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转录激活因子3(ATF3)活性蛋白

Active Activating Transcription Factor 3 (ATF3)

Cyclic AMP-dependent Transcription Factor ATF3

  • 转录激活因子3(ATF3)活性蛋白 产品包装(模拟)
  • 转录激活因子3(ATF3)活性蛋白 产品包装(模拟)
  • APC303Mu01.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Activating transcription factor 3 (ATF3) is a stress-induced transcription factor that plays vital roles in modulating metabolism, immunity and oncogenesis. ATF3 acts as a hub of the cellular adaptive-response network. Multiple extracellular signals, such as endoplasmic reticulum (ER) stress, cytokines, chemokines, and LPS, are connected to ATF3 induction. Proto-oncogene c-Fos can bind to ATF3 to regulate signal transduction, cell proliferation and differentiation. Thus a functional ELISA assay was conducted to detect the interaction of recombinant mouse ATF3 and recombinant human FOS. Briefly, ATF3 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to FOS-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ATF3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse ATF3 and recombinant human FOS was shown in Figure 1, the EC50 for this effect is 0.13 ug/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
RPC303Mu02 转录激活因子3(ATF3)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APC303Mu01 转录激活因子3(ATF3)活性蛋白 Cell culture; Activity Assays.
RPC303Mu01 转录激活因子3(ATF3)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAC303Mu02 转录激活因子3(ATF3)多克隆抗体 WB; IHC; ICC; IP.
PAC303Mu01 转录激活因子3(ATF3)多克隆抗体 WB; IHC; ICC; IP.
SEC303Mu 转录激活因子3(ATF3)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC303Mu 转录激活因子3(ATF3)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
PGC303Mu01 转录激活因子3(ATF3)引物对 PCR
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