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乳铁传递蛋白(LTF)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Lactoferrin (LTF)

HLF2; LF; GIG12; Lactotransferrin; Growth-inhibiting protein 12; Talalactoferrin; Lfcin-H; Kaliocin-1

  • 乳铁传递蛋白(LTF)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 乳铁传递蛋白(LTF)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对乳铁传递蛋白(LTF)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

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编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
NPA780Hu01 乳铁传递蛋白(LTF)天然蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
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RPA780Hu01 乳铁传递蛋白(LTF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
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PAA780Hu01 乳铁传递蛋白(LTF)多克隆抗体 WB; IHC; ICC; IP.
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PAA780Hu05 乳铁传递蛋白(LTF)多克隆抗体 WB; IHC; ICC; IP.
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LAA780Hu71 乳铁传递蛋白(LTF)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA780Hu22 乳铁传递蛋白(LTF)单克隆抗体 WB; IHC; ICC; IP.
LAA780Hu72 乳铁传递蛋白(LTF)单克隆抗体(生物素标记) WB; IHC; ICC.
LAA780Hu82 乳铁传递蛋白(LTF)单克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
SEA780Hu 乳铁传递蛋白(LTF)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
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LMA780Hu 乳铁传递蛋白(LTF)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA780Hu01 乳铁传递蛋白(LTF)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
The International Journal of Biochemistry & Cell Biology Changes of hepatic lactoferrin gene expression in two mouse models of the acute phase reaction [ScienceDirect: S1357272511002524]
BMC Veterinary Research Changes in composition of colostrum of Egyptian buffaloes and Holstein cows [BioMed: 17466148]
Plos One p38MAPK, ERK and PI3K Signaling Pathways Are Involved in C5a-Primed Neutrophils for ANCA-Mediated Activation [PlosOne: source]
The Journal of Biological Chemistry Sphingolipid Pathway Regulates Innate Immune Responses at the Fetomaternal Interface during Pregnancy [Pubmed:25505239]
PLoS One Control of Methicillin-Resistant Staphylococcus aureus Pneumonia Utilizing TLR2 Agonist Pam3CSK4 [Pubmed:26974438]
Bioscience, Biotechnology, and Biochemistry Salivary lactoferrin is transferred into the brain via the sublingual route [pubmed:28351211]
Pulmonary Pharmacology & Therapeutics Effects of fluticasone propionate and budesonide on the expression of immune defense genes in bronchial epithelial cells [Pubmed:29627483]
Microbial Pathogenesis TLR2 agonist Pam3CSK4 enhances the antibacterial functions of GM-CSF induced neutrophils to methicillin-resistant Staphylococcus aureus []
Assessment of the Diagnostic Value of IL-10 and Lactoferrin by BIRADS Categories in Breast Neoplasms []
J Clin Med A Three-Protein Panel to Support the Diagnosis of Sepsis in Children [Pubmed:35329889]
Food Funct Three Urban of China: A Cohort Study of Maternal-Infant Factors and HM Protein Components [Pubmed:35438093]
Biomedicines Myeloid-Specific Pyruvate-Kinase-Type-M2-Deficient Mice Are Resistant to Acute Lung Injury [Pubmed:35625931]
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