角蛋白18(CK18)单克隆抗体
Monoclonal Antibody to Cytokeratin 18 (CK18)
KRT18; CYK18; K18; Keratin 18; Cell proliferation-inducing gene 46 protein; Keratin, type I cytoskeletal 18
- 编号MAB231Hu24
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 来源单抗制备
- 宿主小鼠
- 效价(克隆号)F1
- Ig亚型 IgG1 Kappa
- 纯化方式蛋白A + 蛋白G亲和纯化
- 标记物无标记物
- 免疫原 RPB231Hu01-角蛋白18(CK18)重组蛋白
- 缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
- 性状液体
- 浓度1mg/ml
- 且适物种-
- 应用WB; IHC; ICC; IP.
如果抗体需用于流式细胞术，请参见流式抗体。 - 下载 英文说明书 中文说明书
- 规格 20µl100µl 200µl 1ml 10ml
- 价格 ¥ 643 ¥ 1499 ¥ 2142 ¥ 5355 ¥ 21420
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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FITC staining on IF; Sample: Human HepG2 cell; Primary Ab: 30µg/ml Mouse Anti-Human CK18 Antibody Second Ab: 2µg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu18)
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Western Blot; Sample: Lane1: Rat Liver lysate; Lane2: Human Placenta lysate; Lane3: Rat Small intestine lysate; Lane4: Hela cell lysate Primary Ab: 2μg/ml Mouse Anti-Human KRT18 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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Human MCF7 cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 10µg/ml mouse Anti-human CK18 Monoclonal Antibody (Catalog MAB231Hu24, red histogram) or Isotype control antibody (Catalog IS067-Mu01, green histogram), followed by 1µg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).
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通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对CK18的小鼠单克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别CK18。
用法
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Flow cytometry: 10µg/ml;
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
赠品
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB231Hu01 | 角蛋白18(CK18)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB231Hu02 | 角蛋白18(CK18)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB231Hu03 | 角蛋白18(CK18)多克隆抗体 | WB; IHC; ICC; IP; FCM |
| PAB231Hu01 | 角蛋白18(CK18)多克隆抗体 | WB; IHC; ICC/IF |
| LAB231Hu81 | 角蛋白18(CK18)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAB231Hu71 | 角蛋白18(CK18)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAB231Hu27 | 角蛋白18(CK18)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB231Hu24 | 角蛋白18(CK18)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB231Hu21 | 角蛋白18(CK18)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB231Hu22 | 角蛋白18(CK18)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB231Hu23 | 角蛋白18(CK18)单克隆抗体 | WB; ICC/IF |
| MAB231Hu25 | 角蛋白18(CK18)单克隆抗体 | WB; IHC; ICC; IP. |
| FAB231Hu02 | 抗角蛋白18(CK18)单克隆抗体 | Flow cytometry. |
| FAB231Hu08 | 抗角蛋白18(CK18)单克隆抗体 | Flow cytometry. |
| FAB231Hu06 | 抗角蛋白18(CK18)单克隆抗体 | Flow cytometry. |
| FAB231Hu04 | 抗角蛋白18(CK18)单克隆抗体 | Flow cytometry. |
| SEB231Hu | 角蛋白18(CK18)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB231Hu | 角蛋白18(CK18)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSB231Hu01 | 角蛋白18(CK18)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
| 杂志 | 参考文献 |
| Can J Physiol Pharmacol. | Piceatannol increases the expression of hepatocyte growth factor and IL-10 thereby protecting hepatocytes in thioacetamide-induced liver fibrosis [Pubmed:27186801] |
| J Alzheimers Dis | Cigarette Smoke-Induced Alterations in Frontal White Matter Lipid Profiles Demonstrated by MALDI-Imaging Mass Spectrometry: Relevance to Alzheimer's Disease [Pubmed:26836183] |
| bioscience biotechnology research asia | Malted Barley Improved the Structure and Function of Gastrocnemius Muscle of Hypercholesterolemic Mother Rats and their Offspring [] |
| Biomed Chromatogr | Lipidomic‐based investigation into the therapeutic effects of PPC and BBD on rats with NAFLD [34727379] |
| J Magn Reson Imaging | Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver … [Pubmed:35212074] |
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