癌胚抗原(CEA)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Carcinoembryonic Antigen (CEA) ,etc. by FLIA (Flow Luminescence Immunoassay)
CD66E; CD66; CEACAM5; Carcinoembryonic Antigen-related Cell Adhesion Molecule 5; Carcinoembryonic Antigen
（注：单次混测多因子不超过8个指标 ）
- 编号LMA150Hu
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3.5h
- 检测范围1.22-1250pg/mL
- 灵敏度最小可检测剂量小于等于0.407 pg/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
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产品包装(模拟)
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产品包装(模拟)
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测癌胚抗原(CEA)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的癌胚抗原(CEA)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 82-103 | 94 |
| EDTA plasma(n=5) | 78-97 | 86 |
| heparin plasma(n=5) | 95-102 | 99 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的癌胚抗原(CEA)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中癌胚抗原(CEA)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 86-104% | 89-97% | 78-96% | 79-98% |
| EDTA plasma(n=5) | 79-97% | 86-101% | 78-89% | 93-103% |
| heparin plasma(n=5) | 92-102% | 80-99% | 85-92% | 98-105% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。
实验原理
将癌胚抗原(CEA)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的癌胚抗原(CEA)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的癌胚抗原(CEA)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的癌胚抗原(CEA)等多因子检测试剂盒(流式荧光发光法)呈正相关。
赠品
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| APA150Hu61 | 癌胚抗原(CEA)活性蛋白 | Cell culture; Activity Assays. |
| EPA150Hu61 | 癌胚抗原(CEA)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA150Hu01 | 癌胚抗原(CEA)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA150Hu04 | 癌胚抗原(CEA)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA150Hu03 | 癌胚抗原(CEA)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA150Hu02 | 癌胚抗原(CEA)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA150Hu02 | 癌胚抗原(CEA)活性蛋白 | Cell culture; Activity Assays. |
| APA150Hu04 | 癌胚抗原(CEA)活性蛋白 | Cell culture; Activity Assays. |
| RPA150Hu05 | 癌胚抗原(CEA)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA150Hu04 | 癌胚抗原(CEA)多克隆抗体 | WB |
| PAA150Hu03 | 癌胚抗原(CEA)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA150Hu01 | 癌胚抗原(CEA)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA150Hu02 | 癌胚抗原(CEA)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA150Hu71 | 癌胚抗原(CEA)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| RAA150Hu21 | 癌胚抗原(CEA)重组抗体 | WB; IF; ICC; IHC; IP; FCM. |
| MAA150Hu22 | 癌胚抗原(CEA)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA150Hu | 癌胚抗原(CEA)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA150Hu | 癌胚抗原(CEA)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA150Hu01 | 癌胚抗原(CEA)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| BioFactors | Ginger ingredients inhibit the development of diethylnitrosoamine induced premalignant phenotype in rat chemical hepatocarcinogenesis model [PubMed: 20872761] |
| Life Sciences | Comparison of angiotensin converting enzyme inhibitors and angiotensin II type 1 receptor blockade for the prevention of premalignant changes in the liver [ScienceDirect: S0024320511002700] |
| Iranian journal of basic medical sciences | Saffron Aqueous Extract Inhibits the Chemically-induced Gastric Cancer Progression in the Wistar Albino Rat [PubMed: PMC3637902] |
| Biosensors and Bioelectronics | Handy, rapid and multiplex detection of tumor markers based on encoded silica-hydrogel hybrid beads array chip [Pubmed: 23672876] |
| Asian Pac J Cancer Prev. | Association of Paraoxonase-1 (Q192R and L55M) Gene Polymorphisms and Activity with Colorectal Cancer and Effect of Surgical Intervention. [Pubmed:25684529] |
| Journal of Analytical Atomic Spectrometry | Detection of HIV-1 p24 antigen using streptavidin–biotin and gold nanoparticles based immunoassay by inductively coupled plasma mass spectrometry [Rsc:Source] |
| Breast | Activated Leukocyte Cell Adhesion Molecule (ALCAM) in Saudi Breast Cancer Patients as Prognostic and Predictive Indicator [PubMed: 26446295] |
| The Journal of Toxicological Sciences | Gene expression analysis in the lung of the rasH2 transgenic mouse at week 4 prior to induction of malignant tumor formation by urethane and N-methylolacrylamide [PubMed: 26558449] |
| PLoS One | Collecting Protein Biomarkers in Breath Using Electret Filters: A Preliminary Method on New Technical Model and Human Study [Pubmed:26934615] |
| Journal of Applied Pharmaceutical Science | Chemopreventive effect of Indigofera linnaei extract against diethylnitrosamine induced hepatocarcinogenesis in rats [2071_pdf.pdf] |
| Analytical chemistry | Temporal Sensing Platform Based on Bipolar Electrode for the Ultrasensitive Detection of Cancer Cells [pubmed:27506255] |
| Frontiers in Physiology | Anticarcinogenic potential of ethanol extract of Indigofera cordifolia Roth.(Fabales: Fabaceae) on diethylnitrosamine induced hepatocarcinogenesis in rats [v04n07a07a] |
| Analytica Chimica Acta | Ultrasensitive cathode photoelectrochemical immunoassay based on TiO2 photoanode-enhanced 3D Cu2O nanowire array photocathode and signal amplification by … [Pubmed:29866267] |
| Journal of Cancer | Evaluation of Detection Methods and Values of Circulating Vascular Endothelial Growth Factor in Lung Cancer [Pubmed:29675110] |
| Clinical Breast Cancer | 8-Hydroxy-2'-deoxyguanosine as a Discriminatory Biomarker For Early Detection of Breast Cancer [] |
| Journal of Ethnopharmacology | Preventive potential of dietary inclusion of Brachystegia eurycoma (Harms) seeds on N-methyl-N-nitrosourea-induced colon carcinogenesis in Wistar rats [Pubmed: 30953819] |
| Asian Journal of Pharmacy and Pharmacology | Chemopreventive effects of on diethylnitrosamine induced and Indigofera cassioides phenobarbital promoted rat liver carcinoma [] |
| Biomaterials | DNA-templated porous nanoplatform towards programmed “double-hit” cancer therapy via hyperthermia and immunogenicity activation [Pubmed: 31374481] |
| Biology | Piperine Regulates Nrf-2/Keap-1 Signalling and Exhibits Anticancer Effect in Experimental Colon Carcinogenesis in Wistar Rats [Pubmed: 32967203] |
| Oncology Research and Treatment | Evaluation of the Diagnostic and Predicative Values of 8-Iso-Prostaglandin F2α as a Biomarker of Breast Cancer [Pubmed: 32721979] |
| Journal of Functional Foods | Chemopreventive effect of dietary inclusion with Crassocephalum rubens (Juss ex Jacq) leaf on N-methyl-N-nitrosourea (MNU)-induced colorectal carcinogenesis in … [] |
| Lateral Flow Immunosensor for Ferritin Based on Dual Signal-Amplified Strategy by Rhodium Nanoparticles [] | |
| Anti-Cancer Activity of Piperine Against Colon Carcinogenesis Via Modulation of NF-κB/Nrf-2/Keap1/HO-1 Signalling Pathways [] | |
| Microchemical Journal | Magnetic immunoassay for tumor clinical diagnosis based on rolling circular amplification (RCA) coupled with ICP-MS [] |
| Anticancer efficacy of β-Sitosterol Loaded Hydroxyapatite-Alginate on Colon Cancer Cell in Vivo [] | |
| sensors and actuators b-chemical | A light-induced self-powered competitive immunosensor for the detection of platelet derived growth factor-BB via an elaborately assembled bioconjugate [] |