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维生素D结合蛋白(DBP)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Vitamin D Binding Protein (DBP)

GC; VDBG; VDBP; Group-Specific Component; Gc-globulin

  • 维生素D结合蛋白(DBP)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 维生素D结合蛋白(DBP)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对维生素D结合蛋白(DBP)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

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参考文献

杂志 参考文献
Proteome Science Isotope Coded Protein Labeling analysis of plasma specimens from acute severe dengue fever patients [PubMed: 23101585]
Toxins (Basel). Increased Circulating Levels of Vitamin D Binding Protein in MS Patients [Pubmed:25590278]
Endocrine Vitamin D-binding protein and free vitamin D concentrations in acromegaly [PubMed: 26547217]
Proteomics Clinical Applications Comparative mass spectrometric and immunoassay‐based proteome analysis in serum of Duchenne muscular dystrophy patients [Pubmed:26680509]
PLoS One. 25-Hydroxivitamin D Serum Concentration, Not Free and Bioavailable Vitamin D, Is Associated with Disease Activity in Systemic Lupus Erythematosus Patients. [pubmed:28085957]
PROTEOMICS - Clinical Applications Comparative mass spectrometric and immunoassay-based proteome analysis in serum ofDuchenne muscular dystrophy patients. [pubmed:26680509]
Plos One 25-Hydroxivitamin D serum concentration, not free and bioavailable vitamin D, is associated with disease activity in systemic lupus erythematosus patients [10.1371/journal.pone.0170323]
Nutrients Gene Expression of Sirtuin-1 and Endogenous Secretory Receptor for Advanced Glycation End Products in Healthy and Slightly Overweight Subjects after … [Pubmed:30037068]
BBA- Molecular Basis of Disease Enhanced remedial effects for vitamin D3 and calcium co-supplementation against pre-existing lead nephrotoxicity in mice: The roles of renal calcium homeostatic … [Pubmed: 30553018]
J Clin Res Pediatr Endocrinol Is Bioavailable Vitamin D Better Than Total Vitamin D to Evaluate Vitamin D Status in Obese Children? [34013709]
PLoS One Leukocyte telomere length as a compensatory mechanism in vitamin D metabolism [Pubmed:35202418]
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