不对称二甲基精氨酸(ADMA)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Asymmetrical Dimethylarginine (ADMA) ,etc. by FLIA (Flow Luminescence Immunoassay)
N,N-Dimethylarginine; NG,NG-Dimethylarginine dihydrochloride; Asymmetric Dimethylarginine
（注：单次混测多因子不超过8个指标 ）
- 编号LMB301Ge
- 物种Pan-species (General，通用) 相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长1.5h
- 检测范围0.98-1000ng/mL
- 灵敏度最小可检测剂量小于等于0.327 ng/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
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产品包装(模拟)
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产品包装(模拟)
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测不对称二甲基精氨酸(ADMA)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的不对称二甲基精氨酸(ADMA)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 87-95 | 90 |
| EDTA plasma(n=5) | 79-102 | 82 |
| heparin plasma(n=5) | 83-92 | 87 |
| sodium citrate plasma(n=5) | 83-90 | 86 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的不对称二甲基精氨酸(ADMA)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中不对称二甲基精氨酸(ADMA)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 94-104% | 87-101% | 84-101% | 93-101% |
| EDTA plasma(n=5) | 82-90% | 95-102% | 93-101% | 79-103% |
| heparin plasma(n=5) | 81-90% | 81-98% | 89-102% | 78-93% |
| sodium citrate plasma(n=5) | 89-97% | 78-98% | 96-104% | 93-101% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠、检测溶液A）标准品或样本50μL及磁珠10μL，加检测溶液A50μL，
37°C酶标板振荡器孵育60分钟；
3. 磁吸洗板3次；
4. 加检测溶液B100μL，37°C振动孵育30分钟；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋涡震荡2分钟后读数。
实验原理
将不对称二甲基精氨酸(ADMA)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠、标记VEGFA，标记的不对称二甲基精氨酸(ADMA)等多因子检测试剂盒(流式荧光发光法)和未标记的不对称二甲基精氨酸(ADMA)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体竞争结合，然后将未结合物洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的不对称二甲基精氨酸(ADMA)等多因子检测试剂盒(流式荧光发光法)呈负相关。
赠品
增值服务
相关产品
| 编号 | 适用物种：Pan-species (General，通用) | 应用(仅供研究使用，不用于临床诊断！) |
| CPB301Ge11 | 不对称二甲基精氨酸(ADMA)牛血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| CPB301Ge21 | 不对称二甲基精氨酸(ADMA)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| PAB301Ge01 | 不对称二甲基精氨酸(ADMA)多克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| LAB301Ge71 | 不对称二甲基精氨酸(ADMA)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| CEB301Ge | 不对称二甲基精氨酸(ADMA)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB301Ge | 不对称二甲基精氨酸(ADMA)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSB301Ge11 | 不对称二甲基精氨酸(ADMA)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
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| Neurol Res. | Effect of 18β-glycyrrhetinic acid on cerebral vasospasm caused by asymmetric dimethylarginine after experimental subarachnoid hemorrhage in rats. [Pubmed:25475507] |
| Int J Clin Exp Med. | Correlation research on ADMA plasma levels and left ventricular function of peritoneal dialysis patients [Pubmed:25550968] |
| Clin Respir J | Asymmetric dimethylarginine contributes to airway nitric oxide deficiency in patients with COPD [PubMed: 26076870] |
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| Neurological Research | Effect of 18β-glycyrrhetinic acid on cerebral vasospasm caused by asymmetric dimethylarginine after experimental subarachnoid hemorrhage in rats [PubMed: 25475507] |
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| Biochemical and Biophysical Research Communications | Probucol improves erectile function via Activation of Nrf2 and coordinates the HO-1/DDAH/PPAR-γ/eNOS pathways in streptozotocin-induced diabetic rats [Pubmed: 30454888] |
| Brazilian Journal of Pharmaceutical Science | Demographic, clinical and lifestyle predictors for severity of erectile dysfunction and biomarkers level in Malaysian patients [] |
| ACS Combinatorial Science | Novel cellularly active inhibitor regresses DDAH1 induced prostate tumor growth by restraining tumor angiogenesis through targeting DDAH1/ADMA/NOS pathway [Pubmed: 30673277] |
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| Oxid Med Cell Longev | The Role of Reactive Oxygen Species and Nitric Oxide in the Inhibition of Trichophyton rubrum Growth by HaCaT Cells [Pubmed: 32104540] |
| Molecular Medicine Reports | Blueberry anthocyanin‑enriched extract ameliorates transverse aortic constriction‑induced myocardial dysfunction via the DDAH1/ADMA/NO signaling pathway in … [Pubmed: 31746378] |
| International Journal of Nephrology | Effect of a Supervised Peridialytic Exercise Program on Serum Asymmetric Dimethylarginine in Maintenance Hemodialysis Patients [Pubmed: 33163233] |
| Journal of Clinical Medicine | The Usefulness of Serum Biomarkers in the Early Stages of Diabetic Retinopathy: Results of the EUROCONDOR Clinical Trial [Pubmed: 32344735] |
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