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Toll样受体2(TLR2)检测试剂盒(酶联免疫吸附试验法,小样本)

Mini Samples ELISA Kit for Toll Like Receptor 2 (TLR2)

CD282; TIL4; Toll/interleukin-1 receptor-like protein 4

  • Toll样受体2(TLR2)检测试剂盒(酶联免疫吸附试验法,小样本) 产品包装(模拟)
  • Toll样受体2(TLR2)检测试剂盒(酶联免疫吸附试验法,小样本) 产品包装(模拟)
  • Toll样受体2(TLR2)检测试剂盒(酶联免疫吸附试验法,小样本) 实验结果图
  • MEA663Hu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测Toll样受体2(TLR2)检测试剂盒(酶联免疫吸附试验法,小样本),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)25µL,37°C温育1小时;
3. 吸弃,加检测溶液A25µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B25µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物25µL,37°C孵育10-20分钟;
8. 加终止液20µL,立即450nm读数。

实验原理

将Toll样受体2(TLR2)检测试剂盒(酶联免疫吸附试验法,小样本)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的Toll样受体2(TLR2)检测试剂盒(酶联免疫吸附试验法,小样本)与连接于固相载体上的抗体结合,然后加入生物素化的Toll样受体2(TLR2)检测试剂盒(酶联免疫吸附试验法,小样本)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的Toll样受体2(TLR2)检测试剂盒(酶联免疫吸附试验法,小样本)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APA663Hu04 Toll样受体2(TLR2)活性蛋白 Cell culture; Activity Assays.
APA663Hu02 Toll样受体2(TLR2)活性蛋白 Cell culture; Activity Assays.
RPA663Hu02 Toll样受体2(TLR2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA663Hu01 Toll样受体2(TLR2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
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RPA663Hu04 Toll样受体2(TLR2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA663Hu01 Toll样受体2(TLR2)多克隆抗体 WB; IHC; ICC; IP.
PAA663Hu02 Toll样受体2(TLR2)多克隆抗体 WB; IHC; ICC; IP.
PAA663Hu04 Toll样受体2(TLR2)多克隆抗体 WB; IHC; ICC; IP.
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LAA663Hu81 Toll样受体2(TLR2)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA663Hu71 Toll样受体2(TLR2)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA663Hu25 Toll样受体2(TLR2)单克隆抗体 WB; IHC; ICC; IP.
MAA663Hu22 Toll样受体2(TLR2)单克隆抗体 WB; IHC; ICC; IP.
RAA663Hu21 Toll样受体2(TLR2)重组抗体 WB; IF; ICC; IHC; IP; FCM.
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MAA663Hu24 Toll样受体2(TLR2)单克隆抗体 WB; IHC; ICC; IP.
MAA663Hu23 Toll样受体2(TLR2)单克隆抗体 WB; IHC; ICC; IP.
FAA663Hu02 抗Toll样受体2(TLR2)单克隆抗体 Flow cytometry.
FAA663Hu04 抗Toll样受体2(TLR2)单克隆抗体 FCM
LAA663Hu72 Toll样受体2(TLR2)单克隆抗体(生物素标记) WB; IHC; ICC.
SEA663Hu Toll样受体2(TLR2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA663Hu Toll样受体2(TLR2)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
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PSA663Hu01 Toll样受体2(TLR2)抗体对 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA663Hu01 Toll样受体2(TLR2)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

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PLoS One Baicalein Reduces Airway Injury in Allergen and IL-13 Induced Airway Inflammation [PubMed: PMC3639905]
Clinical and Developmental Immunology TLR2 in Pleural Fluid Is Modulated by Talc Particles during Pleurodesis [PubMed: PMC3529880]
Advances in Virology The Possible Role of TLR2 in Chronic Hepatitis B Patients with Precore Mutation [Pubmed: 24187552]
J Matern Fetal Neonatal Med. Cervical and vaginal fluid soluble Toll-like receptor 2 in pregnancies complicated by preterm prelabor rupture of membranes [Pubmed:25028801]
Respiratory Virology and Immunogenicity Advances in Experimental Medicine and Biology Cytokines and Toll-Like Receptors in the Immune Response to Influenza Vaccination [Pubmed:25248345]
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Int J Dermatol. Membrane and soluble Toll‐like receptor 2 in patients with psoriasis treated by Goeckerman therapy [Pubmed:25266302]
BMC Immunology The discriminative capacity of soluble Toll-like receptor (sTLR)2 and sTLR4 in inflammatory diseases [Pubmed:25406630]
Pathol Biol (Paris) Cathelicidin related antimicrobial peptide, laminin, Toll-like receptors and chemokines levels in experimental hypersensitivity pneumonitis in mice [PubMed: 25834936]
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Perfusion Pentraxin 3 and other inflammatory biomarkers related to atrial fibrillation in cardiac surgery [pubmed:27852958]
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CHILD`S HEALTH Prospective study on the significance of mucosal barrier dysfunction markers in assessing the effectiveness of the new strategy for treating food hypersensitivity in … [10.22141:2224-0551.13.2.2018.129548]
Neurological Sciences Vinpocetine regulates levels of circulating TLRs in Parkinson's disease patients [Pubmed: 30315378]
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