乳酸脱氢酶(LDH)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Lactate Dehydrogenase (LDH) ,etc. by FLIA (Flow Luminescence Immunoassay)
（注：单次混测多因子不超过8个指标 ）
- 编号LMB864Ra
- 物种Rattus norvegicus (Rat，大鼠) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3.5h
- 检测范围0.05-50ng/mL
- 灵敏度最小可检测剂量小于等于0.017 ng/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 8指标数 7指标数 6指标数 5指标数 4指标数 3指标数 2指标数1指标数
- 价格 ¥ 2702 ¥ 2806 ¥ 2962 ¥ 3170 ¥ 3377 ¥ 3689 ¥ 4157 ¥ 5196 添加到价格计算器 计算器
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
-
产品包装(模拟)
-
产品包装(模拟)
-
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测乳酸脱氢酶(LDH)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的乳酸脱氢酶(LDH)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 90-97 | 94 |
| EDTA plasma(n=5) | 89-96 | 92 |
| heparin plasma(n=5) | 93-104 | 96 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的乳酸脱氢酶(LDH)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中乳酸脱氢酶(LDH)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 91-105% | 84-104% | 89-97% | 81-101% |
| EDTA plasma(n=5) | 81-97% | 83-97% | 81-91% | 90-97% |
| heparin plasma(n=5) | 87-102% | 80-95% | 81-92% | 80-94% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。
实验原理
将乳酸脱氢酶(LDH)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的乳酸脱氢酶(LDH)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的乳酸脱氢酶(LDH)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的乳酸脱氢酶(LDH)等多因子检测试剂盒(流式荧光发光法)呈正相关。
赠品
增值服务
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| PAB864Ra01 | 乳酸脱氢酶(LDH)多克隆抗体 | WB; IHC; ICC; IP. |
| MAB864Ra21 | 乳酸脱氢酶(LDH)单克隆抗体 | WB; IHC; ICC; IP. |
| SEB864Ra | 乳酸脱氢酶(LDH)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB864Ra | 乳酸脱氢酶(LDH)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Neuroscience Research | Hydrogen sulfide antagonizes homocysteine-induced neurotoxicity in PC12 cells [PubMed: 20674619] |
| Clinical and Experimental Pharmacology and Physiology | Formaldehyde induces neurotoxicity to PC12 cells involving inhibition of paraoxonase-1 expression and activity [PubMed: 21261675] |
| Neurochemistry International | Hydrogen sulfide prevents formaldehyde-induced neurotoxicity to PC12 cells by attenuation of mitochondrial dysfunction and pro-apoptotic potential [ScienceDirect: S0197018612001428] |
| ostaglandins Leukot Essent Fatty Acids. | Docosahexaenoic and eicosapentaenoic acid supplementation does not exacerbate oxidative stress or intravascular haemolysis in homozygous sickle cell patients. [Pubmed: 24095588] |
| Journal of Diabetes and its Complications | Nerve growth factor rescues diabetic mice heart after ischemia/reperfusion injury via up-regulation of the TRPV1 receptor [Pubmed:25650182] |
| Cancer Science & Therapy | An Additional risk of Lung Cancer from Recurrent Exposure to Ethyl Carbamate (EC) in BALB/C Mice [Open-Access: 1000375] |
| Journal of Antimicrobial Chemotherapy | NLF20: an antimicrobial peptide with therapeutic potential against invasive Pseudomonas aeruginosa infection [PubMed: 26503666] |
| American Journal of Physology Heart and Circulatory Physiology | Dysfunction of mitochondria and deformed gap junctions in the heart of IL-18-deficient mice [Pubmed:27288439] |
| Translational Research | Proteomic analysis of fine-needle aspiration in differential diagnosis of thyroid nodules [Pubmed:27172385] |
| Experimental Cell Research | UpreCavia (Guinea pig )lation of CBS/H2S system contributes to asymmetric dimethylarginine-triggered protection against the neurotoxicity of glutamate to PC12 cells by inhibiting NOS/NO pathway [science:S0014482716301598] |
| Blood Cells Mol Dis | Transgenic sickle cell trait mice do not exhibit abnormal thermoreCavia (Guinea pig )latory and stress responses to heat shock exposure [Pubmed:27282581] |
| PLoS One. | Outer Balloon Ligation Increases Success Rate of Ischemia-Reperfusion Injury Model in Mice. [pubmed:27907155] |
| Scientific Reports | Adiponectin protects the rats liver against chronic intermittent hypoxia induced injury throughAMP-activated protein kinase pathway. [pubmed:27678302] |
| Experimental Biology and Medicine | Ameliorating effect of anti-Alzheimer’s drugs on the bidirectional association between type 2 diabetes mellitus and Alzheimer’s disease [pubmed:28534431] |
| Cancer Science | A Novel Potential Strategy for Enhancing Antigen Presentation of Human Hepatocellular Carcinoma Cells Propagated ex-vivo Using a Ribonuclease Enzyme … [Scholarena:Source] |
| Molecular Medicine Reports | Protective effects of N (2)‑L‑alanyl‑L‑glutamine mediated by the JAK2/STAT3 signaling pathway on myocardial ischemia reperfusion [Pubmed:29393473] |
| Molecular Medicine Reports | Hydrogen‑rich solution against myocardial injury and aquaporin expression via the PI3K/Akt signaling pathway during cardiopulmonary bypass in rats [Pubmed:29956781] |
| Oncology Letters | A novel potential effective strategy for enhancing the antitumor immune response in breast cancer patients using a viable cancer cell‑dendritic cell‑based vaccine [Pubmed:29928442] |
| Experimental Allergy and Immunology | Osteopontin protects against pneumococcal infection in a murine model of allergic airway inflammation [Pubmed: 30362569] |
| Scientific Reports | Low plasma haptoglobin is a risk factor for life-threatening childhood severe malarial anemia and not an exclusive consequence of hemolysis [Pubmed: 30510258] |
| Molecular Medicine Reports | Differentially expressed lncRNAs, miRNAs and mRNAs with associated ceRNA networks in a mouse model of myocardial ischemia/reperfusion injury [Pubmed: 32705277] |
| Cell Transplant | Extracellular Vesicles Derived from Human Umbilical Cord Mesenchymal Stromal Cells Protect Cardiac Cells Against Hypoxia/Reoxygenation Injury by Inhibiting … [Pubmed: 32864999] |
| Nanomaterials | Biological Safety and Biodistribution of Chitosan Nanoparticles [Pubmed: 32340313] |
| Journal of Renal Injury Prevention | Prevention of kidney injury after myocardial ischemia reperfusion is achievable with short-term protein restriction [] |
| clinical and translational medicine | Milk fat globule EGF factor 8 restores mitochondrial function via integrin©\medicated activation of the FAK©\STAT3 signaling pathway in acute pancreatitis [33634976] |
| Biomed Res Int | Factors Affecting the Incidence, Progression, and Severity of COVID-19 in Type 1 Diabetes Mellitus [34840966] |
| Cell Cycle | Improvement of autophagic flux mediates the protection of hydrogen sulfide against arecoline-elicited neurotoxicity in PC12 cells [Pubmed:35316162] |
| Biomed Res Int | A Famous Chinese Medicine Formula: Yinhuo Decoction Antagonizes the Damage of Corticosterone to PC12 Cells and Improves Depression by Regulating … [Pubmed:35281603] |
| Int Immunopharmacol | LncRNA KCNQ1OT1 attenuates myocardial injury induced by hip fracture via regulating of miR-224-3p/GATA4 axis [Pubmed:35217336] |