碱性磷酸酶(ALP)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Alkaline Phosphatase (ALP) ,etc. by FLIA (Flow Luminescence Immunoassay)
AKP; ALKP; Basic Phosphatase
（注：单次混测多因子不超过8个指标 ）
- 编号LMB472Bo
- 物种Bos taurus; Bovine (Cattle，牛) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3.5h
- 检测范围0.02-20ng/mL
- 灵敏度最小可检测剂量小于等于0.007 ng/mL.
- 样本类型Tissue homogenates, cell lysates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 8指标数 7指标数 6指标数 5指标数 4指标数 3指标数 2指标数1指标数
- 价格 ¥ 2621 ¥ 2722 ¥ 2873 ¥ 3074 ¥ 3276 ¥ 3578 ¥ 4032 ¥ 5040 添加到价格计算器 计算器
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
-
产品包装(模拟)
-
产品包装(模拟)
-
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测碱性磷酸酶(ALP)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。
实验原理
将碱性磷酸酶(ALP)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的碱性磷酸酶(ALP)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的碱性磷酸酶(ALP)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的碱性磷酸酶(ALP)等多因子检测试剂盒(流式荧光发光法)呈正相关。
赠品
增值服务
相关产品
| 编号 | 适用物种：Bos taurus; Bovine (Cattle，牛) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB472Bo01 | 碱性磷酸酶(ALP)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB472Bo01 | 碱性磷酸酶(ALP)多克隆抗体 | WB; IHC; ICC; IP. |
| LAB472Bo71 | 碱性磷酸酶(ALP)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| SEB472Bo | 碱性磷酸酶(ALP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCB472Bo | 碱性磷酸酶(ALP)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMB472Bo | 碱性磷酸酶(ALP)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSB472Bo01 | 碱性磷酸酶(ALP)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Clinical Oral Implants Research | Comparison of platelet rich fibrin and collagen as osteoblast-seeded scaffolds for bone tissue engineering applications [Wiley: source] |
| Die Pharmazie - An International Journal of Pharmaceutical Sciences | The role of atorvastatin in bone metabolism in male albino Wistar rats [Ingenta: art00010] |
| 22 | Combined effect of strontium and zoledronate on hydroxyapatite structure and bone cell responses [ScienceDirect: S0142961214003135] |
| Biomed Mater.? | Directing chondrogenic differentiation of mesenchymal stem cells with a solid-supported chitosan thermogel for cartilage tissue engineering [Pubmed:24770944] |
| Journal of Colloid and Interface Science | Strontium and zoledronate hydroxyapatites graded composite coatings for bone prostheses [Pubmed:25706198] |
| Bone | The active role of osteoporosis in the interaction between osteoblasts and bone metastases [PubMed: 26057367] |
| Macromolecular Bioscience | Multi‐Layered Scaffolds for Osteochondral Tissue Engineering: In Vitro Response of Co‐Cultured Human Mesenchymal Stem Cells [PubMed: 26126665] |
| journal of hard Tissue Biology | Biological Evaluation of a Prototype Material made of Polyglycolic Acid and Hydroxyapatite [Article: Jhtb] |
| Colloids Surf B Biointerfaces | Antiresorption implant coatings based on calcium alendronate and octacalcium phosphate deposited by matrix assisted pulsed laser evaporation [PubMed: 26445021] |
| Gels | On the Mechanism of Drug Release from Polysaccharide Hydrogels Cross-Linked with Magnetite Nanoparticles by Applying Alternating Magnetic Fields: the Case of DOXO Delivery [2310-2861: 1] |
| Acta Biomater | Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: an in vitro osteoblast-osteoclast-endothelial cell co-culture study [PubMed: 26689470] |
| Sci Rep | A composite scaffold of MSC affinity peptide-modified demineralized bone matrix particles and chitosan hydrogel for cartilage regeneration [PubMed: 26632447] |
| Acta Biomaterialia | Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: An in vitro osteoblast–osteoclast–endothelial cell co-culture study [Pubmed:26689470] |
| PLoS One. | Extracorporeal shock waves alone or combined with raloxifene promote bone formation and suppress resorption in ovariectomized rats. [pubmed:28158228] |
| Scientific Reports | Adiponectin protects the rats liver against chronic intermittent hypoxia induced injury throughAMP-activated protein kinase pathway. [pubmed:27678302] |
| J Cell Physiol. | An advanced tri-culture model to evaluate the dynamic interplay among osteoblasts, osteoclasts, and endothelial cells. [pubmed:28240358] |
| Biomedicine & Pharmacotherapy | Anti-osteoporotic effects of an antidepressant tianeptine on ovariectomized rats [S0753332216322752] |
| Journal of Inorganic Biochemistry | Biomimetic fabrication of antibacterial calcium phosphates mediated by polydopamine. [pubmed:29049953] |
| Biomedicine & pharmacotherapy | Icaritin induces MC3T3-E1 subclone14 cell differentiation through estrogen receptor-mediated ERK1/2 and p38 signaling activation. [pubmed:28742995] |
| Journal of Clinical Periodontology | Peroxisome proliferator‐activated receptor γ plays dual roles on experimental periodontitis in rats [Pubmed:29574908] |
| Macromolecular Bioscience | Strontium‐Substituted Hydroxyapatite‐Gelatin Biomimetic Scaffolds Modulate Bone Cell Response [Pubmed:29877029] |
| Journal of Inorganic Biochemistry | Gradient coatings of strontium hydroxyapatite/zinc β-tricalcium phosphate as a tool to modulate osteoblast/osteoclast response [Pubmed:29525694] |
| DNA and Cell Biology | miRNA-133a-5p Inhibits the Expression of Osteoblast Differentiation-Associated Markers by Targeting the 3′ UTR of [Pubmed:29359964] |
| Bone | The sole and combined effect of simvastatin and platelet rich fibrin as a filling material in induced bone defect in tibia of albino rats [Pubmed: 30208342] |
| Experimental Gerontology | The decline of whole-body glucose metabolism in ovariectomized rats [Pubmed: 30292771] |
| BMC Complementary and Alternative Medicine | Hepatoprotective activity of Erythrina× neillii leaf extract and characterization of its phytoconstituents [Pubmed: 28095910] |
| Journal of Hematology & Oncology | Multiple myeloma-derived exosomes are enriched of amphiregulin (AREG) and activate the epidermal growth factor pathway in the bone microenvironment … [Pubmed: 30621731] |
| JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS | CoCr porous scaffolds manufactured via selective laser melting in orthopedics: Topographical, mechanical, and biological characterization [Pubmed: 30689288] |
| Chemico-Biological Interactions | Lead exposure inhibits osteoblastic differentiation and inactivates the canonical Wnt signal and recovery by icaritin in MC3T3-E1 subclone 14 cells [Pubmed: 30731080] |
| Journal of Orthopaedic Surgery and Research | Macrophage inhibits the osteogenesis of fibroblasts in ultrahigh molecular weight polyethylene (UHMWPE) wear particle-induced osteolysis [] |
| Bangladesh Journal of Medical Science | Zn as the Factor of Alkaline Phosphatase in Periodontal Patients consuming Minangkabau food in West Sumatra, Indonesia [] |
| Molecular Therapy-Nucleic Acids | AAV-anti-miR-214 prevents collapse of the femoral head in osteonecrosis by regulating osteoblast and osteoclast activities [Pubmed: 31739209] |
| Journal of Korean Neurosurgical Society | Rutin Improves Bone Histomorphometric Values by Reduction of Osteoclastic Activity in Osteoporosis Mouse Model Induced by Bilateral Ovariectomy [Pubmed: 32172552] |
| Journal of Applied Biomaterials & Functional Materials | Effects of tricalcium silicate/sodium alginate/calcium sulfate hemihydrate composite cements on osteogenic performances in vitro and in vivo [Pubmed: 32138579] |
| Nutrients | Osteoprotective Activity and Metabolite Fingerprint via UPLC/MS and GC/MS of Lepidium sativum in Ovariectomized Rats [Pubmed: 32668691] |
| International Journal of Clinical and Experimental Pathology | Matrilin-2 is a novel prognostic marker in osteosarcoma [] |
| Revista Argentina de Clínica Psicológica | Research on the Mechanism of miR-142-5p's Promotion of the Function and Mineralization of Osteoblasts by Down-regulating the Expression of WWP1 Gene [] |
| МАРКЕРЫ КОСТНОГО РЕМОДЕЛИРОВАНИЯ ПРИ ЗАМЕЩЕНИИ ДЕФЕКТАТРАБЕКУЛЯРНОЙ КОСТНОЙ ТКАНИ РЕЗОРБИРУЕМЫМИ И … [] | |
| Ceramics International | Platinum nanoparticles supported on functionalized hydroxyapatite: Anti-oxidant properties and bone cells response [] |
| Bioelectromagnetics | Static Magnetic Field (2¨C4 T) Improves Bone Microstructure and Mechanical Properties by Coordinating Osteoblast/Osteoclast Differentiation in Mice [33655538] |
| PeerJ | From hair to liver: emerging application of hair follicle mesenchymal stem cell transplantation reverses liver cirrhosis by blocking the TGF-β/Smad signaling … [Pubmed:35186473] |
| Cell Death Discov | ECM1 modified HF-MSCs targeting HSC attenuate liver cirrhosis by inhibiting the TGF-β/Smad signaling pathway [Pubmed:35136027] |
| Int J Mol Sci | Antiosteoporotic Nanohydroxyapatite Zoledronate Scaffold Seeded with Bone Marrow Mesenchymal Stromal Cells for Bone Regeneration: A 3D In Vitro Model [Pubmed:35682677] |
| Journal of Bionic Engineering | Effect of Antibacterial Enoxacin on the Properties of Injectable Nano-hydroxyapatite/Polyurethane Cement for Bone Repairing [] |
留言咨询