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α1-抗胰蛋白酶(a1AT)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Alpha-1-Antitrypsin (a1AT)

SERPINA1; SPAAT; A1-AT; PI; Serpin Peptidase Inhibitor,Clade A(Alpha-1 Antiproteinase/AntiTrypsin)Member 1; Alpha-1 protease inhibitor; Short peptide from AAT; Serpin A1

  • α1-抗胰蛋白酶(a1AT)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • α1-抗胰蛋白酶(a1AT)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对α1-抗胰蛋白酶(a1AT)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
RPB697Ra01 α1-抗胰蛋白酶(a1AT)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB697Ra01 α1-抗胰蛋白酶(a1AT)多克隆抗体 WB; IHC; ICC; IP.
LAB697Ra71 α1-抗胰蛋白酶(a1AT)多克隆抗体(生物素标记) WB; IHC; ICC.
MAB697Ra21 α1-抗胰蛋白酶(a1AT)单克隆抗体 WB.
MEB697Ra α1-抗胰蛋白酶(a1AT)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
SEB697Ra α1-抗胰蛋白酶(a1AT)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB697Ra α1-抗胰蛋白酶(a1AT)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSB697Ra01 α1-抗胰蛋白酶(a1AT)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Journal of Biotechnology Expression and characterization of recombinant human alpha-antitrypsin in transgenic rice seed [PubMed: 23376844]
Sci Rep Hepatic steatosis depresses alpha-1-antitrypsin levels in human and rat acute pancreatitis [PubMed: 26634430]
PLoS One Gut Microbial Dysbiosis May Predict Diarrhea and Fatigue in Patients Undergoing Pelvic Cancer Radiotherapy: A Pilot Study [PubMed: 25955845]
Respir Care Diagnostic Utility of Biomarkers in COPD [PubMed: 26106205]
Acta Anaesthesiologica Scandinavica Early prognostic factors in septic shock cancer patients: a prospective study with a proteomic approach [Pubmed:29315472]
Journal of Clinical Medicine Urinary Proteomics for the Early Diagnosis of Diabetic Nephropathy in Taiwanese Patients [Pubmed: 30486327]
Journal of Proteomics Salivary proteins from dysplastic leukoplakia and oral squamous cell carcinoma and their potential for early detection [Pubmed: 31706945]
Diagnostics Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2J [Pubmed: 32585848]
Pediatrics International Prenatal diagnosis of maternal serum from mothers carrying β‐thalassemic fetus [34559910]
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