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结合珠蛋白(Hpt)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Haptoglobin (Hpt)

HP; Hp2-Alpha; Alpha-2-Macroglobulin; Zonulin

  • 结合珠蛋白(Hpt)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 结合珠蛋白(Hpt)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对结合珠蛋白(Hpt)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

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参考文献

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Food and Chemical Toxicology Prolyl endopeptidase-degraded low immunoreactive wheat flour attenuates immune responses in Caco-2 intestinal cells and gluten-sensitized BALB/c mice [Pubmed: 31082461]
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