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促性腺激素释放激素(GnRH)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Gonadotropin Releasing Hormone (GnRH) ,etc. by FLIA (Flow Luminescence Immunoassay)

GNRH1; GRH; LNRH; LHRH; LH-RH I; Luliberin; Luteinizing-Hormone Releasing Hormone; Progonadoliberin-1; Gonadoliberin I; Gonadorelin; GnRH-associated peptide 1

（注：单次混测多因子不超过8个指标 ）

  • 促性腺激素释放激素(GnRH)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • 促性腺激素释放激素(GnRH)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测促性腺激素释放激素(GnRH)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的促性腺激素释放激素(GnRH)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 96-103 101
EDTA plasma(n=5) 80-101 85
heparin plasma(n=5) 97-105 101
sodium citrate plasma(n=5) 92-99 95

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的促性腺激素释放激素(GnRH)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中促性腺激素释放激素(GnRH)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 83-98% 96-105% 89-98% 78-97%
EDTA plasma(n=5) 95-104% 98-105% 78-104% 96-105%
heparin plasma(n=5) 88-102% 95-103% 87-94% 79-95%
sodium citrate plasma(n=5) 93-102% 81-101% 83-97% 90-98%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠、检测溶液A）标准品或样本50μL及磁珠10μL，加检测溶液A50μL，
    37°C酶标板振荡器孵育60分钟；
3. 磁吸洗板3次；
4. 加检测溶液B100μL，37°C振动孵育30分钟；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋涡震荡2分钟后读数。

实验原理

将促性腺激素释放激素(GnRH)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠、标记VEGFA，标记的促性腺激素释放激素(GnRH)等多因子检测试剂盒(流式荧光发光法)和未标记的促性腺激素释放激素(GnRH)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体竞争结合，然后将未结合物洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的促性腺激素释放激素(GnRH)等多因子检测试剂盒(流式荧光发光法)呈负相关。

赠品

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
RPA843Hu01 促性腺激素释放激素(GnRH)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
CPA843Hu23 促性腺激素释放激素(GnRH)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
CPA843Hu24 促性腺激素释放激素(GnRH)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
SPA843Hu01 促性腺激素释放激素(GnRH)合成多肽 Immunogen; Blocking Peptide.
CPA843Hu22 促性腺激素释放激素(GnRH)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
CPA843Hu21 促性腺激素释放激素(GnRH)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA843Hu02 促性腺激素释放激素(GnRH)多克隆抗体 WB; IHC; ICC; IP.
PAA843Hu01 促性腺激素释放激素(GnRH)多克隆抗体 WB; IHC; ICC; IP.
LAA843Hu81 促性腺激素释放激素(GnRH)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA843Hu71 促性腺激素释放激素(GnRH)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA843Hu21 促性腺激素释放激素(GnRH)单克隆抗体 WB; IHC; ICC; IP.
MAA843Hu22 促性腺激素释放激素(GnRH)单克隆抗体 WB; IHC; ICC; IP.
CEA843Hu 促性腺激素释放激素(GnRH)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA843Hu 促性腺激素释放激素(GnRH)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA843Hu11 促性腺激素释放激素(GnRH)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Mol Pharmacol. Maternal exposure to dioxin imprints sexual immaturity of the pups through fixing the status of the reduced expression of hypothalamic gonadotropin-releasing … [Pubmed:24132183]
Journal of Sports Science & Medicine Heavy Resistance Training and Supplementation With the Alleged Testosterone Booster Nmda has No Effect on Body Composition, Muscle Performance, and Serum Hormones Associated With the Hypothalamo-Pituitary-Gonadal Axis in Resistance-Trained Males [Pubmed:24570624]
Environmental Research Perfluorooctane sulfonate effects on the reproductive axis in adult male rats [Pubmed:25171141]
Toxicology Letters Possible role of serotonin and neuropeptide Y on the disruption of the reproductive axis activity by perfluorooctane sulfonate [Pubmed:25623392]
Toxicol Sci Chronic exposure of female mice to an environmental level of perfluorooctane sulfonate suppresses estrogen synthesis through reduced histone H3K14 acetylation of the StAR promoter leading to deficits in follicular development and ovulation [PubMed: 26358002]
Int J Mol Sci. Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in Mice [Pubmed:26771609]
Oral Presentations OP0019-HPR Effectiveness of A Progressive Resistance Strength Program on Hand Osteoarthritis: A Randomised Controlled Trial [60.1.abstract]
Oral Presentations OP0021-HPR Sleep Disorders in Patients with Rheumatoid Arthritis and Relationship with Disease Activity [60.3.abstract]
Toxicological Sciences Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring [pubmed:27803384]
Chronobiology of the immune reponse OP0020 The Association of Gonadotropin-Releasing Hormone and Cytokines in Rheumatoid Arthritis [content:75]
Environmental Science & Technology Pyrethroid Insecticide Cypermethrin Accelerates Pubertal Onset in Male Mice via Disrupting Hypothalamic-Pituitary-Gonadal Axis [pubmed:28731686]
Toxicological Sciences Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons [10.1093:toxsci]
GENES Nectin-like molecule 2, a necessary sexual maturation regulator, participates in congenital hypogonadotropic hypogonadism [Pubmed: 32535046]
BioMed Research International Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress [Pubmed: 32685488]
JOURNAL OF ETHNOPHARMACOLOGY Zihuai recipe alleviates cyclophosphamide-induced diminished ovarian reserve via suppressing PI3K/AKT-mediated apoptosis [33422655]
J Virol Zika virus infection in the ovary induces continuously elevated progesterone level and compromises conception in interferon α/β receptor-deficient mice [34730391]
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