无翅型MMTV整合位点家族成员3A(WNT3A)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Wingless Type MMTV Integration Site Family, Member 3A (WNT3A)
- 编号KSP155Mu01
- 物种Mus musculus (Mouse,小鼠) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.156-10ng/mL
- 可适灵敏度0.054ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 7776 ¥ 12960 ¥ 23328 ¥ 45360 ¥ 77760
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对无翅型MMTV整合位点家族成员3A(WNT3A)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Mus musculus (Mouse,小鼠) | 应用(仅供研究使用,不用于临床诊断!) |
RPP155Mu01 | 无翅型MMTV整合位点家族成员3A(WNT3A)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPP155Mu02 | 无翅型MMTV整合位点家族成员3A(WNT3A)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAP155Mu01 | 无翅型MMTV整合位点家族成员3A(WNT3A)多克隆抗体 | WB; IHC; ICC; IP. |
PAP155Mu02 | 无翅型MMTV整合位点家族成员3A(WNT3A)多克隆抗体 | WB; IHC; ICC; IP. |
SEP155Mu | 无翅型MMTV整合位点家族成员3A(WNT3A)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
HEP155Mu | 无翅型MMTV整合位点家族成员3A(WNT3A)检测试剂盒(酶联免疫吸附试验法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMP155Mu | 无翅型MMTV整合位点家族成员3A(WNT3A)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSP155Mu01 | 无翅型MMTV整合位点家族成员3A(WNT3A)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
Medical Oncology | Wls promotes the proliferation of breast cancer cells via Wnt signaling [Pubmed:25801232] |
J Histochem Cytochem | Wnt3a Mediates the Inhibitory Effect of Hyperoxia on the Transdifferentiation of AECIIs to AECIs [PubMed: 26209081] |
PloS one | Gelatin device for the delivery of growth factors involved in endochondral ossification [pubmed:28380024] |
Life Sciences | Hepatoprotective effect of hesperidin in hepatocellular carcinoma: Involvement of Wnt signaling pathways. [pubmed:28754618] |
Oxidative Medicine and Cellular Longevity | Secreted Frizzled-Related Protein-2 Inhibits Doxorubicin Induced Apoptosis Mediated Through the Akt-mTOR Pathway in Soleus Muscle [Pubmed:30151071] |
The Showa University Journal of Medical Sciences | Eribulin Treatment Induces High Expression of miR-195 and Inactivates the Wnt/β-catenin Signaling Pathway in Triple-negative Breast Cancer [Doi: 10.15369/sujms.30.359] |
Journal of Cancer | Oncogenic Wnt3a: A Candidate Specific Marker and Novel Molecular Target for Hepatocellular Carcinoma [Pubmed: 31737122] |
CANCER LETTERS | Knock down of BMSC-derived Wnt3a or its antagonist analogs attenuate colorectal carcinogenesis induced by chronic Fusobacterium nucleatum infection [Pubmed: 32920199] |
Biomaterials | An improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties [34823885] |
留言咨询