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血管紧张素转化酶(ACE)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Angiotensin I Converting Enzyme (ACE) ,etc. by FLIA (Flow Luminescence Immunoassay)

CD143; ACE1; DCP1; ACEI; ACE-I; Kininase II; Angiotensin-Converting Enzyme; Peptidyl-Dipeptidase A; Dipeptidyl Carboxypeptidase 1; Angiotensin-converting enzyme, soluble form

(注:单次混测多因子不超过8个指标 )

  • 血管紧张素转化酶(ACE)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • 血管紧张素转化酶(ACE)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测血管紧张素转化酶(ACE)等多因子检测试剂盒(流式荧光发光法),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的血管紧张素转化酶(ACE)等多因子检测试剂盒(流式荧光发光法)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 80-92 88
EDTA plasma(n=5) 89-98 95
heparin plasma(n=5) 86-101 96

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的血管紧张素转化酶(ACE)等多因子检测试剂盒(流式荧光发光法),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中血管紧张素转化酶(ACE)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 98-105% 84-98% 84-103% 82-98%
EDTA plasma(n=5) 83-90% 87-104% 86-97% 79-93%
heparin plasma(n=5) 88-97% 83-91% 83-97% 86-94%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备;
2. 加样(标准品、样本、磁珠)标准品或样本100μL及磁珠10μL,
    37°C酶标板振荡器孵育90分钟;
3. 磁吸甩干,加检测溶液A100μL,37°C酶标板振荡器孵育60分钟;
4. 磁吸洗板3次;
5. 加检测溶液B100μL,37°C振动孵育30分钟;
6. 磁吸洗板3次;
7. 加鞘液100μL,旋涡震荡2分钟后读数。

实验原理

将血管紧张素转化酶(ACE)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠,制成固相载体,向微孔中分别加入标准品或标本以及磁珠,其中的血管紧张素转化酶(ACE)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合,然后加入生物素化的血管紧张素转化酶(ACE)等多因子检测试剂盒(流式荧光发光法)抗体,将未结合的生物素化抗体洗净后,加入PE标记的亲和素,再次彻底洗涤后即可上机读数。MFI值和样品中的血管紧张素转化酶(ACE)等多因子检测试剂盒(流式荧光发光法)呈正相关。

赠品

相关产品

编号 适用物种:Bos taurus; Bovine (Cattle,牛) 应用(仅供研究使用,不用于临床诊断!)
RPA004Bo01 血管紧张素转化酶(ACE)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA004Bo01 血管紧张素转化酶(ACE)多克隆抗体 WB; IHC; ICC; IP.
MAA004Bo21 血管紧张素转化酶(ACE)单克隆抗体 WB; IHC; ICC; IP.
LMA004Bo 血管紧张素转化酶(ACE)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Lipids in Health and disease Association of elevated blood pressure and impaired vasorelaxation in experimental Sprague-Dawley rats fed with heated vegetable oil [PubMed: PMC2914008]
Malaysian Journal of Medical Sciences Effect of Repeatedly Heated Palm Olein on Blood Pressure–Regulating Enzymes Activity and Lipid Peroxidation in Rats [Usm: MJMS19120]
Journal of Cancer Therapy Plasma Levels of Angiotensin-Converting Enzymes 1 and 2 and AGTR2 (T1247G and A5235G) Gene Polymorphisms Are Associated to Breast Cancer Progression. [Scirp: Source]
The American journal of clinical nutrition Vascular function and atherosclerosis progression after 1 y of flavonoid intake in statin-treated postmenopausal women with type 2 diabetes: a double-blind randomized controlled trial. [Pubmed: 23553151]
Journal of Vascular Surgery Cathepsin G deficiency reduces periaortic calcium chloride injury-induced abdominal aortic aneurysms in mice [Pubmed:25037606]
Reprod Toxicol.? ACE2 activation by xanthenone prevents leptin-induced increases in blood pressure and proteinuria during pregnancy in Sprague-Dawley rats [Pubmed:25205467]
Chemical Research in Toxicology Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal Instillation [Pubmed:25580880]
Chem Biol Interact Arsenic causes aortic dysfunction and systemic hypertension in rats: Augmentation of angiotensin II signaling [PubMed: 26079204]
Biomed Res Int Protective Effect of Enalapril against Methionine-Enriched Diet-Induced Hypertension: Role of Endoplasmic Reticulum and Oxidative Stress [PubMed: 26640794]
Clinics (Sao Paulo) Mechanisms [PubMed: 26602523]
Chemical Research in Toxicology Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal Instillation [PubMed: 25580880]
Intensive Care Medicine Experimental Angiotensin converting enzymes in patients with acute respiratory distress syndrome [Content: 3]
Evid Based Complement Alternat Med. Garlic Attenuates Plasma and Kidney ACE-1 and AngII Modulations in Early Streptozotocin-Induced Diabetic Rats: Renal Clearance and Blood Pressure Implications [Pubmed:27293465]
Вавиловский журнал генетики и селекции Ренин-ангиотензин-альдостероновая система у крыс линии НИСАГ (ISIAH) со стресс-индуцированной артериальной гипертензией [30_Dubinina.pdf]
Animal Biology The effect of heat stress on bull sperm quality and related HSPs expression [content:journals]
biomedicine & pharmacotherapy Citrus leaf extract reduces blood pressure and vascular damage in repeatedly heated palm oil diet-Induced hypertensive rats. [pubmed:28068636]
Biomedicine & Pharmacotherapy Parkia speciosa empty pod prevents hypertension and cardiac damage in rats given N(G)-nitro- L -arginine methyl ester [pubmed:28992471]
Journal of Functional Foods Antihypertensive effects of Ocimum gratissimumextract: Angiotensin-converting enzyme inhibitor in vitro and in vivo investigation [10.1016/j.jff.2017.05.033]
Molecules Protective Effect of Polyphenol-Rich Extract from Bee Pollen in a High-Fat Diet [Pubmed:29614743]
Journal of Surgical Research Impact of angiotensin-converting enzyme 2 levels on postoperative pneumonia after esophagectomy [Pubmed:29506841]
Intensive Care Medicine Angiotensin-converting enzymes in acute respiratory distress syndrome []
International Journal of Biological Sciences Vascular Protection of TPE-CA on Hyperhomocysteinemia-induced Vascular Endothelial Dysfunction Through AA Metabolism Modulated CYPs Pathway []
Medicine Alteration and association between serum ACE2/angiotensin (1-7)/Mas axis and oxidative stress in chronic kidney disease: A pilot study [Pubmed: 32756181]
American Journal of Respiratory and Critical Care Medicine Equilibrium Angiotensin Metabolite Profiling in Patients with Acute Respiratory Distress Syndrome Indicates Angiotensin Converting Enzyme Inhibition [Pubmed: 32628511]
Int Immunopharmacol Crocin protects against cardiotoxicity induced by doxorubicin through TLR-2/NF-κB signal pathway in vivo and vitro [Pubmed: 32388215]
Food Bioscience Djulis (Chenopodium formosanum) and its bioactive compounds affect vasodilation, angiotensin converting enzyme activity, and hypertension []
Journal of Extracellular Vesicles MiR155-5p in adventitial fibroblasts-derived extracellular vesicles inhibits vascular smooth muscle cell proliferation via suppressing angiotensin-converting enzyme … []
Biotechnology and Pharmacy Chrysin pretreatment improves mitochondrial enzymes and angiotensin converting enzymes in L-NAME induced hypertensive rats []
Dikkat Eksikliği Hiperaktivite Bozukluğu Tanısı Olan Anjiyotensin I, II, III, Alamandin peptid, Anjiyotensin Dönüştürücü Enzim (ACE), NADPH Oksidaz Enzim Serum … []
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