红细胞生成素(EPO)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Erythropoietin (EPO) ,etc. by FLIA (Flow Luminescence Immunoassay)
EP; Epoetin; Erythropoetin; Hematopoietin; Hemopoietin
（注：单次混测多因子不超过8个指标 ）
- 编号LMA028Hu
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3.5h
- 检测范围1.95-2000pg/mL
- 灵敏度最小可检测剂量小于等于0.65 pg/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
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产品包装(模拟)
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产品包装(模拟)
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测红细胞生成素(EPO)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的红细胞生成素(EPO)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 85-101 | 95 |
| EDTA plasma(n=5) | 92-101 | 96 |
| heparin plasma(n=5) | 81-96 | 85 |
| sodium citrate plasma(n=5) | 93-101 | 97 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的红细胞生成素(EPO)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中红细胞生成素(EPO)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 93-101% | 81-90% | 79-90% | 87-95% |
| EDTA plasma(n=5) | 92-101% | 93-101% | 97-105% | 90-99% |
| heparin plasma(n=5) | 93-104% | 91-101% | 88-103% | 78-105% |
| sodium citrate plasma(n=5) | 96-103% | 85-98% | 83-93% | 81-102% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。
实验原理
将红细胞生成素(EPO)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的红细胞生成素(EPO)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的红细胞生成素(EPO)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的红细胞生成素(EPO)等多因子检测试剂盒(流式荧光发光法)呈正相关。
赠品
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| APA028Hu61 | 红细胞生成素(EPO)活性蛋白 | Cell culture; Activity Assays. |
| EPA028Hu61 | 红细胞生成素(EPO)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA028Hu01 | 红细胞生成素(EPO)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA028Hu01 | 红细胞生成素(EPO)活性蛋白 | Cell culture; Activity Assays. |
| APA028Hu62 | 红细胞生成素(EPO)活性蛋白 | Cell culture; Activity Assays. |
| PAA028Hu06 | 红细胞生成素(EPO)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA028Hu01 | 红细胞生成素(EPO)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA028Hu71 | 红细胞生成素(EPO)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAA028Hu23 | 红细胞生成素(EPO)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA028Hu26 | 红细胞生成素(EPO)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA028Hu21 | 红细胞生成素(EPO)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA028Hu24 | 红细胞生成素(EPO)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA028Hu25 | 红细胞生成素(EPO)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA028Hu22 | 红细胞生成素(EPO)单克隆抗体 | WB,IHC |
| SEA028Hu | 红细胞生成素(EPO)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA028Hu | 红细胞生成素(EPO)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA028Hu | 红细胞生成素(EPO)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA028Hu01 | 红细胞生成素(EPO)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Endocrinology | Prenatal Glucocorticoid Overexposure Causes Permanent Increases in Renal Erythropoietin Expression and Red Blood Cell Mass in the Rat Offspring [Endo: source] |
| Journal of Ethnopharmacology | Inhibitory effect of polysaccharides isolated from Angelica sinensis on hepcidin expression [PubMed: 21333724] |
| PLOS ONE | CD98 Positive Eosinophils Contribute to T Helper 1 Pattern Inflammation [PlosOne: Source] |
| The Journal of Clinical Endocrinology & Metabolism | Alterations of Circulating Endothelial Cell and Endothelial Progenitor Cell Counts around the Ovulation [PubMed: 22948762] |
| Experimental & Molecular Medicine | Long-term and stable correction of uremic anemia by intramuscular injection of plasmids containing hypoxia-regulated system of erythropoietin expression [PubMed: PMC3509184] |
| Biochem Biophys Res Commun. | Reevaluation of erythropoietin production by the nephron [Pubmed:24832733] |
| Nutrition. | Carbohydrate and glutamine supplementation modulates the Th1/Th2 balance after exercise performed at a simulated altitude of 4500 m. [Pubmed:2528040] |
| J Appl Physiol (1985). | Decreased plasma soluble erythropoietin receptor in high-altitude excessive erythrocytosis and Chronic Mountain Sickness [Pubmed:25324511] |
| Expert Rev Proteomics | Plasma hepcidin in early-stage breast cancer patients: no relationship with interleukin-6, erythropoietin and erythroferrone [PubMed: 26496240] |
| Oncotarget | KIAA0101 is associated with human renal cell carcinoma proliferation and migration induced by erythropoietin [pubmed:26575329] |
| international journal of molecular sciences | Expression of Iron-Related Proteins Differentiate Non-Cancerous and Cancerous Breast Tumors. [pubmed:28216608] |
| Veterinary Clinical Pathology | Occult gastrointestinal bleeding is a common finding in dogs with chronic kidney disease [pubmed:28186645] |
| IOS Press Content Library | An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … [articles:journal-of-alzheimers-disease] |
| Oxidative Medicine and Cellular Longevity | Acidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-κB Activation [pubmed:29147463] |
| PLoS Neglected Tropical Diseases | Functional and phenotypic evaluation of eosinophils from patients with the acute form of paracoccidioidomycosis [pubmed:28489854] |
| Neurochemistry International | Effects of erythropoietin on astrocytes and brain endothelial cells in primary culture during anoxia depend on simultaneous signaling by other cytokines and on duration of anoxia [S0197018617304783] |
| Pesquisa Veterinária Brasileira | Relation between anaemia and bone marrow features and serum erythropoietin in dogs with chronic kidney disease [10.1590/s0100-736x2017000600011 ] |
| Medical Journal Armed Forces India | Study of acute hypoxia markers in healthy subjects: Utility in post-crash investigation [10.1016/j.mjafi.2017.04.003] |
| Neurochemistry International | Effects of erythropoietin on astrocytes and brain endothelial cells in primary culture during anoxia depend on simultaneous signaling by other cytokines and on … [Pubmed:29180303] |
| Clinical and Experimental Medicine | Increased NGAL level associated with iron store in chronic kidney disease with anemia [Pubmed:29909502] |
| Bulletin of Experimental Biology and Medicine | Signal Mechanism of the Protective Effect of Combined Preconditioning by Amtizole and Moderate Hypoxia [Pubmed:29308565] |
| Bulletin of Experimental Biology and Medicine | Expression of Hif-1α, Nf-κb, and Vegf Genes in the Liver and Blood Serum Levels of HIF-1α, Erythropoietin, VEGF, TGF-β, 8-Isoprostane, and Corticosterone in Wistar Rats with High and Low Resistance to Hypoxia. Corticosterone in … [Pubmed: 30353332] |
| FASEB JOURNAL | High-altitude hypoxia decreases plasma erythropoietin soluble receptor (sEpoR) concentration in humans [] |
| Journal of Functional Foods | Dietary gelatin enhances non-heme iron absorption possibly via regulation of systemic iron homeostasis in rats [] |
| 博士论文 | PHYSIOLOGICAL ADAPTATIONS TO HEAT ACCLIMATION; REPERCUSSIONS ON CYCLING PERFORMANCE [] |
| Nutrition | Effects of carbohydrate and glutamine supplementation on cytokine production by monocytes after exercise in hypoxia: a crossover, randomized and double-blind … [Pubmed: 31743809] |
| BRITISH JOURNAL OF HAEMATOLOGY | Disordered serum erythroferrone and hepcidin levels as indicators of the spontaneous abortion occurrence during early pregnancy in humans [Pubmed: 32866306] |
| JCI Insight. | Increased FGF-23 levels are linked to ineffective erythropoiesis and impaired bone mineralization in myelodysplastic syndromes [Pubmed: 32759495] |
| Blood Cells, Molecules, and Diseases | EPAS1 regulates proliferation of erythroblasts in chronic mountain sickness [Pubmed: 32470757] |
| Int J Endocrinol | miR663 Prevents Epo Inhibition Caused by TNF-Alpha in Normoxia and Hypoxia [34367277] |
| J Mater Sci Mater Med | Loading of erythropoietin on biphasic calcium phosphate bioceramics promotes osteogenesis and angiogenesis by regulating EphB4/EphrinB2 molecules [Pubmed:35072831] |
| Effect of arsenate on erythropoietin production and autophagy induction in HepG2 cells [] |
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