肌肉生长抑制素(MSTN)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Myostatin (MSTN) ,etc. by FLIA (Flow Luminescence Immunoassay)
GDF8; Growth Differentiation Factor 8
(注:单次混测多因子不超过8个指标 )
- 编号LMB653Hu
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 实验方法竞争抑制
- 反应时长1.5h
- 检测范围48.83-50000pg/mL
- 灵敏度最小可检测剂量小于等于16.277 pg/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 下载 英文说明书 中文说明书
- 规格 8指标数 7指标数 6指标数 5指标数 4指标数 3指标数 2指标数1指标数
- 价格 ¥ 2490 ¥ 2586 ¥ 2729 ¥ 2921 ¥ 3112 ¥ 3399 ¥ 3830 计算器 ¥ 4788 添加到价格计算器
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
本试剂盒用于检测肌肉生长抑制素(MSTN)等多因子检测试剂盒(流式荧光发光法),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的肌肉生长抑制素(MSTN)等多因子检测试剂盒(流式荧光发光法)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。
样本 | 回收率范围(%) | 平均回收率(%) |
serum(n=5) | 79-95 | 87 |
EDTA plasma(n=5) | 83-93 | 86 |
heparin plasma(n=5) | 78-104 | 84 |
sodium citrate plasma(n=5) | 83-102 | 97 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%
线性
在定值血清及血浆样本内加入适量的肌肉生长抑制素(MSTN)等多因子检测试剂盒(流式荧光发光法),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中肌肉生长抑制素(MSTN)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
样本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 86-101% | 82-97% | 88-97% | 79-97% |
EDTA plasma(n=5) | 93-102% | 89-96% | 88-101% | 86-96% |
heparin plasma(n=5) | 78-92% | 91-98% | 91-105% | 79-102% |
sodium citrate plasma(n=5) | 78-97% | 83-99% | 78-95% | 92-101% |
稳定性
经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备;
2. 加样(标准品、样本、磁珠、检测溶液A)标准品或样本50μL及磁珠10μL,加检测溶液A50μL,
37°C酶标板振荡器孵育60分钟;
3. 磁吸洗板3次;
4. 加检测溶液B100μL,37°C振动孵育30分钟;
5. 磁吸洗板3次;
6. 加鞘液100μL,旋涡震荡2分钟后读数。
实验原理
将肌肉生长抑制素(MSTN)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠,制成固相载体,向微孔中分别加入标准品或标本以及磁珠、标记VEGFA,标记的肌肉生长抑制素(MSTN)等多因子检测试剂盒(流式荧光发光法)和未标记的肌肉生长抑制素(MSTN)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体竞争结合,然后将未结合物洗净后,加入PE标记的亲和素,再次彻底洗涤后即可上机读数。MFI值和样品中的肌肉生长抑制素(MSTN)等多因子检测试剂盒(流式荧光发光法)呈负相关。
赠品
增值服务
相关产品
编号 | 适用物种:Homo sapiens (Human,人) | 应用(仅供研究使用,不用于临床诊断!) |
APB653Hu01 | 肌肉生长抑制素(MSTN)活性蛋白 | Cell culture; Activity Assays. |
EPB653Hu61 | 肌肉生长抑制素(MSTN)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPB653Hu01 | 肌肉生长抑制素(MSTN)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APB653Hu61 | 肌肉生长抑制素(MSTN)活性蛋白 | Cell culture; Activity Assays. |
CPB653Hu21 | 肌肉生长抑制素(MSTN)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
PAB653Hu08 | 肌肉生长抑制素(MSTN)多克隆抗体 | WB; IHC; ICC; IP. |
PAB653Hu06 | 肌肉生长抑制素(MSTN)多克隆抗体 | WB; IHC; ICC; IP. |
PAB653Hu01 | 肌肉生长抑制素(MSTN)多克隆抗体 | WB; IHC; ICC; IP. |
LAB653Hu71 | 肌肉生长抑制素(MSTN)多克隆抗体(生物素标记) | WB; IHC; ICC. |
MAB653Hu23 | 肌肉生长抑制素(MSTN)单克隆抗体 | WB; IHC; ICC; IP. |
MAB653Hu22 | 肌肉生长抑制素(MSTN)单克隆抗体 | WB; IHC; ICC; IP. |
MAB653Hu21 | 肌肉生长抑制素(MSTN)单克隆抗体 | WB; IHC; ICC; IP. |
SEB653Hu | 肌肉生长抑制素(MSTN)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CEB653Hu | 肌肉生长抑制素(MSTN)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCB653Hu | 肌肉生长抑制素(MSTN)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMB653Hu | 肌肉生长抑制素(MSTN)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSB653Hu11 | 肌肉生长抑制素(MSTN)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
Clinical Medicine and Diagnostics | Anti-Myostatin Reduces Bone Mineral Loss in Ovariectomized Rats [Sapub:Source] |
Nutr Metab (Lond) | Short-term, high-fat diet accelerates disuse atrophy and protein degradation in a muscle-specific manner in mice [PubMed: 26539241] |
International Journal of Cancer | Tumor inoculation site affects the development of cancer cachexia and muscle wasting [PubMed: 26016447] |
Connect Tissue Res | Induction of transient tenogenic phenotype of high density cultured human dermal fibroblasts [PubMed: 25748814] |
PLoS One | Higher Plasma Myostatin Levels in Cor Pulmonale Secondary to Chronic Obstructive Pulmonary Disease [Pubmed:26998756] |
Calcif Tissue Int. | The Effect of Vibration Treatments Combined with Teriparatide or Strontium Ranelate on Bone Healing and Muscle in Ovariectomized Rats [Pubmed:27272029] |
Archives of medical research | Irisin and Myostatin Levels in Patients with Graves' Disease. [pubmed:27986127] |
Calcified Tissue International | Effect of Strontium Ranelate on the Muscle and Vertebrae of Ovariectomized Rats [pubmed:29242963] |
Scientific Reports | Compensatory anabolic signaling in the sarcopenia of experimental chronic arthritis. [pubmed:28740214] |
Journal of Animal Science | Fermented corn-soybean meal elevated IGF1 levels in grower-finisher pigs [Pubmed: 30203098] |
European Journal of Internal Medicine | Predictive value of serum myostatin for the severity and clinical outcome of heart failure [Pubmed: 31056368] |
J Cell Physiol | Resistance training‐induced muscle hypertrophy is mediated by TGF‐β1‐Smad signaling pathway in male Wistar rats [Pubmed: 31960436] |
NEPHROLOGY DIALYSIS TRANSPLANTATION | Myostatin and muscle atrophy during chronic kidney disease [Pubmed: 32974666] |
Oxid Med Cell Longev | Gender-Specific Behaviour in Obesity Stages I-II: Imbalance of Aminothiol Status and Adipomyokine Profile in Subjects with Different Insulin Resistance … [34868459] |