干扰素β(IFNb)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Interferon Beta (IFNb) ,etc. by FLIA (Flow Luminescence Immunoassay)
IFNB1; IFN-B; IFB; IFF; IFNB; Interferon Beta 1 Fibroblast
（注：单次混测多因子不超过8个指标 ）
- 编号LMA222Hu
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3.5h
- 检测范围0.98-1000pg/mL
- 灵敏度最小可检测剂量小于等于0.327 pg/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
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产品包装(模拟)
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产品包装(模拟)
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测干扰素β(IFNb)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的干扰素β(IFNb)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 81-105 | 101 |
| EDTA plasma(n=5) | 97-104 | 101 |
| heparin plasma(n=5) | 94-103 | 97 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的干扰素β(IFNb)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中干扰素β(IFNb)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 95-103% | 79-89% | 90-101% | 92-99% |
| EDTA plasma(n=5) | 92-101% | 87-96% | 92-101% | 80-105% |
| heparin plasma(n=5) | 83-92% | 90-99% | 93-105% | 85-99% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。
实验原理
将干扰素β(IFNb)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的干扰素β(IFNb)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的干扰素β(IFNb)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的干扰素β(IFNb)等多因子检测试剂盒(流式荧光发光法)呈正相关。
赠品
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| APA222Hu02 | 干扰素β(IFNb)活性蛋白 | Cell culture; Activity Assays. |
| RPA222Hu02 | 干扰素β(IFNb)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA222Hu01 | 干扰素β(IFNb)活性蛋白 | Cell culture; Activity Assays. |
| RPA222Hu01 | 干扰素β(IFNb)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA222Hu01 | 干扰素β(IFNb)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA222Hu02 | 干扰素β(IFNb)多克隆抗体 | WB; IHC; ICC; IP. |
| MAA222Hu22 | 干扰素β(IFNb)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA222Hu21 | 干扰素β(IFNb)单克隆抗体 | WB,IHC |
| MAA222Hu23 | 干扰素β(IFNb)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA222Hu24 | 干扰素β(IFNb)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA222Hu25 | 干扰素β(IFNb)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA222Hu26 | 干扰素β(IFNb)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA222Hu29 | 干扰素β(IFNb)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA222Hu27 | 干扰素β(IFNb)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA222Hu | 干扰素β(IFNb)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA222Hu | 干扰素β(IFNb)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| AEA222Hu | 抗干扰素β抗体(Anti-IFNb)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| LMA222Hu | 干扰素β(IFNb)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA222Hu01 | 干扰素β(IFNb)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
| 杂志 | 参考文献 |
| 20 | Melatonin protects liver against ischemia and reperfusion injury through inhibition of toll-like receptor signaling pathway [PubMed: 21355876] |
| Journal of General Virology | Hepatitis B virus polymerase inhibits RIG-I- and Toll-like receptor 3-mediated beta interferon induction in human hepatocytes through interference with interferon regulatory factor 3 activation and dampening of the interaction between TBK1/IKKε and DDX3. [Sgm: 9182080] |
| Biochemical and Biophysical Research Communications | Impaired TLR3/IFN-β signaling in monocyte-derived dendritic cells from patients with acute-on-chronic hepatitis B liver failure: Relevance to the severity of liver damage [ScienceDirect: S0006291X09019822] |
| PLoS One | Intact Type I Interferon Production and IRF7 Function in Sooty Mangabeys [PubMed: PMC3757038] |
| PLoS Neglected Tropical Diseases | Innate Immune Response to Rift Valley Fever Virus in Goats [PubMed: PMC3335883] |
| BioMed Research International | Low pH Environmental Stress Inhibits LPS and LTA-Stimulated Proinflammatory Cytokine Production in Rat Alveolar Macrophages [Pubmed: 24288685] |
| Vet Microbiol. | An attenuated EIAV strain and its molecular clone strain differentially induce the expression of Toll-like receptors and type-I interferons in equine monocyte-derived macrophages. [Pubmed: 23850441] |
| Food and Chemical Toxicology | Scoparone attenuates d-galactosamine/lipopolysaccharide-induced fulminant hepatic failure through inhibition of toll-like receptor 4 signaling in mice [Pubmed: 23535186] |
| Veterinary immunology and immunopathology | Experimental model of equine alveolar macrophage stimulation with TLR ligands [Pubmed: 23815824] |
| American journal of respiratory cell and molecular biology | Training Modifies Innate Immune Responses in Blood Monocytes and in Pulmonary Alveolar Macrophages [Pubmed: 24502337] |
| FEBS Lett. | Mannan binding lectin attenuates double-stranded RNA-mediated TLR3 activation and innate immunity [Pubmed:24530528] |
| Veterinary Research | Infection of equine monocyte-derived macrophages with an attenuated equine infectious anemia virus (EIAV) strain induces a strong resistance to the infection by a virulent EIAV strain [Pubmed:25106750] |
| PLOS Neglected Tropical Diseases | MicroRNA-30e* Suppresses Dengue Virus Replication by Promoting NF-κB–Dependent IFN Production [Pubmed:25122182] |
| Veterinary Research | The innate immune response of equine bronchial epithelial cells is altered by training [Pubmed:25595212] |
| molecular and celluar biology | SOCS3 Drives Proteasomal Degradation of TBK1 and Negatively Regulates Antiviral Innate Immunity [PubMed: 25939384] |
| Arch Virol | Analysis of cytokine production in a newly developed canine tracheal epithelial cell line infected with H3N3 canine influenza virus [PubMed: 25797196] |
| J | Complexes of DNA with the Antimicrobial Peptide LL37 Augment NK Cell Functions by Inducing Type I Interferon Production from Circulating Monocytes and Plasmacytoid Predendritic Cells [PubMed: 26258404] |
| Virus | PKR activation enhances replication of classical swine fever virus in PK-15 cells [PubMed: 25899421] |
| PLoS One | siRNA Targeting the 2A pro Genomic Region Prevents Enterovirus 71 Replication In Vitro [Pubmed:26886455] |
| Scientific Reports | Cellular uptake of exogenous calcineurin B is dependent on TLR4/MD2/CD14 complexes, and CnB is an endogenous ligand of TLR4 [Pubmed:27090571] |
| Front Microbiol | Innate Immune Responses in ALV-J Infected Chicks and Chickens with Hemangioma In Vivo [Pubmed:27252695] |
| Canadian Journal of Physiology and Pharmacology | Chloroquine attenuates lipopolysaccharide-induced inflammatory responses through upregulation of USP25. [pubmed:28134560] |
| International Journal of Molecular Medicine | Effects of IRF1 and IFN-β interaction on the M1 polarization of macrophages and its antitumor function [pubmed:27176664] |
| International Journal of Clinical and Experimental Pathology | Host miR-146a promotes replication of human cytomegalovirus by suppressing type I IFN response in MRC-5 cells [files:ijcep0036479.pdf] |
| Poultry Science | ALV-J strain SCAU-HN06 induces innate immune responses in chicken primary monocyte-derived macrophages [pubmed:27486255] |
| Journal of Virology | Characterization of Simian Immunodeficiency Virus Variants Anatomically Compartmentalized inPlasma and Milk in Chronically Infected African Green Monkeys. [pubmed:] |
| Biochemical and Biophysical Research Communications | MicroRNA-548j inhibits type I interferon production by targeting ZBTB11 in patients with chronic hepatitis B [pubmed:28342861] |
| Virology | The down-regulation of casein kinase 1 alpha as a host defense response against infectious bursal disease virus infection [pubmed:28988058] |
| EMBO Journal | Zika virus elicits inflammation to evade antiviral response by cleaving cGAS via NS1‐caspase‐1 axis [Pubmed:30065070] |
| Toxicology and applied pharmacology | Bisphenol A induced male germ cell apoptosis via IFNβ-XAF1-XIAP pathway in adult mice [Pubmed:30017639] |
| Archives of Virology | MAVS induces a host cell defense to inhibit CSFV infection [Pubmed:29556776] |
| Journal of virology | Inhibition of DNA-sensing pathway by Marek's disease virus VP23 protein through suppression of interferon regulatory factor 7 activation [Pubmed: 30518647] |
| Veterinary Immunology and Immunopathology | Molecular cloning and functional characterization of porcine 2′, 5′-oligoadenylate synthetase 1b and its effect on infection with porcine reproductive and respiratory?… [Doi: 10.1016/j.vetimm.2019.01.003] |
| Science Signaling | HIPK2 is necessary for type I interferon–mediated antiviral immunity [Doi: 10.1126/scisignal.aau4604] |
| INTERNATIONAL MEDICAL RESEARCH | Evaluation of serum interferons in patients with age-related macular degeneration [Pubmed: 31007694] |
| Veterinary Immunology and Immunopathology | Differential expression of type I interferon mRNA and protein levels induced by virulent Marek's disease virus infection in chickens [] |
| International journal of biological macromolecules | Porcine FcγRIIb mediated PRRSV ADE infection through inhibiting IFN-β by cytoplasmic inhibitory signal transduction [Pubmed: 31284005] |
| Journal of virology | Marek's Disease Virus RLORF4 Inhibits Type I Interferon Production by Antagonizing NF-κB Activation [Pubmed: 31243133] |
| The Journal of Latvian Academy of Sciences | Serodiagnosis of Human Bocavirus 1 Infection among Hospitalised Children with Lower Respiratory Tract Infection in Latvia [] |
| Cancer Medicine | The immunostimulatory effects and pro‐apoptotic activity of rhCNB against Lewis lung cancer is mediated by Toll‐like receptor 4 [Pubmed: 31218844] |
| FASEB Journal | Scavenger receptor A impairs interferon response to HBV infection by limiting TRAF 3 ubiquitination through recruiting OTUB 1 [] |
| PLOS Pathogens | Avian oncogenic herpesvirus antagonizes the cGAS-STING DNA-sensing pathway to mediate immune evasion [Pubmed: 31539404] |
| Autophagy | Induction of autophagy and suppression of type I IFN secretion by CSFV [Pubmed: 32160078] |
| MOLECULAR IMMUNOLOGY | BVDV-1 induces interferon-beta gene expression through a pathway involving IRF1, IRF7, and NF-κB activation [Pubmed: 33053462] |
| Autophagy | LDHB inhibition induces mitophagy and facilitates the progression of CSFV infection [Pubmed: 32924761] |
| Arch Virol . | Infectious bronchitis virus inhibits activation of the TLR7 pathway, but not the TLR3 pathway [Pubmed: 32532134] |
| Virulence | Determination of antiviral action of Long Non-coding RNA loc107051710 During Infectious Bursal Disease Virus Infection due to Enhancement of Interferon … [] |
| bioRxiv | Transferrin-dependent crosstalk between the intestinal tract and commensal microbes contributes for immune tolerance [] |
| Res Vet Sci | Open reading frame 5 protein of porcine circovirus type 2 induces RNF128 (GRAIL) which inhibits mRNA transcription of interferon-β in porcine epithelial cells [34416463] |
| Eur J Immunol | Anti‐IFN‐α/‐ω neutralizing antibodies from COVID‐19 patients correlate with downregulation of IFN response and laboratory biomarkers of disease severity [Pubmed:35419822] |
| ResearchSquare | Chicken Intestinal Microbiota Modulation of Resistance to Nephropathogenic Infectious Bronchitis Virus Infection Through IFN-I [] |
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