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70kDa热休克蛋白5(HSPA5)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Heat Shock 70kDa Protein 5 (HSPA5)

MIF2; BIP; GRP78; Immunoglobulin heavy chain-binding protein; Glucose Regulated Protein 78; Binding Immunoglobulin Protein; Endoplasmic reticulum lumenal Ca binding grp78

  • 70kDa热休克蛋白5(HSPA5)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 70kDa热休克蛋白5(HSPA5)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对70kDa热休克蛋白5(HSPA5)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
RPC343Mu02 70kDa热休克蛋白5(HSPA5)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPC343Mu01 70kDa热休克蛋白5(HSPA5)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPC343Mu61 70kDa热休克蛋白5(HSPA5)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPC343Mu03 70kDa热休克蛋白5(HSPA5)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAC343Mu02 70kDa热休克蛋白5(HSPA5)多克隆抗体 WB; IHC; ICC; IP.
PAC343Mu01 70kDa热休克蛋白5(HSPA5)多克隆抗体 WB; IHC; ICC; IP.
LAC343Mu71 70kDa热休克蛋白5(HSPA5)多克隆抗体(生物素标记) WB; IHC; ICC.
MAC343Mu25 70kDa热休克蛋白5(HSPA5)单克隆抗体 WB; IHC; ICC; IP.
MAC343Mu22 70kDa热休克蛋白5(HSPA5)单克隆抗体 WB; IHC; ICC; IP.
MAC343Mu21 70kDa热休克蛋白5(HSPA5)单克隆抗体 WB; IHC; ICC; IP.
MAC343Mu27 70kDa热休克蛋白5(HSPA5)单克隆抗体 WB; IHC; ICC; IP.
MAC343Mu23 70kDa热休克蛋白5(HSPA5)单克隆抗体 WB; IHC; ICC; IP.
MAC343Mu24 70kDa热休克蛋白5(HSPA5)单克隆抗体 WB; IHC; ICC; IP.
SEC343Mu 70kDa热休克蛋白5(HSPA5)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC343Mu 70kDa热休克蛋白5(HSPA5)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMC343Mu 70kDa热休克蛋白5(HSPA5)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSC343Mu01 70kDa热休克蛋白5(HSPA5)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
41 Deficiency of Rac1 Blocks NADPH Oxidase Activation, Inhibits Endoplasmic Reticulum Stress, and Reduces Myocardial Remodeling in a Mouse Model of Type 1 Diabetes. [PubMed: 20522592]
pharmacological research Neuroprotection in non-transgenic and transgenic mouse models of Alzheimer's disease by positive modulation of σ1 receptors [Pubmed: 31048034]
EPILEPSY & BEHAVIOR Fenfluramine acts as a positive modulator of sigma-1 receptors [Pubmed: 32169824]
Journal of Oncology Using Serological Proteome Analysis to Identify and Evaluate Anti-GRP78 Autoantibody as Biomarker in the Detection of Gastric Cancer [33381181]
Heliyon Glucose-regulated protein 78 (GRP78) in renal cell carcinoma: A novel biomarker for predicting tumor behavior []
Medicine (Baltimore) Levels of circulating GRP78 and CHOP in endoplasmic reticulum stress pathways in Chinese type 2 diabetic kidney disease patients [34414940]
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