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纤维介素蛋白(FGL2)检测试剂盒DIY材料(酶联免疫吸附试验法)

试剂盒中的抗体对纤维介素蛋白(FGL2)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

杂志	参考文献
Gut.	Expression of B and T lymphocyte attenuator (BTLA) in macrophages contributes to the fulminant hepatitis caused by murine hepatitis virus strain-3 [PubMed: 22637698]
Hepatogastroenterology.	Plasma fibrinogen-like protein 2 levels in patients with non-alcoholic fatty liver disease. [PubMed: 22024080]
PLoS Pathogens	Programmed Death (PD)-1-Deficient Mice Are Extremely Sensitive to Murine Hepatitis Virus Strain-3 (MHV-3) Infection [PubMed: PMC3131267]
Immunology letters	Tumor necrosis factor α (TNF-α) receptor-I is required for TNF-α-mediated fulminant virus hepatitis caused by murine hepatitis virus strain-3 infection. [Pubmed: 24286726 ]
Immunol Lett.	Tumor necrosis factor α (TNF-α) receptor-I is required for TNF-α-mediated fulminant virus hepatitis caused by murine hepatitis virus strain-3 infection [Pubmed:24286726]
NATURE COMMUNICATIONS	VSIG4 inhibits proinflammatory macrophage activation by reprogramming mitochondrial pyruvate metabolism [pubmed:29109438]
Cancer Medicine	Soluble fibrinogen‐like protein 2 ameliorates acute rejection of liver transplantation in rat via inducing Kupffer cells M2 polarization [Pubmed:29749104]
BMC Infectious Diseases	Soluble fibrinogen-like protein 2 levels in patients with hepatitis B virus-related liver diseases [Pubmed: 30419833]
Int J Gynaecol Obstet	Tandem mass tag©\based quantitative proteomic profiling of the serum of patients with abnormal uterine bleeding associated with copper intrauterine device [33354778]

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编号	适用物种：Mus musculus (Mouse，小鼠)	应用(仅供研究使用，不用于临床诊断！)
RPA512Mu02	纤维介素蛋白(FGL2)重组蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
RPA512Mu01	纤维介素蛋白(FGL2)重组蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
PAA512Mu02	纤维介素蛋白(FGL2)多克隆抗体	WB; IHC; ICC; IP.
PAA512Mu01	纤维介素蛋白(FGL2)多克隆抗体	WB; IHC; ICC; IP.
SEA512Mu	纤维介素蛋白(FGL2)检测试剂盒(酶联免疫吸附试验法)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMA512Mu	纤维介素蛋白(FGL2)等多因子检测试剂盒(流式荧光发光法)	FLIA Kit for Antigen Detection.
KSA512Mu01	纤维介素蛋白(FGL2)检测试剂盒DIY材料(酶联免疫吸附试验法)	Main materials for "Do It (ELISA Kit) Yourself".