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凝溶胶蛋白(GSN)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Gelsolin (GSN)

GS; AGEL; ADF; Brevin; Gelsolin Amyloidosis,Finnish Type; Actin-depolymerizing factor

  • 凝溶胶蛋白(GSN)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 凝溶胶蛋白(GSN)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对凝溶胶蛋白(GS)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

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参考文献

杂志 参考文献
Neurochmical Research Gelsolin in Cerebrospinal Fluid as a Potential Biomarker of Epilepsy [SpringerLink: 3585r8h46q7r0171]
Journal of Neuroinflammation Treatment with gelsolin reduces brain inflammation and apoptotic signaling in mice following thermal injury [BioMed: 17422094]
Biomedical Engineering and Informatics Application of iTRAQ quantitative proteomics in identification of serum biomarkers in breast cancer [IEEE: 6098563]
European Journal of Obstetrics & Gynecology and Reproductive Biology Investigation of the actin scavenging system in pre-eclampsia [Pubmed: 24239294]
The Annals of Thoracic Surgery Proteomic Study Reveals Plasma Protein Changes in Congenital Heart Diseases [Pubmed: 24565402]
BMC Anesthesiol.? The role of plasma gelsolin in cardiopulmonary bypass induced acute lung injury in infants and young children: a pilot study [Pubmed:Pmc4132929]
Cell Biochemistry and Biophysics Gelsolin: Role of a Functional Protein in Mitigating Radiation Injury [Pubmed:25164111]
Neurosci Lett Plasma gelsolin and matrix metalloproteinase 3 as potential biomarkers for Alzheimer disease [PubMed: 25864780]
Proteomics Clinical Applications Comparative mass spectrometric and immunoassay‐based proteome analysis in serum of Duchenne muscular dystrophy patients [Pubmed:26680509]
PROTEOMICS - Clinical Applications Comparative mass spectrometric and immunoassay-based proteome analysis in serum ofDuchenne muscular dystrophy patients. [pubmed:26680509]
Medicina Intensiva Serum gelsolin levels in aneurismal subarachnoid hemorrhage: Preliminary results. [pubmed:28365030]
Journal of Alzheimers Disease Urine-Based Biomarkers for Alzheimer's Disease Identified Through Coupling Computational and Experimental Methods [Pubmed:30040720]
World Journal of Pediatrics Plasma gelsolin level predicts acute kidney injury after cardiopulmonary bypass in infants and young children [Pubmed:29427164]
Biomedicines Production and Secretion of Gelsolin by Both Human Macrophage-and Fibroblast-like Synoviocytes and GSN Modulation in the Synovial Fluid of Patients with Various … [Pubmed:35327525]
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