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S100钙结合蛋白A8(S100A8)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for S100 Calcium Binding Protein A8 (S100A8)

MRP8; MIF; 60B8AG; CAGA; CFAG; CGLA; CP-10; L1Ag; NIF; P8; Calgranulin A; Cystic fibrosis antigen; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8

  • S100钙结合蛋白A8(S100A8)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • S100钙结合蛋白A8(S100A8)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对S100钙结合蛋白A8(S100A8)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种：Mus musculus (Mouse，小鼠) 应用(仅供研究使用，不用于临床诊断！)
RPB792Mu02 S100钙结合蛋白A8(S100A8)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPB792Mu61 S100钙结合蛋白A8(S100A8)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB792Mu61 S100钙结合蛋白A8(S100A8)活性蛋白 Cell culture; Activity Assays.
RPB792Mu01 S100钙结合蛋白A8(S100A8)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB792Mu01 S100钙结合蛋白A8(S100A8)多克隆抗体 WB; IHC; ICC; IP.
PAB792Mu02 S100钙结合蛋白A8(S100A8)多克隆抗体 WB; IHC; ICC; IP.
LAB792Mu81 S100钙结合蛋白A8(S100A8)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAB792Mu71 S100钙结合蛋白A8(S100A8)多克隆抗体(生物素标记) WB; IHC; ICC.
MAB792Mu21 S100钙结合蛋白A8(S100A8)单克隆抗体 WB; IHC; ICC; IP.
SEB792Mu S100钙结合蛋白A8(S100A8)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB792Mu S100钙结合蛋白A8(S100A8)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSB792Mu01 S100钙结合蛋白A8(S100A8)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Biochemical and Biophysical Research Communications High circulating levels of S100A8/A9 complex (calprotectin) in male Japanese with abdominal adiposity and dysregulated expression of S100A8 and S100A9 in adipose tissues of obese mice [ScienceDirect: S0006291X1200352X]
American Journal of Reproductive Immunology Association of Increased S100A8 Serum Protein with Early Pregnancy Loss [Pubmed:25252120]
the faseb journal Soluble receptor for advanced glycation end products inhibits disease progression in autosomal dominant polycystic kidney disease by down-regulating cell proliferation [PubMed: 25934702]
J Immunol S100A8 Production in CXCR2-Expressing CD11b+ Gr-1high Cells Aggravates Hepatitis in Mice Fed a High-Fat and High-Cholesterol Diet [PubMed: 26608915]
PLoS One Analytical Performance of ELISA Assays in Urine: One More Bottleneck towards Biomarker Validation and Clinical Implementation [Pubmed:26889680]
Journal of Alzheimer's Disease Proteomics Analysis of Blood Serums from Alzheimer's Disease Patients Using iTRAQ LabelingTechnology. [pubmed:27911324]
 Journal of Functional Foods Standardized ethanolic extract of the rhizome of Curcuma xanthorrhiza prevents murine ulcerative colitis by regulation of inflammation [S1756464617300208]
International Journal of Molecular Medicine Proinflammatory effects of S100A8/A9 via TLR4 and RAGE signaling pathways in BV-2 microglial cells [pubmed:28498464]
International journal of molecular sciences Immune Modulating Topical S100A8/A9 Inhibits Growth of Pseudomonas aeruginosa and Mitigates Biofilm Infection in Chronic Wounds. [pubmed:28672877]
Gastroenterology Tu1938-Micronutrients Assessment in Patients with Cirrhosis [10.1016:S0016-5085(18)33544-3]
Gastroenterology Tu1942-PI3K/AKT-Mediated Upregulation of C7 Inhibits Colorectal Cancer Proliferation and Metastasis [10.1016:S0016-5085(18)33544-5]
Journal of Biological Chemistry Signaling between pancreatic β-cells and macrophages via S100 calcium-binding protein A8 exacerbates β-cell apoptosis and islet inflammation [Pubmed:29496993]
European review for medical and pharmacological sciences S100A8/A9 promotes MMP-9 expression in the fibroblasts from cardiac rupture after myocardial infarction by inducing macrophages secreting TNFα. [Pubmed:29949169]
Foodborne Pathogens and Disease Synergistic effect of immunomodulatory S100A8/A9 and ciprofloxacin against Pseudomonas aeruginosa biofilm in a murine chronic wound model [Pubmed: 31116394]
Kidney International Reports Systemic Inflammation Precedes Microalbuminuria in Diabetes []
Cancer Management and Research iTRAQ-based quantitative protein expression profiling of biomarkers in childhood B-cell and T-cell acute lymphoblastic leukemia [Pubmed: 31440093]
scandinavian journal of clinical & laboratory investigation Serum S100A6, S100A8, S100A9 and S100A11 proteins in colorectal neoplasia: results of a single centre prospective study [Pubmed: 31856598]
ANNALS OF AGRICULTURAL AND ENVIRONMENTAL MEDICINE Analysis of novel serum markers of fibrosis and angiogenesis in patients with alcoholic liver cirrhosis []
MOLECULAR VISION Quantification of a panel for dry-eye protein biomarkers in tears: A comparative pilot study using standard ELISA and customized microarrays [34012227]
university of copenhagen Adjunctive S100A8/A9 Immunomodulation Hinders Cipro fl oxacin Resistance in []
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