甘露糖结合蛋白(MBL)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Mannose Binding Lectin (MBL)
MBL2; COLEC1; HSMBPC; MBP1; MBP; Collectin-1; Mannose-binding protein C; Mannan Binding Protein; Mannose-Binding Lectin(Protein C)2,Soluble(Opsonic Defect)
- 编号KSB480Ra01
- 物种Rattus norvegicus (Rat,大鼠) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.312-20ng/mL
- 可适灵敏度0.126ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 7798 ¥ 12996 ¥ 23393 ¥ 45486 ¥ 77976
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特异性
试剂盒中的抗体对甘露糖结合蛋白(MBL)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Rattus norvegicus (Rat,大鼠) | 应用(仅供研究使用,不用于临床诊断!) |
RPB480Ra02 | 甘露糖结合蛋白(MBL)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPB480Ra01 | 甘露糖结合蛋白(MBL)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB480Ra02 | 甘露糖结合蛋白(MBL)多克隆抗体 | WB; IHC; ICC; IP. |
PAB480Ra01 | 甘露糖结合蛋白(MBL)多克隆抗体 | WB; IHC; ICC; IP. |
LAB480Ra71 | 甘露糖结合蛋白(MBL)多克隆抗体(生物素标记) | WB; IHC; ICC. |
SEB480Ra | 甘露糖结合蛋白(MBL)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB480Ra | 甘露糖结合蛋白(MBL)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSB480Ra01 | 甘露糖结合蛋白(MBL)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
American Journal of Nephrology | Analysis of the Urine Proteome of Human Contrast-Induced Kidney Injury Using Two-Dimensional Fluorescence Differential Gel Electrophoresis/Matrix-Assisted Laser Desorption Time-of-Flight Mass Spectrometry/Liquid Chromatography Mass Spectrometry. [Karger: 000255439] |
Current Eye Research | Serum Levels and H/L Gene Polymorphism of Mannose-Binding Lectin in Primary Open Angle Glaucoma [Ingenta: art00007] |
Journal of Clinical Immunology | Complement activation contributes to the injury and outcome of kidney in human anti-glomerular basement membrane disease. [PubMed: 22941511] |
Clinical and Experimental Immunology | Urinary mannose-binding lectin is a biomarker for predicting the progression of immunoglobulin (Ig)A nephropathy [PubMed: PMC3406374] |
Clin J Am Soc Nephrol. | Alternative Complement Pathway Activation Products in Urine and Kidneys of Patients with ANCA-Associated GN [Pubmed: 24115193] |
ACTA TROPICA | Novel findings on the role of ficolins and colectins in the innate response against Leishmania braziliensis [Pubmed: 32827456] |
PLoS One | Complement activation profile of patients with primary focal segmental glomerulosclerosis [Pubmed: 32569286] |
LIFE SCIENCES | The lectin pathway of complement and the initial recognition of Leishmania infantum promastigotes [34242658] |
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