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乳酸脱氢酶(LDH)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Lactate Dehydrogenase (LDH) ,etc. by FLIA (Flow Luminescence Immunoassay)

(注:单次混测多因子不超过8个指标 )

  • 乳酸脱氢酶(LDH)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • 乳酸脱氢酶(LDH)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测乳酸脱氢酶(LDH)等多因子检测试剂盒(流式荧光发光法),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的乳酸脱氢酶(LDH)等多因子检测试剂盒(流式荧光发光法)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 90-97 94
EDTA plasma(n=5) 89-96 92
heparin plasma(n=5) 93-104 96

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的乳酸脱氢酶(LDH)等多因子检测试剂盒(流式荧光发光法),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中乳酸脱氢酶(LDH)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 91-105% 84-104% 89-97% 81-101%
EDTA plasma(n=5) 81-97% 83-97% 81-91% 90-97%
heparin plasma(n=5) 87-102% 80-95% 81-92% 80-94%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备;
2. 加样(标准品、样本、磁珠)标准品或样本100μL及磁珠10μL,
    37°C酶标板振荡器孵育90分钟;
3. 磁吸甩干,加检测溶液A100μL,37°C酶标板振荡器孵育60分钟;
4. 磁吸洗板3次;
5. 加检测溶液B100μL,37°C振动孵育30分钟;
6. 磁吸洗板3次;
7. 加鞘液100μL,旋涡震荡2分钟后读数。

实验原理

将乳酸脱氢酶(LDH)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠,制成固相载体,向微孔中分别加入标准品或标本以及磁珠,其中的乳酸脱氢酶(LDH)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合,然后加入生物素化的乳酸脱氢酶(LDH)等多因子检测试剂盒(流式荧光发光法)抗体,将未结合的生物素化抗体洗净后,加入PE标记的亲和素,再次彻底洗涤后即可上机读数。MFI值和样品中的乳酸脱氢酶(LDH)等多因子检测试剂盒(流式荧光发光法)呈正相关。

赠品

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
PAB864Ra01 乳酸脱氢酶(LDH)多克隆抗体 WB; IHC; ICC; IP.
MAB864Ra21 乳酸脱氢酶(LDH)单克隆抗体 WB; IHC; ICC; IP.
SEB864Ra 乳酸脱氢酶(LDH)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB864Ra 乳酸脱氢酶(LDH)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Neuroscience Research Hydrogen sulfide antagonizes homocysteine-induced neurotoxicity in PC12 cells [PubMed: 20674619]
Clinical and Experimental Pharmacology and Physiology Formaldehyde induces neurotoxicity to PC12 cells involving inhibition of paraoxonase-1 expression and activity [PubMed: 21261675]
Neurochemistry International Hydrogen sulfide prevents formaldehyde-induced neurotoxicity to PC12 cells by attenuation of mitochondrial dysfunction and pro-apoptotic potential [ScienceDirect: S0197018612001428]
ostaglandins Leukot Essent Fatty Acids. Docosahexaenoic and eicosapentaenoic acid supplementation does not exacerbate oxidative stress or intravascular haemolysis in homozygous sickle cell patients. [Pubmed: 24095588]
Journal of Diabetes and its Complications Nerve growth factor rescues diabetic mice heart after ischemia/reperfusion injury via up-regulation of the TRPV1 receptor [Pubmed:25650182]
Cancer Science & Therapy An Additional risk of Lung Cancer from Recurrent Exposure to Ethyl Carbamate (EC) in BALB/C Mice [Open-Access: 1000375]
Journal of Antimicrobial Chemotherapy NLF20: an antimicrobial peptide with therapeutic potential against invasive Pseudomonas aeruginosa infection [PubMed: 26503666]
American Journal of Physology Heart and Circulatory Physiology Dysfunction of mitochondria and deformed gap junctions in the heart of IL-18-deficient mice [Pubmed:27288439]
Translational Research Proteomic analysis of fine-needle aspiration in differential diagnosis of thyroid nodules [Pubmed:27172385]
Experimental Cell Research UpreCavia (Guinea pig )lation of CBS/H2S system contributes to asymmetric dimethylarginine-triggered protection against the neurotoxicity of glutamate to PC12 cells by inhibiting NOS/NO pathway [science:S0014482716301598]
Blood Cells Mol Dis Transgenic sickle cell trait mice do not exhibit abnormal thermoreCavia (Guinea pig )latory and stress responses to heat shock exposure [Pubmed:27282581]
PLoS One. Outer Balloon Ligation Increases Success Rate of Ischemia-Reperfusion Injury Model in Mice. [pubmed:27907155]
Scientific Reports Adiponectin protects the rats liver against chronic intermittent hypoxia induced injury throughAMP-activated protein kinase pathway. [pubmed:27678302]
Experimental Biology and Medicine Ameliorating effect of anti-Alzheimer’s drugs on the bidirectional association between type 2 diabetes mellitus and Alzheimer’s disease [pubmed:28534431]
Cancer Science A Novel Potential Strategy for Enhancing Antigen Presentation of Human Hepatocellular Carcinoma Cells Propagated ex-vivo Using a Ribonuclease Enzyme … [Scholarena:Source]
Molecular Medicine Reports Protective effects of N (2)‑L‑alanyl‑L‑glutamine mediated by the JAK2/STAT3 signaling pathway on myocardial ischemia reperfusion [Pubmed:29393473]
Molecular Medicine Reports  Hydrogen‑rich solution against myocardial injury and aquaporin expression via the PI3K/Akt signaling pathway during cardiopulmonary bypass in rats [Pubmed:29956781]
Oncology Letters A novel potential effective strategy for enhancing the antitumor immune response in breast cancer patients using a viable cancer cell‑dendritic cell‑based vaccine [Pubmed:29928442]
Experimental Allergy and Immunology Osteopontin protects against pneumococcal infection in a murine model of allergic airway inflammation [Pubmed: 30362569]
Scientific Reports Low plasma haptoglobin is a risk factor for life-threatening childhood severe malarial anemia and not an exclusive consequence of hemolysis [Pubmed: 30510258]
Molecular Medicine Reports Differentially expressed lncRNAs, miRNAs and mRNAs with associated ceRNA networks in a mouse model of myocardial ischemia/reperfusion injury [Pubmed: 32705277]
Cell Transplant Extracellular Vesicles Derived from Human Umbilical Cord Mesenchymal Stromal Cells Protect Cardiac Cells Against Hypoxia/Reoxygenation Injury by Inhibiting … [Pubmed: 32864999]
Nanomaterials Biological Safety and Biodistribution of Chitosan Nanoparticles [Pubmed: 32340313]
Journal of Renal Injury Prevention Prevention of kidney injury after myocardial ischemia reperfusion is achievable with short-term protein restriction []
clinical and translational medicine Milk fat globule EGF factor 8 restores mitochondrial function via integrin©\medicated activation of the FAK©\STAT3 signaling pathway in acute pancreatitis [33634976]
Biomed Res Int Factors Affecting the Incidence, Progression, and Severity of COVID-19 in Type 1 Diabetes Mellitus [34840966]
Cell Cycle Improvement of autophagic flux mediates the protection of hydrogen sulfide against arecoline-elicited neurotoxicity in PC12 cells [Pubmed:35316162]
Biomed Res Int A Famous Chinese Medicine Formula: Yinhuo Decoction Antagonizes the Damage of Corticosterone to PC12 Cells and Improves Depression by Regulating … [Pubmed:35281603]
Int Immunopharmacol LncRNA KCNQ1OT1 attenuates myocardial injury induced by hip fracture via regulating of miR-224-3p/GATA4 axis [Pubmed:35217336]
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