α1-抗胰蛋白酶(a1AT)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Alpha-1-Antitrypsin (a1AT)
SERPINA1; SPAAT; A1-AT; PI; Serpin Peptidase Inhibitor,Clade A(Alpha-1 Antiproteinase/AntiTrypsin)Member 1; Alpha-1 protease inhibitor; Short peptide from AAT; Serpin A1
- 编号KSB697Hu01
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围62.5-4,000ng/mL
- 可适灵敏度29.2ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 7182 ¥ 11970 ¥ 21546 ¥ 41895 ¥ 71820
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对α1-抗胰蛋白酶(a1AT)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Homo sapiens (Human,人) | 应用(仅供研究使用,不用于临床诊断!) |
RPB697Hu01 | α1-抗胰蛋白酶(a1AT)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB697Hu01 | α1-抗胰蛋白酶(a1AT)多克隆抗体 | WB; IHC; ICC; IP. |
LAB697Hu71 | α1-抗胰蛋白酶(a1AT)多克隆抗体(生物素标记) | WB; IHC; ICC. |
MAB697Hu24 | α1-抗胰蛋白酶(a1AT)单克隆抗体 | WB; IHC; ICC; IP. |
MAB697Hu25 | α1-抗胰蛋白酶(a1AT)单克隆抗体 | WB; IHC; ICC; IP. |
MAB697Hu23 | α1-抗胰蛋白酶(a1AT)单克隆抗体 | WB; IHC; ICC; IP. |
MAB697Hu29 | α1-抗胰蛋白酶(a1AT)单克隆抗体 | WB; IHC; ICC; IP. |
MAB697Hu27 | α1-抗胰蛋白酶(a1AT)单克隆抗体 | WB; IHC; ICC; IP. |
MAB697Hu26 | α1-抗胰蛋白酶(a1AT)单克隆抗体 | WB; IHC; ICC; IP. |
MAB697Hu21 | α1-抗胰蛋白酶(a1AT)单克隆抗体 | WB; IHC; ICC/IF. |
MAB697Hu22 | α1-抗胰蛋白酶(a1AT)单克隆抗体 | WB; IHC; ICC; IP. |
FAB697Hu02 | 抗α1-抗胰蛋白酶(a1AT)单克隆抗体 | Flow cytometry. |
MEB697Hu | α1-抗胰蛋白酶(a1AT)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SEB697Hu | α1-抗胰蛋白酶(a1AT)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCB697Hu | α1-抗胰蛋白酶(a1AT)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMB697Hu | α1-抗胰蛋白酶(a1AT)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSB697Hu01 | α1-抗胰蛋白酶(a1AT)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
Journal of Biotechnology | Expression and characterization of recombinant human alpha-antitrypsin in transgenic rice seed [PubMed: 23376844] |
Sci Rep | Hepatic steatosis depresses alpha-1-antitrypsin levels in human and rat acute pancreatitis [PubMed: 26634430] |
PLoS One | Gut Microbial Dysbiosis May Predict Diarrhea and Fatigue in Patients Undergoing Pelvic Cancer Radiotherapy: A Pilot Study [PubMed: 25955845] |
Respir Care | Diagnostic Utility of Biomarkers in COPD [PubMed: 26106205] |
Acta Anaesthesiologica Scandinavica | Early prognostic factors in septic shock cancer patients: a prospective study with a proteomic approach [Pubmed:29315472] |
Journal of Clinical Medicine | Urinary Proteomics for the Early Diagnosis of Diabetic Nephropathy in Taiwanese Patients [Pubmed: 30486327] |
Journal of Proteomics | Salivary proteins from dysplastic leukoplakia and oral squamous cell carcinoma and their potential for early detection [Pubmed: 31706945] |
Diagnostics | Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2J [Pubmed: 32585848] |
Pediatrics International | Prenatal diagnosis of maternal serum from mothers carrying β‐thalassemic fetus [34559910] |
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