非组织特异性碱性磷酸酶(ALPL)活性蛋白
Active Alkaline Phosphatase, Tissue-nonspecific (ALPL)
BALP; BSAP; HOPS; AP-TNAP; TNSALP; Bone Alkaline Phosphatase; Alkaline Phosphatase,Tissue-Nonspecific Isozyme; Alkaline Phosphatase, Liver/Bone/Kidney
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产品包装(模拟)
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产品包装(模拟)
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Figure. SDS-PAGE
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通过ISO 9001、ISO 13485质量体系认证
活性实验
Four distinct genes encode alkaline phosphatases (APs) in humans. The ALPL gene encodes the liver/bone/kidney isozyme, also known as the tissue nonspecific AP (TNAP). In comparison, ALPI, ALPP and ALPPL2 encode intestinal, placental and placental-like or germ cell APs, respectively. The serum levels of human APs are useful tumor markers. There are many mutations in the ALPL gene, leading to different forms of hypophosphatasia, characterized by poorly mineralized cartilage and bones. The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. The activity assay of recombinant mouse ALPL was measured by its ability to cleave a peptide substrate, 4-Methumbelliferly phosphate. The reaction was performed in 50 mM Tris, 1 mM MgCl2, pH 9.0 ( assay buffer), ainitiated by addition 50 μl of 0.3 ug/ml ALPL (diluted by assay buffer) to 50 µL of 50 uM substrate. Read at excitation and emission wavelengths of 365 nm and 445 nm (top read), respectively, in kinetic mode for 5 minutes. The specific activity of recombinant mouse ALPL is >11000 pmol/min/µg.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| APB091Mu61 | 非组织特异性碱性磷酸酶(ALPL)活性蛋白 | Cell culture; Activity Assays. |
| RPB091Mu02 | 非组织特异性碱性磷酸酶(ALPL)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPB091Mu61 | 非组织特异性碱性磷酸酶(ALPL)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB091Mu01 | 非组织特异性碱性磷酸酶(ALPL)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB091Mu02 | 非组织特异性碱性磷酸酶(ALPL)多克隆抗体 | WB; IHC; ICC; IP. |
| PAB091Mu01 | 非组织特异性碱性磷酸酶(ALPL)多克隆抗体 | WB; IHC; ICC; IP. |
| LAB091Mu81 | 非组织特异性碱性磷酸酶(ALPL)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAB091Mu71 | 非组织特异性碱性磷酸酶(ALPL)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| SEB091Mu | 非组织特异性碱性磷酸酶(ALPL)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB091Mu | 非组织特异性碱性磷酸酶(ALPL)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Journal of Materials Science: Materials in Medicine | Osteopenic bone cell response to strontium-substituted hydroxyapatite [SpringerLink: n8233337w171v451] |
| Osteoporosis International | Dextromethorphan inhibits osteoclast differentiation by suppressing RANKL-induced nuclear factor-κB activation [PubMed: 23400250] |
| Journal of Applied Toxicology | Chronic exposure to low concentrations of strontium 90 affects bone physiology but not the hematopoietic system in mice [Wiley: Source] |
| Acta Pharmacologica Sinica | The antidepressant bupropion exerts alleviating properties in an ovariectomized osteoporotic rat model [Pubmed:25544359] |
| Biomedical Materials | Enhanced differentiation of osteoblastic cells on novel chitosan/β-1, 3-glucan/bioceramic scaffolds for bone tissue regeneration [Pubmed:25586067] |
| Pharmacology | Effect of Mirtazapine on Rat Bone Tissue after Orchidectomy [PubMed: 25871861] |
| J Biomed Mater Res A. | New method for the fabrication of highly osteoconductive β-1,3-glucan/HA scaffold for bone tissue engineering: Structural, mechanical, and biological characterization. [Pubmed:27239050] |
| J Bone Miner Metab | MiR-142-5p promotes bone repair by maintaining osteoblast activity [Pubmed:27085967] |
| Journal of Biomedical Materials Research | New method for the fabrication of highly osteoconductive β‐1, 3‐glucan/HA scaffold for bone tissue engineering: Structural, mechanical, and biological … [doi:10.1002] |
| Journal of Bone and Mineral Metabolism | MiR‑142‑5p promotes bone repair by maintaining osteoblast activity [pubmed:27085967] |
| Scientific Reports | Hydrolysis of Extracellular Pyrophosphate increases in post-hemodialysis plasma [Pubmed:30038263] |
| International Journal of Molecular Sciences | Suppression Effect of Astaxanthin on Osteoclast Formation In Vitro and Bone Loss In Vivo [Pubmed:29562730] |
| Pharmacology | Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive Rats [Pubmed:29898457] |
| Experimental and Therapeutic Medicine | Combination therapy with BMP‑2 and psoralen enhances fracture healing in ovariectomized mice [10.3892:etm.2018.6353] |
| American Journal of Translational Research | Long non-coding RNA SNHG7 promotes the fracture repair through negative modulation of miR-9 [] |
| The effect of low temperature atmospheric nitrogen plasma on MC3T3-E1 preosteoblast proliferation and differentiation in vitro [] | |
| International Journal of Biomaterials | Bovine Hydroxyapatite-Based Bone Scaffold with Gentamicin Accelerates Vascularization and Remodeling of Bone Defect [34104195] |