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泛素羧基端酯酶L1(UCHL1)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1)

PARK5; PGP9.5; Uch-L1; Neuron cytoplasmic protein 9.5; Ubiquitin thioesterase L1; Ubiquitin carboxyl-terminal hydrolase isozyme L1

  • 泛素羧基端酯酶L1(UCHL1)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 泛素羧基端酯酶L1(UCHL1)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对泛素羧基端酯酶L1(UCHL1)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
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LAG945Ra71 泛素羧基端酯酶L1(UCHL1)多克隆抗体(生物素标记) WB; IHC; ICC.
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MAG945Ra21 泛素羧基端酯酶L1(UCHL1)单克隆抗体 WB; IHC; ICC; IP.
SEG945Ra 泛素羧基端酯酶L1(UCHL1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCG945Ra 泛素羧基端酯酶L1(UCHL1)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMG945Ra 泛素羧基端酯酶L1(UCHL1)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSG945Ra01 泛素羧基端酯酶L1(UCHL1)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
J Neurol Sci Changes of ubiquitin C-terminal hydrolase-L1 levels in serum and urine of patients with white matter lesions [PubMed: 26232084]
Journal of the Neurological Sciences Increased plasma UCH-L1 after aneurysmal subarachnoid hemorrhage is associated with unfavorable neurological outcome [Pubmed:26810533]
Ulutas Medical Journal Serum Carnosine Dipeptidase 1 and Ubiquitin C - Terminal Hydrolase L1 as Markers of Brain Damage in Patients after Carotid Endarterectomy [mnstemps:135]
Scientific Reports Activation of hepatic stellate cells by the ubiquitin C-terminal hydrolase 1 protein secreted from hepatitis C virus-infected hepatocytes [pubmed:28667290]
Intensive Care Medicine Relationships between markers of neurologic and endothelial injury during critical illness and long-term cognitive impairment and disability [Pubmed:29523900]
Earth and Environmental Science Serum concentration of ubiquitin c-terminal hydrolase-L1 in detecting severity of traumatic brain injury [article:10.1088]
Peripheral blood biomarkers in aneurysmal subarachnoid hemorrhage [ISBN:978-952-03-0750-9]
Journal of Critical Care Association of neuronal repair biomarkers with delirium among survivors of critical illness [Pubmed: 31896448]
Brain Sciences BDNF and IL-8, But Not UCHL-1 and IL-11, Are Markers of Brain Injury in Children Caused by Mild Head Trauma [Pubmed: 32987792]
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