太阳集团tyc5997_平台(china)官方网站

D-二聚体(D2D)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for D-Dimer (D2D) ,etc. by FLIA (Flow Luminescence Immunoassay)

D 2 Dimer

（注：单次混测多因子不超过8个指标 ）

  • D-二聚体(D2D)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • D-二聚体(D2D)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测D-二聚体(D2D)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的D-二聚体(D2D)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 91-99 94
EDTA plasma(n=5) 96-103 99
heparin plasma(n=5) 93-104 101
sodium citrate plasma(n=5) 89-101 92

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的D-二聚体(D2D)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中D-二聚体(D2D)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 86-101% 86-93% 80-91% 98-105%
EDTA plasma(n=5) 91-99% 79-95% 78-92% 96-103%
heparin plasma(n=5) 94-101% 98-105% 98-105% 90-103%
sodium citrate plasma(n=5) 78-91% 84-103% 78-101% 87-101%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠、检测溶液A）标准品或样本50μL及磁珠10μL，加检测溶液A50μL，
    37°C酶标板振荡器孵育60分钟；
3. 磁吸洗板3次；
4. 加检测溶液B100μL，37°C振动孵育30分钟；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋涡震荡2分钟后读数。

实验原理

将D-二聚体(D2D)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠、标记VEGFA，标记的D-二聚体(D2D)等多因子检测试剂盒(流式荧光发光法)和未标记的D-二聚体(D2D)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体竞争结合，然后将未结合物洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的D-二聚体(D2D)等多因子检测试剂盒(流式荧光发光法)呈负相关。

赠品

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
CPA506Hu21 D-二聚体(D2D)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA506Hu01 D-二聚体(D2D)多克隆抗体 WB; IHC; ICC; IP.
LAA506Hu71 D-二聚体(D2D)多克隆抗体(生物素标记) WB; IHC; ICC.
CEA506Hu D-二聚体(D2D)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA506Hu D-二聚体(D2D)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA506Hu11 D-二聚体(D2D)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
22 Determinants of the thrombogenic potential of multiwalled carbon nanotubes [ScienceDirect: S0142961211004819]
Journal of Thrombosis and Haemostasis Exogenous Activated Protein C Inhibits the Progression of Diabetic Nephropathy [Pubmed: 22236035]
PLOS ONE Protective Effect of Curcumin on Pulmonary and Cardiovascular Effects Induced by Repeated Exposure to Diesel Exhaust Particles in Mice [PlosOne: Source]
Haemetology Clinical impact of factor V Leiden, prothrombin G20210A, and MTHFR C677T mutations among sickle cell disease patients of Central India [Pubmed: 23992124]
Journal of Trauma and Acute Care Surgery Assessment of coagulopathy, endothelial injury, and inflammation after traumatic brain injury and hemorrhage in a porcine model [Lww:Source]
Journal of Thrombosis and Haemostasis Protein S exacerbates alcoholic hepatitis by stimulating liver natural killer T cells [Pubmed:25399514]
Inflammation.? Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis [Pubmed:25448261]
Medical Science Monitor Impact of β-Adrenoceptor Blockade on Systemic Inflammation and Coagulation Disturbances in Rats with Acute Traumatic Coagulopathy [Pubmed:25676919]
Blood Intraperitoneal administration of activated protein C prevents postsurgical adhesion band formation. [PubMed: 25575539]
Am J Physiol Regul Integr Comp Physiol Procoagulant and Fibrinolytic Activity after Polytrauma in Rat [PubMed: 26632604]
hypertension Research Upregulation of canonical transient receptor potential channel in the pulmonary arterial smooth muscle of a chronic thromboembolic pulmonary hypertension rat model [PubMed: 26155749]
Molecular Nutrition & Food Research Trans‐fatty acid promotes thrombus formation in mice by aggravating antithrombogenic endothelial functions via Toll‐like receptors [PubMed: 25546502]
JCI Insight. KLF2 and KLF4 control endothelial identity and vascular integrity. [pubmed:28239661]
The American Society of Anesthesiologists Activated protein C drives the hyperfibrinolysis of acute traumatic coagulopathy [pubmed:27841821]
32 Activated TAFI Promotes the Development of Chronic Thromboembolic Pulmonary Hypertension: A Possible Novel Therapeutic Target [pubmed:28289017]
The American Journal of Pathology Inhibition of Cell Apoptosis and Amelioration of Pulmonary Fibrosis by Thrombomodulin [pubmed:28739343]
Atherosclerosis Low dose of alcohol attenuates pro-atherosclerotic activity of thrombin. [pubmed:28923781]
28 Lactadherin Promotes Microvesicle Clearance to Prevent Coagulopathy and Improves Survival of Severe TBI Mice [pubmed:29162596]
Journal of Thrombosis and Thrombolysis Guided longer pulses from a diagnostic ultrasound and intraclot microbubble enhanced catheter-directed thrombolysis in vivo. [pubmed:28417266]
Journal of Investigative Surgery Experimental Evaluation of a New Tissue Factor-Based Topical Hemostat (TT-173) for Treatment of Hepatic Bleeding [Pubmed: 30380352]
Marine Drugs The Inhibitory Effect of Protamine on Platelets is Attenuated by Heparin without Inducing Thrombocytopenia in Rodents [Pubmed: 31533230]
Intensive Care Med Exp Complement factor D is linked to platelet activation in human and rodent sepsis [34396466]
Thromb Res NLRP3 Inflammasome contributes to endotoxin-induced coagulation [Pubmed:35421682]
Experimental and Therapeutic Medicine Construction of a rabbit model with vinorelbine administration via peripherally inserted central catheter and dynamic monitoring of changes in phlebitis and thrombosis [Pubmed:35126715]
留言咨询
Baidu
sogou