白介素1α(IL1a)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Interleukin 1 Alpha (IL1a) ,etc. by FLIA (Flow Luminescence Immunoassay)
IL1-A; IL-1α; IL1F1; Preinterleukin 1 Alpha; Hematopoietin-1; Pro-Interleukin-1-Alpha; Interleukin-1 Family Member 1
(注:单次混测多因子不超过8个指标 )
- 编号LMA071Bo
- 物种Bos taurus; Bovine (Cattle,牛) 相同的名称,不同的物种。
- 实验方法双抗夹心
- 反应时长3.5h
- 检测范围0.98-1000pg/mL
- 灵敏度最小可检测剂量小于等于0.327 pg/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 8指标数 7指标数 6指标数 5指标数 4指标数 3指标数 2指标数1指标数
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特异性
本试剂盒用于检测白介素1α(IL1a)等多因子检测试剂盒(流式荧光发光法),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的白介素1α(IL1a)等多因子检测试剂盒(流式荧光发光法)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。
样本 | 回收率范围(%) | 平均回收率(%) |
serum(n=5) | 80-97 | 90 |
EDTA plasma(n=5) | 92-105 | 97 |
heparin plasma(n=5) | 94-105 | 98 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%
线性
在定值血清及血浆样本内加入适量的白介素1α(IL1a)等多因子检测试剂盒(流式荧光发光法),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中白介素1α(IL1a)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
样本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 79-95% | 85-99% | 78-95% | 92-99% |
EDTA plasma(n=5) | 91-105% | 85-103% | 97-105% | 93-102% |
heparin plasma(n=5) | 79-98% | 99-105% | 87-95% | 83-101% |
稳定性
经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备;
2. 加样(标准品、样本、磁珠)标准品或样本100μL及磁珠10μL,
37°C酶标板振荡器孵育90分钟;
3. 磁吸甩干,加检测溶液A100μL,37°C酶标板振荡器孵育60分钟;
4. 磁吸洗板3次;
5. 加检测溶液B100μL,37°C振动孵育30分钟;
6. 磁吸洗板3次;
7. 加鞘液100μL,旋涡震荡2分钟后读数。
实验原理
将白介素1α(IL1a)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠,制成固相载体,向微孔中分别加入标准品或标本以及磁珠,其中的白介素1α(IL1a)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合,然后加入生物素化的白介素1α(IL1a)等多因子检测试剂盒(流式荧光发光法)抗体,将未结合的生物素化抗体洗净后,加入PE标记的亲和素,再次彻底洗涤后即可上机读数。MFI值和样品中的白介素1α(IL1a)等多因子检测试剂盒(流式荧光发光法)呈正相关。
赠品
增值服务
相关产品
编号 | 适用物种:Bos taurus; Bovine (Cattle,牛) | 应用(仅供研究使用,不用于临床诊断!) |
RPA071Bo01 | 白介素1α(IL1a)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA071Bo01 | 白介素1α(IL1a)多克隆抗体 | WB; IHC; ICC; IP. |
MAA071Bo21 | 白介素1α(IL1a)单克隆抗体 | WB; IHC; ICC; IP. |
SEA071Bo | 白介素1α(IL1a)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA071Bo | 白介素1α(IL1a)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
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