Ⅰ型前胶原氨基端原肽(PINP)检测试剂盒(酶联免疫吸附试验法,小样本)
Mini Samples ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)
P1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide
- 编号MEA957Mu
- 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长3h
- 检测范围3.70-300ng/mL
- 灵敏度最小可检测剂量小于等于1.44ng/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 3520 ¥ 5028 ¥ 22626 ¥ 42738 ¥ 351960
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测Ⅰ型前胶原氨基端原肽(PINP)检测试剂盒(酶联免疫吸附试验法,小样本)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的Ⅰ型前胶原氨基端原肽(PINP)检测试剂盒(酶联免疫吸附试验法,小样本)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 96-104 | 101 |
| EDTA plasma(n=5) | 79-90 | 82 |
| heparin plasma(n=5) | 80-102 | 84 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的Ⅰ型前胶原氨基端原肽(PINP)检测试剂盒(酶联免疫吸附试验法,小样本)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中Ⅰ型前胶原氨基端原肽(PINP)检测试剂盒(酶联免疫吸附试验法,小样本)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 95-103% | 93-101% | 86-101% | 92-99% |
| EDTA plasma(n=5) | 83-102% | 96-105% | 83-101% | 85-99% |
| heparin plasma(n=5) | 89-103% | 78-102% | 93-101% | 86-95% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）25µL后，立即加入检测溶液A25µL，
37°C孵育1小时；
3. 甩干，洗板3次；
4. 加检测溶液B50µL，37°C孵育30分钟；
5. 洗板5次；
6. 加TMB底物50µL，37°C孵育10－20分钟；
7. 加终止液25µL，立即450nm读数。
实验原理
本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将Ⅰ型前胶原氨基端原肽(PINP)检测试剂盒(酶联免疫吸附试验法,小样本)单克隆抗体包被微孔板，制成固相载体，往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本)，待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物，然后加入HRP标记的亲和素，经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。待测标本浓度越高，标记抗原和抗体的结合就越受到抑制，显色愈浅。显色的深浅与酶量呈正相关，而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度（O.D.值），计算样品浓度。
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA957Mu01 | Ⅰ型前胶原氨基端原肽(PINP)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| SPA957Mu01 | Ⅰ型前胶原氨基端原肽(PINP)合成多肽 | Immunogen; Blocking Peptide. |
| CPA957Mu21 | Ⅰ型前胶原氨基端原肽(PINP)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| PAA957Mu01 | Ⅰ型前胶原氨基端原肽(PINP)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA957Mu08 | Ⅰ型前胶原氨基端原肽(PINP)多克隆抗体 | WB; IHC; ICC; IP. |
| SEA957Mu | Ⅰ型前胶原氨基端原肽(PINP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| IEA957Mu | Ⅰ型前胶原氨基端原肽(PINP)检测试剂盒(酶联免疫吸附试验法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA957Mu | Ⅰ型前胶原氨基端原肽(PINP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA957Mu | Ⅰ型前胶原氨基端原肽(PINP)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA957Mu | Ⅰ型前胶原氨基端原肽(PINP)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA957Mu11 | Ⅰ型前胶原氨基端原肽(PINP)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Trials | Is there benefit in optimising heart failure treatment in over-80 year-old patients? (HF-80 study): study protocol for a randomized controlled trial. [Trials: 1745-6215] |
| Clinical Biochemistry | Measurement of aminoterminal propeptide of type I procollagen (PINP) in serum [ScienceDirect: S0009912012001543] |
| Therapeutic Apheresis and Dialysis | Low molecular weight iron dextran increases fibroblast growth factor-23 concentration, together with parathyroid hormone decrease in hemodialyzed patients [PubMed: 22458393] |
| International Journal of Clinical & Experimental Pathology | IL4 and IL-17A provide a Th2/Th17-polarized inflammatory milieu in favor of TGF-β1 to induce bronchial epithelial-mesenchymal transition (EMT) [PubMed: PMC3726963] |
| Osteoporosis International | PINP as a biological response marker during teriparatide treatment for osteoporosis [Pubmed: 24599274] |
| Clinical Cancer Research | Zoledronic acid has differential anti-tumour activity in the pre-and post-menopausal bone microenvironment in vivo [Aacrjournals: Source] |
| Endocrinology | Oxytocin Reverses Ovariectomy-Induced Osteopenia and Body Fat Gain [Endocrine: Source] |
| general endocrinology | Oxytocin reverse ovariectomy-induced Osteoper and body fat gain [Pubmed:24506069] |
| J Neurol Sci. | The effect of topiramate and lamotrigine on rat bone mass, structure and metabolism. [Pubmed:24629477] |
| Bone. | Neurofibromin inactivation impairs osteocyte development in Nf1Prx1 and Nf1Col1 mouse models [Pubmed:24947449] |
| Journal of Materials Science: Materials in Medicine | Phosphatidylethanolamine biomimetic coating increases mesenchymal stem cell osteoblastogenesis [Pubmed:24980874] |
| Springerplus. | Effects of de-escalated bisphosphonate therapy on bone turnover biomarkers in breast cancer patients with bone metastases [Pubmed:25332877] |
| Pharmacology | Effect of Mirtazapine on Rat Bone Tissue after Orchidectomy [PubMed: 25871861] |
| frontiers in endocrinology | Oxytocin reverses osteoporosis in a sex-dependent manner [PubMed: 26042090] |
| MERIT RESEARCH JOURNALS | Changes in Bone Metabolism Months after Laparoscopic Morbidly Obese Pre-menopausal Women [Mms: Content] |
| Heart Rhythm. | Prognostic value of collagen turnover biomarkers in cardiac resynchronization therapy: A subanalysis of the TRUST CRT randomized trial population [Pubmed:26776557] |
| BMJ Open Sport Exerc Med | Procollagen markers in microdialysate can predict patient outcome after Achilles tendon rupture [content:2] |
| Bibliothekssystem Universität Hamburg | Bilaterale Kommunikation zwischen Osteoblasten und Osteoklasten [volltexte:2016] |
| Inflammation Research | Fibrosis of extracellular matrix is related to the duration of the disease but is unrelated to the dynamics of collagen metabolism in dilated cardiomyopathy [pubmed:27516211] |
| Endocrine | Bone turnover response is linked to both acute and established metabolic changes in ultra-marathon runners [pubmed:27422791] |
| Heart & Vessels | Left ventricular reverse remodeling is not related to biopsy-detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR [pubmed:28004175] |
| journal of science and medicine in sport | The effect of calcium and vitamin D supplementation on bone health of male Jockeys. [pubmed:27568072] |
| Combined aerobic and resistance training improves bone health of female cancer survivors [S2352187216300389] | |
| Cellular Physiology and Biochemistry | Renal Denervation Attenuates Multi-Organ Fibrosis and Improves Vascular Remodeling in Rats with Transverse Aortic Constriction Induced Cardiomyopathy [pubmed:27889753] |
| Epilepsy Research | The effect of lamotrigine and phenytoin on bone turnover and bone strength: A prospective study in Wistar rats [pubmed:27838501] |
| Sci Rep. | Sprint Interval Training Induces A Sexual Dimorphism but does not Improve Peak Bone Mass in Young and Healthy Mice. [pubmed:28303909] |
| Metabolism. | Low periostin levels in adult patients with Langerhans cell histiocytosis are independently associated with the disease activity. [pubmed:28521873] |
| Heart and vessels | Erratum to: Effects on bone metabolism markers and arterial stiffness by switching to rivaroxaban from warfarin in patients with atrial fibrillation [pubmed:28314975] |
| SCIENCE & TECHNOLOGY | A Study Protocol: Spinal Morphology, Physical Performance, Quality of Life and Biochemical Markers in Adults at Risk of Osteoporotic Fractures [59f7c85aaca272607e2d8d7a] |
| Knee Surg Sports Traumatol Arthrosc | Achilles tendon rupture healing is enhanced by intermittent pneumatic compression upregulating collagen type I synthesis [pubmed:28668970] |
| Method for the treatment of cardiovascular fibrosis [patents:US9594083] | |
| Clinical Cases in Mineral and Bone Metabolism | Periostin and sclerostin levels in juvenile Paget's disease [pubmed:29263750] |
| Journal of Clinical Research in Pediatric Endocrinology | Congenital Hypothyroidism and Bone Remodeling Cycle [pubmed:27840329] |
| Biomarkers Medicial | Relationship of biomarkers of extracellular matrix with myocardial function in Type 2 diabetes mellitus [pubmed:28685602] |
| Heart and Vessels | Left ventricular reverse remodeling is not related to biopsy‑detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR [10.1007/s00380-016-0930-y] |
| BioMed Research International | Optimization of the Time Window of Interest in Ovariectomized Imprinting Control Region Mice for Antiosteoporosis Research [pubmed:29119115] |
| Cytokine | 12-month patterns of serum markers of collagen synthesis, transforming growth factor and connective tissue growth factor are similar in new-onset and chronic dilated cardiomyopathy in patients both with and without cardiac fibrosis. [pubmed:28460256] |
| Advanced Healthcare Materials | Prognostic value of fibrosis-related markers in dilated cardiomyopathy: A link between osteopontin and cardiovascular events. [pubmed:29120858] |
| Bone. | The key role of proinflammatory cytokines, matrix proteins, RANKL/OPG and Wnt/β-catenin in bone healing of hip arthroplasty patients [pubmed:29129760] |
| Cell Death And Differentiation | TSC1 regulates osteoclast podosome organization and bone resorption through mTORC1 and Rac1/Cdc42 [Pubmed:29358671] |
| Nutrients | Specific Collagen Peptides Improve Bone Mineral Density and Bone Markers in Postmenopausal Women—A Randomized Controlled Study [Pubmed:29337906] |
| Osteoporosis International | Two-year cortical and trabecular bone loss in CKD-5D: biochemical and clinical predictors [Pubmed:28993865] |
| Journal of Nutrition | Cholecalciferol Supplementation Promotes Bone Turnover in Chinese Adults with Vitamin D Deficiency [Pubmed:29897564] |
| European Journal of Pharmacology | The effect of levetiracetam on rat bone mineral density, bone structure and biochemical markers of bone metabolism [Pubmed:29428468] |
| Journal of Clinical Densitometry | Geometric and mechanical bone response to a multidisciplinary weight loss intervention in adolescents with obesity: The ADIBOX study [Pubmed:30076009] |
| Hernia | Circulating matrix metalloproteinases and procollagen propeptides in inguinal hernia [Pubmed:29484522] |
| Pharmacology | Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive Rats [Pubmed:29898457] |
| Nature | Coupling of bone resorption and formation by RANKL reverse signalling [Pubmed: 30185903] |
| European Journal of Medical Research | Biomechanical and histological analyses of the fracture healing process after direct or prolonged reduction [Pubmed: 30180907] |
| Sensors | Membrane-Based Microwave-Mediated Electrochemical Immunoassay for the In Vitro, Highly Sensitive Detection of Osteoporosis-Related Biomarkers [Pubmed: 30181433] |
| Trials | Structured diet and exercise guidance in pregnancy to improve health in women and their offspring: study protocol for the Be Healthy in Pregnancy (BHIP) … [Pubmed: 30567604] |
| International Journal of Cancer | Effect of induction therapy with lenalidomide, doxorubicin, and dexamethasone on bone remodeling and angiogenesis in newly diagnosed multiple myeloma [Pubmed: 30650184] |
| Bone | Rapamycin improves bone mass in high-turnover osteoporosis with iron accumulation through positive effects on osteogenesis and angiogenesis [Pubmed: 30610968] |
| Revista de Chimie -Bucharest- | Quantification of N-Terminal Procollagen I Propeptide in Patients With Occlusal Trauma Induced by Prosthetic Factors [] |
| PLOS ONE | Effect of antibiotic infused calcium sulfate/hydroxyapatite (CAS/HA) insets on implant-associated osteitis in a femur fracture model in mice [Pubmed: 30870491] |
| Scientific Reports | Low fibrosis biomarker levels predict cardiac resynchronization therapy response [Pubmed: 30988339] |
| Current Developments in Nutrition | Changes in Calciotropic Hormones and Bone Markers During Pregnancy in Response to a Nutrition+ Exercise Intervention in the Be Healthy in Pregnancy RCT (P11 … [Pubmed: 31224444] |
| Stem Cell Research & Therapy | Low concentration flufenamic acid enhances osteogenic differentiation of mesenchymal stem cells and suppresses bone loss by inhibition of the NF-κB … [Pubmed: 31324207] |
| Bone | The long noncoding RNA Crnde regulates osteoblast proliferation through the Wnt/β-catenin signaling pathway in mice [Pubmed: 31622775] |
| International Journal of Basic and Clinical Endocrinology | Expression of prolactin receptors in the duodenum, kidneys and skeletal system during physiological and sulpiride-induced hyperprolactinaemia [Pubmed: 30143940] |
| Bone | Mice lacking plastin-3 display a specific defect of cortical bone acquisition [Pubmed: 31678489] |
| International journal of molecular sciences | Differences in Osteoimmunological Biomarkers Predictive of Psoriatic Arthritis among a Large Italian Cohort of Psoriatic Patients [Pubmed: 31717649] |
| Journal of Bone and Mineral Research | BMP9 reduces bone loss in ovariectomized mice by dual regulation of bone remodeling [Pubmed: 31914211] |
| ESC Heart Failure | Outcome in German and South African peripartum cardiomyopathy cohorts associates with medical therapy and fibrosis markers [Pubmed: 32064780] |
| International Journal of Medical Sciences | Hypoalbuminemia differently affects the serum bone turnover markers in hemodialysis patients [Pubmed: 31839746] |
| JOURNAL OF BONE AND MINERAL RESEARCH | Piezo1 Inactivation in Chondrocytes Impairs Trabecular Bone Formation [Pubmed: 33180356] |
| JOURNAL OF THROMBOSIS AND THROMBOLYSIS | Extracellular matrix collagen biomarkers levels in patients with chronic thromboembolic pulmonary hypertension [Pubmed: 33175289] |
| INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | Development of a novel polysaccharide-based iron oxide nanoparticle to prevent iron accumulation-related osteoporosis by scavenging reactive oxygen … [Pubmed: 33049237] |
| journal of bone and mineral research | Mice carrying a ubiquitous R235W mutation of Wnt1 display a bone‐specific phenotype [Pubmed: 32369212] |
| Endokrynologia Polska | FGF23 and primary hyperparathyroidism: is there a link? [Pubmed: 32598019] |
| Journal of Orthopaedic Translation | Qu Feng Zhi Tong capsule increases mechanical properties of cortical bone in ovariectomized rats [] |
| Research square | 4-Hexylresorcinol Inhibits Osteoclastogenesis by Suppressing NF‐κB Signaling Pathway and Reverses Bone Loss in Ovariectomized Mice [] |
| 淫羊藿水提物及提取药渣的抗骨质疏松药效比较研究 [] | |
| Pamukkale Tıp Dergisi | Postmenapozal Tip-2 diyabetes mellitus' lu hastalarda osteoporoza bağlı kırık riskinin ve kemik Turnoveri'nin değerlendirilmesi [] |
| International Journal of Pharmaceutical and Clinical Research | Bone Turnover Markers in Osteogenesis Imperfecta and Effect of Bisphosphonate Treatment: First Egyptian Study [] |
| Science Advances | A biomimetically hierarchical polyetherketoneketone scaffold for osteoporotic bone repair [33310848] |
| Rheumatology | Proinflammatory and bone protective role of calcitonin gene-related peptide alpha in collagen antibody-induced arthritis [33221885] |
| Nutrients | Low Energy Availability with and without a High-Protein Diet Suppresses Bone Formation and Increases Bone Resorption in Men: A Randomized Controlled Pilot ¡ [33671093] |
| J Bone Miner Res | Lgr4 promotes aerobic glycolysis and differentiation in osteoblasts via the canonical Wnt/¦Â©\catenin pathway [33950533] |
| Phytomedicine | Randomized, double-blind, placebo-controlled trial to examine the safety, pharmacokinetics and effects of Epimedium prenylflavonoids, on bone specific alkaline … [34352588] |
| Nutrients | The Effect of Daily Methylsulfonylmethane (MSM) Consumption on High-Density Lipoprotein Cholesterol in Healthy Overweight and Obese Adults: A Randomized … [34684621] |
| PLoS One | Osteoblast-specific inactivation of p53 results in locally increased bone formation [34793446] |
| Bone Res | The WNT1G177C mutation specifically affects skeletal integrity in a mouse model of osteogenesis imperfecta type XV [34759273] |
| Nutrients | The Effect of Caloric Restriction with and without n-3 PUFA Supplementation on Bone Turnover Markers in Blood of Subjects with Abdominal Obesity: A Randomized … [34578973] |
| Chronobiol Int | Night shift work and osteoporosis-bone turnover markers among female blue-collar workers in Poland [Pubmed:35139709] |
| Cancers (Basel) | Spine Metastases in Immunocompromised Mice after Intracardiac Injection of MDA-MB-231-SCP2 Breast Cancer Cells [Pubmed:35158823] |
| Cell Death Discov | BMP9 reduces age-related bone loss in mice by inhibiting osteoblast senescence through Smad1-Stat1-P21 axis [Pubmed:35523787] |
| Experimental and Molecular Medicine | Foxf2 represses bone formation via Wnt2b/β-catenin signaling [Pubmed:35668101] |
| Bioactive Materials | Osteoporotic bone recovery by a bamboo-structured bioceramic with controlled release of hydroxyapatite nanoparticles [Pubmed:35386445] |
| Journal of Orthopaedic Translation | Puerarin specifically disrupts osteoclast activation via blocking integrin-β3 Pyk2/Src/Cbl signaling pathway [Pubmed:35228997] |
| Bioactive Materials | Construction of a magnesium hydroxide/graphene oxide/hydroxyapatite composite coating on Mg–Ca–Zn–Ag alloy to inhibit bacterial infection and promote … [Pubmed:35585377] |
| Journal of Orthopaedic Translation | Exercised accelerated the production of muscle-derived kynurenic acid in skeletal muscle and alleviated the postmenopausal osteoporosis through the Gpr35 … [] |
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