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防御素α1(DEFa1)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Defensin Alpha 1, Neutrophil (DEFa1)

DEF1; DEFA2; HNP-1; HP-1; MRS; DEF3; HNP-3; HNP3; HP-3; Defensin,Alpha 1; Myeloid-Related Sequence

  • 防御素α1(DEFa1)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 防御素α1(DEFa1)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对防御素α1(DEFa1)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

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参考文献

杂志 参考文献
PLoS One. Cerebrospinal Fluid Biomarker Candidates Associated with Human WNV Neuroinvasive Disease [Plos: Source]
The prognosis and investigation of a-defensin-1 (hnp-1) influence on morphological changes of Staphylococcus aureus cells by the atomic-force microscopy data [10.14300/mnnc.2017.12086 ISSN - 2073-8137]
Scientific Reports Leukotriene B4 is essential for lung host defence and alpha-defensin-1 production during Achromobacter xylosoxidans infection. [pubmed:29247243]
Comparison of human and mouse fetal intestinal tissues reveals differential maturation timelines. []
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