cardiomyopathy in patients both with and without cardiac fibrosis. [pubmed:28460256] |
| Advanced Healthcare Materials | Prognostic value of fibrosis-related markers in dilated cardiomyopathy: A link between osteopontin and cardiovascular events. [pubmed:29120858] |
| American Journal of Kidney Diseases | Urinary Biomarkers of Kidney Tubular Damage and Risk of Cardiovascular Disease and Mortality in Elders [Pubmed:29602632] |
| Angiology | The Association of Biomarkers of Inflammation and Extracellular Matrix Degradation With the Risk of Abdominal Aortic Aneurysm: The ARIC Study [article:10.1007] |
| Hernia | Circulating matrix metalloproteinases and procollagen propeptides in inguinal hernia [Pubmed:29484522] |
| Cardiovascular Journal of Africa | Assessment of myocardial fibrosis by late gadolinium enhancement imaging and biomarkers of collagen metabolism in chronic rheumatic mitral regurgitation. [Pubmed:29443354] |
| Turk Kardiyol Dern Ars. | Association between reverse electrical remodeling and cardiac fibrosis markers in patients with cardiac resynchronization therapy [Pubmed:29512624] |
| Кардиоваскулярная терапия и профилактика | N-терминальный пропептид проколлагена III типа в качестве возможного сывороточного маркера фиброза миокарда у больных сахарным диабетом 2 … [:] |
| Clinical Infectious Diseases | Improvement in Hepatic Fibrosis Biomarkers Associated with Chemokine Receptor Inactivation through Mutation or Therapeutic Blockade [Pubmed: 30239650] |
| Anatolian Journal of Cardiology | microRNA-29a inhibits cardiac fibrosis in Sprague-Dawley rats by downregulating the expression of DNMT3A. [Pubmed: 30297596] |
| Contemporary Clinical Trials | Methods to improve the noninvasive diagnosis and assessment of disease severity in children with suspected nonalcoholic fatty liver disease (NAFLD): Study design [Doi: 10.1016/j.cct.2018.10.012] |
| Arthritis Research & Therapy | Association of serum levels of fibrosis-related biomarkers with disease activity in patients with IgG4-related disease [Pubmed: 30547825] |
| American Journal of Respiratory and Critical Care Medicine | Identification and Modulation of Microenvironment is Crucial for Effective MSC Therapy in Acute Lung Injury [Pubmed: 30521764] |
| Direktor: Prof. Dr. med. Andreas Mügge [] | |
| Scientific Reports | Low fibrosis biomarker levels predict cardiac resynchronization therapy response [Pubmed: 30988339] |
| American Journal of Translational Research | Development and validation of an in vitro 3D model of NASH with severe fibrotic phenotype [Pubmed: 30972180] |
| Food and Chemical Toxicology | Gum Acacia mitigates diclofenac nephrotoxicity by targeting monocyte chemoattractant protein-1, complement receptor-1 and pro-apoptotic pathways [Pubmed: 31042592] |
| Digestive diseases and sciences | Validation and Refinement of Noninvasive Methods to Assess Hepatic Fibrosis: Magnetic Resonance Elastography Versus Enhanced Liver Fibrosis Index [Pubmed: 31468264] |
| Journal of Diabetes Research | Type 2 Diabetes Mellitus and Chronic Heart Failure with Midrange and Preserved Ejection Fraction: A Focus on Serum Biomarkers of Fibrosis [Pubmed: 33224989] |
| BMC GASTROENTEROLOGY | Changes in hepatic fibrosis and vitamin D levels after viral hepatitis C eradication using direct-acting antiviral therapy [Pubmed: 33069226] |
| european heart journal | A randomized controlled trial of dapagliflozin on left ventricular hypertrophy in people with type two diabetes: the DAPA-LVH trial [Pubmed: 32578850] |
| Ассоциации толщины эпикардиального жира и циркулирующих маркеров фиброза миокарда у пациентов с инфарктом миокарда [] | |
| J Am Geriatr Soc | Association of Non©\Steroidal Anti©\Inflammatory Drugs with Kidney Health in Ambulatory Older Adults [33305369] |
| Aging (Albany NY) | Prognostic potential of cardiac structural and functional parameters and N-terminal propeptide of type III procollagen in predicting cardiac fibrosis one year after?¡ [33431713] |
| PeerJ | Effect of vitamin D supplementation in patients with chronic hepatitis C after direct-acting antiviral treatment: a randomized, double-blind, placebo-controlled trial [33614272] |
| Open Forum Infectious Diseases | Soluble CD163 Identifies Those at Risk for Increased Hepatic Inflammation & Fibrosis [34104667] |
| European Heart Journal | A randomized controlled trial of dapagliflozin on left ventricular hypertrophy in people with type two diabetes [32578850] |
| Nutrients | The Effect of Daily Methylsulfonylmethane (MSM) Consumption on High-Density Lipoprotein Cholesterol in Healthy Overweight and Obese Adults: A Randomized … [34684621] |
| J Diabetes Res | Association between Markers of Fibrosis and Heart Failure Incidence in Patients with Type 2 Diabetes Mellitus [34778465] |
| Scandinavian Cardiovascular Journal | Dual integrin αvβ3 and αvβ5 blockade attenuates cardiac dysfunction by reducing fibrosis in a rat model of doxorubicin-induced cardiomyopathy [34296634] |
| J. Clin. Med | Simple Predictors for Cardiac Fibrosis in Patients with Type 2 Diabetes Mellitus: The Role of Circulating Biomarkers and Pulse Wave Velocity [Pubmed:35628969] |
| Biomolecules | Increase in Serum MMP-9 and TIMP-1 Concentrations during Alcohol Intoxication in Adolescents—A Preliminary Study [Pubmed:35625637] |
留言咨询