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内脂素(VF)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Visfatin (VF) ,etc. by FLIA (Flow Luminescence Immunoassay)

Nampt; NAmPRTase; PBEF; PBEF1; Pre-B-Cell Colony Enhancing Factor; Nicotinamide Phosphoribosyltransferase

(注:单次混测多因子不超过8个指标 )

  • 内脂素(VF)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • 内脂素(VF)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测内脂素(VF)等多因子检测试剂盒(流式荧光发光法),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的内脂素(VF)等多因子检测试剂盒(流式荧光发光法)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 83-93 90
EDTA plasma(n=5) 79-98 83
heparin plasma(n=5) 82-94 89

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的内脂素(VF)等多因子检测试剂盒(流式荧光发光法),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中内脂素(VF)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 92-101% 87-96% 94-101% 87-96%
EDTA plasma(n=5) 80-90% 92-101% 94-102% 78-91%
heparin plasma(n=5) 98-105% 82-103% 83-101% 80-103%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备;
2. 加样(标准品、样本、磁珠)标准品或样本100μL及磁珠10μL,
    37°C酶标板振荡器孵育90分钟;
3. 磁吸甩干,加检测溶液A100μL,37°C酶标板振荡器孵育60分钟;
4. 磁吸洗板3次;
5. 加检测溶液B100μL,37°C振动孵育30分钟;
6. 磁吸洗板3次;
7. 加鞘液100μL,旋涡震荡2分钟后读数。

实验原理

将内脂素(VF)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠,制成固相载体,向微孔中分别加入标准品或标本以及磁珠,其中的内脂素(VF)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合,然后加入生物素化的内脂素(VF)等多因子检测试剂盒(流式荧光发光法)抗体,将未结合的生物素化抗体洗净后,加入PE标记的亲和素,再次彻底洗涤后即可上机读数。MFI值和样品中的内脂素(VF)等多因子检测试剂盒(流式荧光发光法)呈正相关。

赠品

相关产品

编号 适用物种:Cavia (Guinea pig ,豚鼠) 应用(仅供研究使用,不用于临床诊断!)
RPA638Gu01 内脂素(VF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA638Gu01 内脂素(VF)多克隆抗体 WB; IHC; ICC; IP.
MAA638Gu21 内脂素(VF)单克隆抗体 WB; IHC; ICC; IP.
SEA638Gu 内脂素(VF)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA638Gu 内脂素(VF)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Brazilian Journal of Biomoricity EFFECT OF 8 WEEKS ENDURANCE TRAINING ON PLASMA VISFATIN IN MIDDLE-AGED MEN [Brjb: source]
Diabetes Research and Clinical Practice The relationship between visfatin and HOMA-IR in hypertensive patients, and the effect of antihypertensive drugs on visfatin and HOMA-IR in hypertensive patients with insulin resistance [ScienceDirect: S0168822711002956]
J Physiol Pharmacol. Early-effect of bariatric surgery (Scopinaro method) on intestinal hormones and adipokines in insulin resistant Wistar rat. [Pubmed: 24304571]
Endocr J. CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytes [Pubmed:25168658]
Exp Ther Med. Plasma visfatin levels and mRNA expression of visfatin in peripheral blood mononuclear cells and peripheral blood monocyte-derived macrophages from normal weight females with polycystic ovary syndrome [Pubmed:Pmc3991522]
Eur J Cancer Prev. Visfatin derived from ascites promotes ovarian cancer cell migration through Rho/ROCK signaling-mediated actin polymerization. [Pubmed:25055182]
Genome Visfatin −948G/T and resistin −420C/Gpolymorphisms in Egyptian type 2 diabetic patients with and without cardiovascular diseases [Nrcresearchpress:Source]
PLOS ONE Antidiabetic Effect of Galantamine: Novel Effect for a Known Centrally Acting Drug [PubMed: 26262991]
Alcoholism and Drug Addiction Changes in concentration of visfatin during four weeks of inpatient treatment of alcohol dependent males [Science: Article]
Cytokine Variation in blood levels of hormones in obese patients following weight reduction induced by endoscopic and surgical bariatric therapies [PubMed: 26539806]
Genome Genetic Variations in Visfatin and Resistin Genes in Egyptian Type 2 Diabetic Patients With and Without Complications [PubMed: 25120107]
Acta Histochemica Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokines [science:S0065128116301234]
Endocrine, Metabolic & Immune Disorders-Drug Targets Changes in Serum Nampt Levels and Its Significances in Diabetic Nephropathy Patients-The Potential Role of Nampt in T2DM with Diabetic Nephropathy. [pubmed:28699485]
Endocrine Connections Post-chemotherapy serum anti-müllerian hormone level predicts ovarian function recovery [Pubmed:30012648]
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Contraception Hyperinsulinemic-euglycemic clamp over the first year of use of depot-medroxyprogesterone acetate as a contraceptive [Pubmed:29665358]
Cranio - Journal of Craniomandibular Practice Analysis of synovial fluid visfatin level in temporomandibular joint disorders [Pubmed:29385907]
Nutrire Resistin and visfatin concentrations are related to central obesity and inflammation in Brazilian children [10.1186:s41110-018-0060-7]
DNA and Cell Biology Transcriptional Regulation of NAMPT Gene by Glycogen Synthase Kinase 3β in Goat Adipocytes [Pubmed: 30615481]
Jundishapur Journal of Chronic Disease Care The Effect of 8 Weeks Aquatic Exercise Program on Plasma Visfatin Level and Some Blood Factors in Obese Men []
Biomarkers in Heart Failure and Associated Diseases Adipokines as Biomarkers in Health and Disease []
Journal of Translational Medicine Plasma concentration and expression of adipokines in epicardial and subcutaneous adipose tissue are associated with impaired left ventricular filling pattern [Pubmed: 31533725]
clinical neurology and neurosurgery Relationshıp of spontaneous subarachnoid haemorrhage and cerebral aneurysm to serum Visfatin and Nesfatin-1 levels [Pubmed: 32311618]
The Horizon of Medical Sciences Effects of 10-Week Concurrent Training on Insulin Resistance and the Serum Levels of Vaspin and Visfatin in Overweight Females []
EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY Different Ratios of Corn and Coconut Oil Blends in High©\Fat Diets Influence Fat Deposition without Altering Metabolic Biomarkers in Male Rats []
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