组胺(HA)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Histamine (HA) ,etc. by FLIA (Flow Luminescence Immunoassay)
Histamin
（注：单次混测多因子不超过8个指标 ）
- 编号LMA927Ge
- 物种Pan-species (General，通用) 相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长1.5h
- 检测范围0.1-100ng/mL
- 灵敏度最小可检测剂量小于等于0.033 ng/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 8指标数 7指标数 6指标数 5指标数 4指标数 3指标数 2指标数1指标数
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产品包装(模拟)
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产品包装(模拟)
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测组胺(HA)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的组胺(HA)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 80-95 | 89 |
| EDTA plasma(n=5) | 94-101 | 97 |
| heparin plasma(n=5) | 90-97 | 94 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的组胺(HA)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中组胺(HA)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 81-104% | 93-102% | 82-95% | 81-103% |
| EDTA plasma(n=5) | 80-101% | 97-105% | 89-97% | 79-94% |
| heparin plasma(n=5) | 79-89% | 81-101% | 80-105% | 85-99% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠、检测溶液A）标准品或样本50μL及磁珠10μL，加检测溶液A50μL，
37°C酶标板振荡器孵育60分钟；
3. 磁吸洗板3次；
4. 加检测溶液B100μL，37°C振动孵育30分钟；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋涡震荡2分钟后读数。
实验原理
将组胺(HA)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠、标记VEGFA，标记的组胺(HA)等多因子检测试剂盒(流式荧光发光法)和未标记的组胺(HA)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体竞争结合，然后将未结合物洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的组胺(HA)等多因子检测试剂盒(流式荧光发光法)呈负相关。
赠品
增值服务
相关产品
| 编号 | 适用物种：Pan-species (General，通用) | 应用(仅供研究使用，不用于临床诊断！) |
| CPA927Ge41 | 组胺(HA)人血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| CPA927Ge11 | 组胺(HA)牛血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| CPA927Ge21 | 组胺(HA)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| PAA927Ge01 | 组胺(HA)多克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| LAA927Ge71 | 组胺(HA)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAA927Ge81 | 组胺(HA)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| MAA927Ge21 | 组胺(HA)单克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| CEA927Ge | 组胺(HA)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA927Ge | 组胺(HA)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA927Ge | 组胺(HA)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA927Ge11 | 组胺(HA)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It(ELISA Kit) Yourself" |
参考文献
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| Neurogastroenterology & Motility | Expression of inducible nitric oxide synthase in mast cells contributes to the reCavia (Guinea pig )lation of inflammatory cytokines in irritable bowel syndrome with diarrhea [Pubmed:26940641] |
| Italian Journal of Animal Science | Induction of immune responses and allergic reactions in piglets by injecting glycinin [doi:1828051X.2016.1144488] |
| Int J Clin Exp Pathol | Effects of Dectin-1 on the mast cells in allergic conjunctivitis and its underlying mechanism [files:ijcep0019305.pdf] |
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| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat Model [Pubmed: 30781605] |
| Royal Society Open Science | Metabolomics analysis of baicalin on ovalbumin-sensitized allergic rhinitis rats [] |
| International Journal of Pharmaceutical Sciences and Research | MOTION SICKNESS INDUCES PHYSIOLOGICAL AND NEURONAL ALTERATIONS IN MOUSE MODEL [] |
| Allergy | Artemisia annua sublingual immunotherapy for seasonal allergic rhinitis: a randomized controlled trial [Pubmed: 32030780] |
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