Toll样受体2(TLR2)检测试剂盒(酶联免疫吸附试验法,小样本)
Mini Samples ELISA Kit for Toll Like Receptor 2 (TLR2)
CD282; TIL4; Toll/interleukin-1 receptor-like protein 4
- 编号MEA663Hu
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围0.312-20ng/mL
- 灵敏度最小可检测剂量小于等于0.121ng/mL.
- 样本类型saliva,tissue homogenates, cell lysates,cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2512 ¥ 3588 ¥ 16146 ¥ 30498 ¥ 251160
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测Toll样受体2(TLR2)检测试剂盒(酶联免疫吸附试验法,小样本)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）25µL，37°C温育1小时；
3. 吸弃，加检测溶液A25µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B25µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物25µL，37°C孵育10-20分钟；
8. 加终止液20µL，立即450nm读数。
实验原理
将Toll样受体2(TLR2)检测试剂盒(酶联免疫吸附试验法,小样本)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的Toll样受体2(TLR2)检测试剂盒(酶联免疫吸附试验法,小样本)与连接于固相载体上的抗体结合，然后加入生物素化的Toll样受体2(TLR2)检测试剂盒(酶联免疫吸附试验法,小样本)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的Toll样受体2(TLR2)检测试剂盒(酶联免疫吸附试验法,小样本)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| APA663Hu04 | Toll样受体2(TLR2)活性蛋白 | Cell culture; Activity Assays. |
| APA663Hu02 | Toll样受体2(TLR2)活性蛋白 | Cell culture; Activity Assays. |
| RPA663Hu02 | Toll样受体2(TLR2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA663Hu01 | Toll样受体2(TLR2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA663Hu03 | Toll样受体2(TLR2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA663Hu04 | Toll样受体2(TLR2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA663Hu01 | Toll样受体2(TLR2)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA663Hu02 | Toll样受体2(TLR2)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA663Hu04 | Toll样受体2(TLR2)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA663Hu03 | Toll样受体2(TLR2)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA663Hu81 | Toll样受体2(TLR2)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAA663Hu71 | Toll样受体2(TLR2)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAA663Hu25 | Toll样受体2(TLR2)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA663Hu22 | Toll样受体2(TLR2)单克隆抗体 | WB; IHC; ICC; IP. |
| RAA663Hu21 | Toll样受体2(TLR2)重组抗体 | WB; IF; ICC; IHC; IP; FCM. |
| MAA663Hu21 | Toll样受体2(TLR2)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA663Hu24 | Toll样受体2(TLR2)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA663Hu23 | Toll样受体2(TLR2)单克隆抗体 | WB; IHC; ICC; IP. |
| FAA663Hu02 | 抗Toll样受体2(TLR2)单克隆抗体 | Flow cytometry. |
| FAA663Hu04 | 抗Toll样受体2(TLR2)单克隆抗体 | FCM |
| LAA663Hu72 | Toll样受体2(TLR2)单克隆抗体(生物素标记) | WB; IHC; ICC. |
| SEA663Hu | Toll样受体2(TLR2)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA663Hu | Toll样受体2(TLR2)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA663Hu | Toll样受体2(TLR2)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| PSA663Hu01 | Toll样受体2(TLR2)抗体对 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA663Hu01 | Toll样受体2(TLR2)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
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| Chinese Medical Journal | Expression of soluble Toll-like receptors in pleural effusions [CMJ: 2010823483999506297] |
| PLoS One | Baicalein Reduces Airway Injury in Allergen and IL-13 Induced Airway Inflammation [PubMed: PMC3639905] |
| Clinical and Developmental Immunology | TLR2 in Pleural Fluid Is Modulated by Talc Particles during Pleurodesis [PubMed: PMC3529880] |
| Advances in Virology | The Possible Role of TLR2 in Chronic Hepatitis B Patients with Precore Mutation [Pubmed: 24187552] |
| J Matern Fetal Neonatal Med. | Cervical and vaginal fluid soluble Toll-like receptor 2 in pregnancies complicated by preterm prelabor rupture of membranes [Pubmed:25028801] |
| Respiratory Virology and Immunogenicity Advances in Experimental Medicine and Biology | Cytokines and Toll-Like Receptors in the Immune Response to Influenza Vaccination [Pubmed:25248345] |
| Iran J Immunol. | Correlation?between?salivary?Toll like receptor-2?concentration?and early childhood caries. [Pubmed:25265998] |
| Int J Dermatol. | Membrane and soluble Toll‐like receptor 2 in patients with psoriasis treated by Goeckerman therapy [Pubmed:25266302] |
| BMC Immunology | The discriminative capacity of soluble Toll-like receptor (sTLR)2 and sTLR4 in inflammatory diseases [Pubmed:25406630] |
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| Plos One | Soluble Toll-Like Receptors 2 and 4 in Cerebrospinal Fluid of Patients with Acute Hydrocephalus followingAneurysmal Subarachnoid Haemorrhage. [Pubmed:27223696] |
| Open Forum Infect Dis | The Effect of Rosuvastatin on Markers of Immune Activation in Treatment-Naive Human Immunodeficiency Virus-Patients [Pubmed:26835476] |
| Perfusion | Pentraxin 3 and other inflammatory biomarkers related to atrial fibrillation in cardiac surgery [pubmed:27852958] |
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| Acta Orthopaedica | Toll-like receptors and their soluble forms differ in the knee and thumb basal osteoarthritic joints [pubmed:28093922] |
| Biological Markers and Guided Therapy | Features of the Pattern Recognition Receptor TLR-2 Expression and Cytokines IL-2, IL-4 Profile in Children with Food Hypersensitivity [10.12988:bmgt.2018.812] |
| CHILD`S HEALTH | Prospective study on the significance of mucosal barrier dysfunction markers in assessing the effectiveness of the new strategy for treating food hypersensitivity in … [10.22141:2224-0551.13.2.2018.129548] |
| Neurological Sciences | Vinpocetine regulates levels of circulating TLRs in Parkinson's disease patients [Pubmed: 30315378] |
| Biological Markers in Fundamental and Clinical Medicine | PROGNOSTIC ROLE OF IL-4, IL-2 AND TLR-2 FOR PREDICTING OF COURSE OF FOOD ALLERGY IN CHILDREN [Doi: 10.29256/v.02.02.2018.escbm71] |
| Biological Markers in Fundamental and Clinical Medicine | STUDY OF THE ACTION OF CHROMIUM-CONTAINING BIOPREPARATIONS FROM SPIRULINA ON INSULIN ACTIVITY IN RATS [Doi: 10.29256/v.02.02.2018.escbm72] |
| Biomedicine & Pharmacotherapy | Opuntioside, opuntiol and its metallic nanoparticles attenuate adjuvant-induced arthritis: Novel suppressors of Toll-like receptors-2 and-4 [] |
| PLoS One | Aberrant DNA methylation of the toll-like receptors 2 and 6 genes in patients with obstructive sleep apnea [Pubmed: 32069296] |
| Georgian medical news | ASSOCIATIONS BETWEEN EFFICACY OF THE THERAPY AND CIRCADIAN FLUCTUATIONS OF ENDOTHELIAL NITRIC OXIDE SYNTHASE WITH TOLL-LIKE … [Pubmed: 32242853] |
| Medical and Clinical Chemistry | ДОбОвА вАРІАбЕЛЬНІСТЬ РІвНЯ РОзЧИННИХ ТОЛ-пОДІбНИХ РЕцЕпТОРІв 2 в СИРОвАТцІ КРОвІ ЖІНОК, ХвОРИХ НА РЕвМАТОїДНИй АРТРИТ: зв' … [] |
| Sciences of Europe | FEATURES OF MYOCARDIAL REMODELING IN MEN WITH POST-INFARCTION CARDIOSCLEROSIS AND ITS RELATIONSHIP WITH TOLL-LIKE … [] |
| CLINICAL INVESTIGATIONS | Levels of toll-like receptors 2 and 4 in serum of men with postinfarction cardiosclerosis and its relationship with antiphospholipid syndrome components [] |
| Acta Obstet Gynecol Scand | A value of soluble Toll‐like receptor 2 and 4 in vaginally obtained amniotic fluid for the prediction of histological chorioamnionitis [34244993] |
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