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酸性神经鞘磷脂酶(ASM)活性蛋白

Active Acid Sphingomyelinase (ASM)

SMPD1; NPD; SMase; aSMase; Sphingomyelin Phosphodiesterase 1,Acid Lysosomal; Simply Sphingomyelinase

  • 酸性神经鞘磷脂酶(ASM)活性蛋白 产品包装(模拟)
  • 酸性神经鞘磷脂酶(ASM)活性蛋白 产品包装(模拟)
  • 酸性神经鞘磷脂酶(ASM)活性蛋白 Figure . Gene Sequencing (extract)
  • APB360Mu61.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Sphingomyelin phosphodiesterase 1 (SMPD1), also known as acid sphingomyelinase (ASM), belongs to the sphingomyelin phosphodiesterase family. The protein Converts sphingomyelin to ceramide. ASM also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol.

Thus, the recombinant mouse ASM activity was measured by its ability to hydrolyze 2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine (HDA-PC) to p-Nitrophenol. The reaction was performed in 50mM MES, 0.5 µM ZnCl2, pH 7.5 ( Assay Buffer), initiated by addition 50μL of various concentrations of ASM (diluted with Assay Buffer) to 50 µL of 1mM Substrate HDA-PC ( 50mM stock solution in methanol, diluted with Assay Buffer). Incubated at room temperature for 20 minutes in the dark, then read at a wavelength of 405 nm.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Mus musculus (Mouse，小鼠) 应用(仅供研究使用，不用于临床诊断！)
EPB360Mu61 酸性神经鞘磷脂酶(ASM)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB360Mu61 酸性神经鞘磷脂酶(ASM)活性蛋白 Cell culture; Activity Assays.

参考文献

杂志 参考文献
Blood.? Acid sphingomyelinase is activated in sickle cell erythrocytes and contributes to inflammatory microparticle generation in SCD [Pubmed:25075126]
Journal of Clinical Lipidology Increase in acid sphingomyelinase level in human retinal endothelial cells and CD34+ circulating angiogenic cells isolated from diabetic individuals is associated with dysfunctional retinal vasculature and vascular repair process in diabetes [10.1016/j.jacl.2017.03.007]
Diabetes & metabolism Fenofibrate decreases plasma ceramide in type 2 diabetes patients: A novel marker of CVD? [pubmed:28499696]
INFLAMMATION Acid Sphingomyelinase and Acid ¦Â-Glucosidase 1 Exert Opposite Effects on Interleukin-1¦Â-Induced Interleukin 6 Production in Rheumatoid Arthritis Fibroblast-Like Synoviocytes [33665756]
Indian J Ophthalmol Evaluation of sphingolipid metabolism on diabetic retinopathy [34708809]
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