血管生成素样蛋白4(ANGPTL4)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Angiopoietin Like Protein 4 (ANGPTL4)
ARP4; FIAF; HFARP; NL2; PGAR; Pp1158; Fasting-Induced Adipose Factor; Hepatic Angiopoietin-Related Protein; Hepatic Fibrinogen/Angiopoietin-Related Protein
- 编号KSB019Hu01
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.78-50ng/mL
- 可适灵敏度0.28ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 7182 ¥ 11970 ¥ 21546 ¥ 41895 ¥ 71820
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特异性
试剂盒中的抗体对血管生成素样蛋白4(ANGPTL4)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Homo sapiens (Human,人) | 应用(仅供研究使用,不用于临床诊断!) |
RPB019Hu02 | 血管生成素样蛋白4(ANGPTL4)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPB019Hu01 | 血管生成素样蛋白4(ANGPTL4)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB019Hu01 | 血管生成素样蛋白4(ANGPTL4)多克隆抗体 | WB; IHC; ICC; IP. |
MAB019Hu22 | 血管生成素样蛋白4(ANGPTL4)单克隆抗体 | WB; IHC; ICC; IP. |
SEB019Hu | 血管生成素样蛋白4(ANGPTL4)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB019Hu | 血管生成素样蛋白4(ANGPTL4)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSB019Hu01 | 血管生成素样蛋白4(ANGPTL4)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
The Journal of Biological Chemistry | Angiopoietin-like 4 (Angptl4) Protein Is a Physiological Mediator of Intracellular Lipolysis in Murine Adipocytes [PubMed: PMC3318686] |
J Clin Endocrinol Metab. | Decreased Concentrations of the Lipoprotein Lipase Inhibitor Angiopoietin-Like Protein 4 and Increased Serum Triacylglycerol Are Associated With Increased Neonatal Fat Mass in Pregnant Women With Gestational Diabetes Mellitus [Pubmed: 23744407] |
PloS one | Lack of Relationship between Cord Serum Angiopoietin-Like Protein 4 (ANGPTL4) and Lipolytic Activity in Human Neonates Born by Spontaneous Delivery [Pubmed: 24324678] |
Journal of Hematology & Oncology | Angptl4 is upregulated under inflammatory conditions in the bone marrow of mice, expands myeloid progenitors, and accelerates reconstitution of platelets after myelosuppressive therapy [PubMed: 26054961] |
Gynecol Endocrinol | Examination of angiopoietin-like protein 4, neuropeptide Y, omentin-1 levels of obese and non-obese patients with polycystic ovary syndrome [PubMed: 26291814] |
Molecular Nutrition & Food research | Interaction of Gut Microbiota and High‐sodium, Low‐potassium Diet in Altering Plasma Triglyceride Profiles Revealed by Lipidomics Analysis [Pubmed: 31675161] |
ANNALS OF AGRICULTURAL AND ENVIRONMENTAL MEDICINE | Analysis of novel serum markers of fibrosis and angiogenesis in patients with alcoholic liver cirrhosis [] |
Food Chem Toxicol | Di (2-ethylhexyl) phthalate exposure exacerbates metabolic disorders in diet-induced obese mice [34303773] |
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