Ⅰ型胶原交联氨基端肽(NTXI)检测试剂盒(酶联免疫吸附试验法,小样本)
Mini Samples ELISA Kit for Cross Linked N-Telopeptide Of Type I Collagen (NTXI)
NTX-I; NTX1
- 编号MEA639Mu
- 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长3h
- 检测范围2.47-200ng/mL
- 灵敏度最小可检测剂量小于等于0.89ng/mL.
- 样本类型Serum, plasma, urine and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 3268 ¥ 4668 ¥ 21006 ¥ 39678 ¥ 326760
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
-
产品包装(模拟)
-
产品包装(模拟)
-
实验结果图
-
标准曲线图
-
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测Ⅰ型胶原交联氨基端肽(NTXI)检测试剂盒(酶联免疫吸附试验法,小样本)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的Ⅰ型胶原交联氨基端肽(NTXI)检测试剂盒(酶联免疫吸附试验法,小样本)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 83-95 | 91 |
| EDTA plasma(n=5) | 85-104 | 95 |
| heparin plasma(n=5) | 94-102 | 98 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的Ⅰ型胶原交联氨基端肽(NTXI)检测试剂盒(酶联免疫吸附试验法,小样本)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中Ⅰ型胶原交联氨基端肽(NTXI)检测试剂盒(酶联免疫吸附试验法,小样本)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-101% | 99-105% | 78-89% | 90-99% |
| EDTA plasma(n=5) | 98-105% | 81-103% | 94-102% | 94-105% |
| heparin plasma(n=5) | 92-101% | 78-101% | 88-103% | 86-98% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）25µL后，立即加入检测溶液A25µL，
37°C孵育1小时；
3. 甩干，洗板3次；
4. 加检测溶液B50µL，37°C孵育30分钟；
5. 洗板5次；
6. 加TMB底物50µL，37°C孵育10－20分钟；
7. 加终止液25µL，立即450nm读数。
实验原理
本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将Ⅰ型胶原交联氨基端肽(NTXI)检测试剂盒(酶联免疫吸附试验法,小样本)单克隆抗体包被微孔板，制成固相载体，往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本)，待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物，然后加入HRP标记的亲和素，经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。待测标本浓度越高，标记抗原和抗体的结合就越受到抑制，显色愈浅。显色的深浅与酶量呈正相关，而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度（O.D.值），计算样品浓度。
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| CEA639Mu | Ⅰ型胶原交联氨基端肽(NTXI)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA639Mu | Ⅰ型胶原交联氨基端肽(NTXI)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA639Mu | Ⅰ型胶原交联氨基端肽(NTXI)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA639Mu11 | Ⅰ型胶原交联氨基端肽(NTXI)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Nutrition | Dyslipidemic high-fat diet affects adversely bone metabolism in mice associated with impaired antioxidant capacity [ScienceDirect: S0899900709004717] |
| Bone | Differential mRNA expression profiles in proximal tibia of aged rats in response to ovariectomy and low-Ca diet [ScienceDirect: S875632820800776X] |
| Journal of Orthopaedic Research | Promotion of bone formation by naringin in a titanium particle‐induced diabetic murine calvarial osteolysis model [Wiley: Source] |
| The Journal of Clinical Endocrinology & Metabolism | A Mediterranean Diet Enriched with Olive Oil Is Associated with Higher Serum Total Osteocalcin Levels in Elderly Men at High Cardiovascular Risk [PubMed: PMC3462931] |
| Acta Paediatr.? | Biochemical markers of bone resorption are present in human milk: implications for maternal and neonatal bone metabolism [Pubmed:25109232] |
| PLoS One. | An NMR Metabolomic Study on the Effect of Alendronate in Ovariectomized Mice [Pubmed:25184758] |
| J Bone Miner Res | Strontium Ranelate Reduces the Fracture Incidence in a Growing Mouse Model of Osteogenesis Imperfecta [PubMed: 26679066] |
| Journal of Functional Foods | Comparative effects of brown and golden flaxseeds on body composition, inflammation and bone remodelling biomarkers in perimenopausal overweight women [S1756464617301561] |
| Archives of Oral Biology | Impact of a chronic smoking habit on the osteo-immunoinflammatory mediators in the peri-implant fluid of clinically healthy dental implants. [pubmed:27328151] |
| Journal of Periodontology | Impact of a triclosan‐containing toothpaste during the progression of experimental peri‐implant mucositis: Clinical parameters and local pattern of osteo … [Pubmed:29520826] |
| Clinical oral implants research | Triclosan‐containing fluoride toothpaste on clinical parameters and osteo‐inflammatory mediators when applied in a stent during experimental peri‐implant mucositis … [Pubmed: 30659666] |
| Molecular Therapy-Nucleic Acids | AAV-anti-miR-214 prevents collapse of the femoral head in osteonecrosis by regulating osteoblast and osteoclast activities [Pubmed: 31739209] |
| Bioengineering (Basel) | Material-Dependent Formation and Degradation of Bone Matrix—Comparison of Two Cryogels [Pubmed: 32517006] |
| BioMed Research International | Comparable Effects of Strontium Ranelate and Alendronate Treatment on Fracture Reduction in a Mouse Model of Osteogenesis Imperfecta [33506016] |
| PHARMACEUTICAL BIOLOGY | Effects of icariin on the fracture healing in young and old rats and its mechanism [34511043] |
| Evid Based Complement Alternat Med | Gujiansan Ameliorates Avascular Necrosis of the Femoral Head by Regulating Autophagy via the HIF-1α/BNIP3 Pathway [34512780] |
