β2-微球蛋白(b2M)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Beta-2-Microglobulin (b2M)
BMG; B2MG
- 编号KSA260Ra01
- 物种Rattus norvegicus (Rat,大鼠) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本serum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.312-20ng/mL
- 可适灵敏度0.147ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 7387 ¥ 12312 ¥ 22162 ¥ 43092 ¥ 73872
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对β2-微球蛋白(b2M)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Rattus norvegicus (Rat,大鼠) | 应用(仅供研究使用,不用于临床诊断!) |
RPA260Ra01 | β2-微球蛋白(b2M)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA260Ra01 | β2-微球蛋白(b2M)多克隆抗体 | WB; IHC; ICC; IP. |
LAA260Ra81 | β2-微球蛋白(b2M)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
LAA260Ra71 | β2-微球蛋白(b2M)多克隆抗体(生物素标记) | WB; IHC; ICC. |
MAA260Ra21 | β2-微球蛋白(b2M)单克隆抗体 | WB; IHC; ICC; IP. |
SEA260Ra | β2-微球蛋白(b2M)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA260Ra | β2-微球蛋白(b2M)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSA260Ra01 | β2-微球蛋白(b2M)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
BioMed Research International | The Renal Effects of Vanadate Exposure: Potential Biomarkers and Oxidative Stress as a Mechanism of Functional Renal Disorders—Preliminary Studies [Hindawi: 740105] |
BMC Nephrology | Absence of chloride intracellular channel 4 (CLIC4) predisposes to acute kidney injury but has minimal impact on recovery [Biomedcentral: Source] |
Nature Communications | Mef2C restrains microglial inflammatory response and is lost in brain ageing in an IFN-I-dependent manner. [pubmed:28959042] |
PLoS One | Urine proteome analysis by C18 plate–matrix-assisted laser desorption/ionization time-of-flight mass spectrometry allows noninvasive differential diagnosis … [Pubmed:30024955] |
Acta Anaesthesiologica Scandinavica | Early prognostic factors in septic shock cancer patients: a prospective study with a proteomic approach [Pubmed:29315472] |
Toxicology Mechanisms and Methods | Long-term exposure to crotonaldehyde causes heart and kidney dysfunction through induction of inflammatory and oxidative damage in male Wistar rats [Pubmed: 30426860] |
Journal of Clinical Medicine | Urinary Proteomics for the Early Diagnosis of Diabetic Nephropathy in Taiwanese Patients [Pubmed: 30486327] |
Journal of Proteomics | iTRAQ-based analysis for the identification of MARCH8 targets in human esophageal squamous cell carcinoma [33540066] |
Antioxidants | Diminishment of Nrf2 Antioxidative Defense Aggravates Nephrotoxicity of Melamine and Oxalate Coexposure. Antioxidants 2021, 10, 1464 [34573096] |
Int Arch Occup Environ Health | Biomarkers of cadmium exposure and renal function in estuarine adult villagers [34773507] |
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