γ-氨基丁酸(gABA)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Gamma-Aminobutyric Acid (gABA) ,etc. by FLIA (Flow Luminescence Immunoassay)
4-Aminobutyric Acid
（注：单次混测多因子不超过8个指标 ）
- 编号LMA900Ge
- 物种Pan-species (General，通用) 相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长1.5h
- 检测范围1.95-2000pg/mL
- 灵敏度最小可检测剂量小于等于0.65 pg/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
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产品包装(模拟)
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产品包装(模拟)
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测γ-氨基丁酸(gABA)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的γ-氨基丁酸(gABA)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 96-105 | 101 |
| EDTA plasma(n=5) | 82-101 | 97 |
| heparin plasma(n=5) | 86-101 | 93 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的γ-氨基丁酸(gABA)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中γ-氨基丁酸(gABA)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 96-104% | 95-105% | 86-96% | 89-98% |
| EDTA plasma(n=5) | 96-105% | 91-98% | 80-98% | 83-98% |
| heparin plasma(n=5) | 94-105% | 78-101% | 80-91% | 87-95% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠、检测溶液A）标准品或样本50μL及磁珠10μL，加检测溶液A50μL，
37°C酶标板振荡器孵育60分钟；
3. 磁吸洗板3次；
4. 加检测溶液B100μL，37°C振动孵育30分钟；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋涡震荡2分钟后读数。
实验原理
将γ-氨基丁酸(gABA)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠、标记VEGFA，标记的γ-氨基丁酸(gABA)等多因子检测试剂盒(流式荧光发光法)和未标记的γ-氨基丁酸(gABA)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体竞争结合，然后将未结合物洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的γ-氨基丁酸(gABA)等多因子检测试剂盒(流式荧光发光法)呈负相关。
赠品
增值服务
相关产品
| 编号 | 适用物种：Pan-species (General，通用) | 应用(仅供研究使用，不用于临床诊断！) |
| CPA900Ge11 | γ-氨基丁酸(gABA)牛血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| CPA900Ge21 | γ-氨基丁酸(gABA)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| CPA900Ge41 | γ-氨基丁酸(gABA)人血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| PAA900Ge01 | γ-氨基丁酸(gABA)多克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| PAA900Ge02 | γ-氨基丁酸(gABA)多克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| LAA900Ge71 | γ-氨基丁酸(gABA)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAA900Ge81 | γ-氨基丁酸(gABA)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| CEA900Ge | γ-氨基丁酸(gABA)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| AEA900Ge | 抗γ-氨基丁酸抗体(Anti-gABA)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| LMA900Ge | γ-氨基丁酸(gABA)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA900Ge11 | γ-氨基丁酸(gABA)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Toxicology and Industrial Health | Effect of sub-acute exposure to acrylamide on GABAergic neurons and astrocytes in weaning rat cerebellum [PubMed: 21444355] |
| Neurosci Lett | Expression of the neuron-specific potassium chloride cotransporter KCC2 in adult rat cochlear. [PubMed: 18577424] |
| Biomark Insights | Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with Autism [PubMed: PMC3623607] |
| Biomarker insights | Correlation Between Hepatocyte Growth Factor (HGF) and Gamma-Aminobutyric Acid (GABA) Plasma Levels in Autistic Children [PubMed: PMC3694825] |
| Biomarker insights | Decreased Hepatocyte Growth Factor (HGF) and Gamma Aminobutyric Acid (GABA) in Individuals with Obsessive-Compulsive Disorder (OCD) [PubMed: PMC3762604] |
| Scientific Reports | Renal Denervation Improves the Baroreflex and GABA System in Chronic Kidney Disease-induced Hypertension [pubmed:27917928] |
| Journal of Neuroscience | Sex differences in the glutamate signaling pathway in juvenile rats [Pubmed:28861894] |
| Scientific Reports | A novel homozygous mutation in GAD1 gene described in a schizophrenic patient impairs activity and dimerization of GAD67 enzyme [Pubmed: 30341396] |
| Neurochemical Research | Aucubin Alleviates Seizures Activity in Li-Pilocarpine-Induced Epileptic Mice: Involvement of Inhibition of Neuroinflammation and Regulation of Neurotransmission [Pubmed: 30666488] |
| Neuroscience Letters | Differentiation of human glioblastoma U87 cells into cholinergic neuron [Pubmed: 30928478] |
| Neurochemical Journal | Glutamate Signaling Defects in Propionic Acid Orally Administered to Juvenile Rats as an Experimental Animal Model of Autism [] |
| Effect of gamma radiation on combination therapy of certain antiepileptic drugs in rats. [] | |
| Experimental and Therapeutic Medicine | Antiepileptic effects of exogenous β‑hydroxybutyrate on kainic acid‑induced epilepsy [Pubmed: 33101467] |
| Exp Anim | Dementia model mice exhibited improvements of neuropsychiatric symptoms as well as cognitive dysfunction with neural cell transplantation [33828024] |
| Neurogenetics | Age-dependent neurological phenotypes in a mouse model of PRRT2-related diseases [34101060] |
| Sci Rep | Overexpression of wild-type human amyloid precursor protein alters GABAergic transmission [34475508] |
| J Food Biochem | Adaptogenic potential of ginsenosides against domoic acid‐induced toxicity by regulating neuronal stress and kinate receptors: Ex vivo and in silico studies [Pubmed:35102569] |