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血管紧张素转化酶(ACE)活性蛋白

Active Angiotensin I Converting Enzyme (ACE)

CD143; ACE1; DCP1; ACEI; ACE-I; Kininase II; Angiotensin-Converting Enzyme; Peptidyl-Dipeptidase A; Dipeptidyl Carboxypeptidase 1; Angiotensin-converting enzyme, soluble form

  • 血管紧张素转化酶(ACE)活性蛋白 产品包装(模拟)
  • 血管紧张素转化酶(ACE)活性蛋白 产品包装(模拟)
  • APA004Hu02.png Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Figure. The binding activity of ACE with ACTb.
Angiotensin-converting enzyme ACE, is a central component of the renin-angiotensin system (RAS), which controls blood pressure by regulating the volume of fluids in the body. It converts the hormone angiotensin I to the active vasoconstrictor angiotensin II. Therefore, ACE indirectly increases blood pressure by causing blood vessels to constrict. ACE inhibitors are widely used as pharmaceutical drugs for treatment of cardiovascular diseases. Besides, Actin Beta (ACTb) has been identified as an interactor of ACE, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ACE and recombinant human ACTb. Briefly, ACE were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to ACTb-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ACE pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of ACE and ACTb was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA004Hu01 血管紧张素转化酶(ACE)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA004Hu02 血管紧张素转化酶(ACE)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA004Hu02 血管紧张素转化酶(ACE)活性蛋白 Cell culture; Activity Assays.
PAA004Hu01 血管紧张素转化酶(ACE)多克隆抗体 WB; IHC
PAA004Hu02 血管紧张素转化酶(ACE)多克隆抗体 WB; ICC; IHC-P; IHC-F; ELISA.
LAA004Hu81 血管紧张素转化酶(ACE)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA004Hu71 血管紧张素转化酶(ACE)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA004Hu22 血管紧张素转化酶(ACE)单克隆抗体 WB; IHC; ICC; IP.
MAA004Hu21 血管紧张素转化酶(ACE)单克隆抗体 WB; IHC; ICC; IP.
SEA004Hu 血管紧张素转化酶(ACE)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA004Hu 血管紧张素转化酶(ACE)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA004Hu 血管紧张素转化酶(ACE)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Lipids in Health and disease Association of elevated blood pressure and impaired vasorelaxation in experimental Sprague-Dawley rats fed with heated vegetable oil [PubMed: PMC2914008]
Malaysian Journal of Medical Sciences Effect of Repeatedly Heated Palm Olein on Blood Pressure–Regulating Enzymes Activity and Lipid Peroxidation in Rats [Usm: MJMS19120]
Journal of Cancer Therapy Plasma Levels of Angiotensin-Converting Enzymes 1 and 2 and AGTR2 (T1247G and A5235G) Gene Polymorphisms Are Associated to Breast Cancer Progression. [Scirp: Source]
The American journal of clinical nutrition Vascular function and atherosclerosis progression after 1 y of flavonoid intake in statin-treated postmenopausal women with type 2 diabetes: a double-blind randomized controlled trial. [Pubmed: 23553151]
Journal of Vascular Surgery Cathepsin G deficiency reduces periaortic calcium chloride injury-induced abdominal aortic aneurysms in mice [Pubmed:25037606]
Reprod Toxicol.? ACE2 activation by xanthenone prevents leptin-induced increases in blood pressure and proteinuria during pregnancy in Sprague-Dawley rats [Pubmed:25205467]
Chemical Research in Toxicology Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal Instillation [Pubmed:25580880]
Chem Biol Interact Arsenic causes aortic dysfunction and systemic hypertension in rats: Augmentation of angiotensin II signaling [PubMed: 26079204]
Biomed Res Int Protective Effect of Enalapril against Methionine-Enriched Diet-Induced Hypertension: Role of Endoplasmic Reticulum and Oxidative Stress [PubMed: 26640794]
Clinics (Sao Paulo) Mechanisms [PubMed: 26602523]
Chemical Research in Toxicology Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal Instillation [PubMed: 25580880]
Intensive Care Medicine Experimental Angiotensin converting enzymes in patients with acute respiratory distress syndrome [Content: 3]
Evid Based Complement Alternat Med. Garlic Attenuates Plasma and Kidney ACE-1 and AngII Modulations in Early Streptozotocin-Induced Diabetic Rats: Renal Clearance and Blood Pressure Implications [Pubmed:27293465]
Вавиловский журнал генетики и селекции Ренин-ангиотензин-альдостероновая система у крыс линии НИСАГ (ISIAH) со стресс-индуцированной артериальной гипертензией [30_Dubinina.pdf]
Animal Biology The effect of heat stress on bull sperm quality and related HSPs expression [content:journals]
biomedicine & pharmacotherapy Citrus leaf extract reduces blood pressure and vascular damage in repeatedly heated palm oil diet-Induced hypertensive rats. [pubmed:28068636]
Biomedicine & Pharmacotherapy Parkia speciosa empty pod prevents hypertension and cardiac damage in rats given N(G)-nitro- L -arginine methyl ester [pubmed:28992471]
Journal of Functional Foods Antihypertensive effects of Ocimum gratissimumextract: Angiotensin-converting enzyme inhibitor in vitro and in vivo investigation [10.1016/j.jff.2017.05.033]
Molecules Protective Effect of Polyphenol-Rich Extract from Bee Pollen in a High-Fat Diet [Pubmed:29614743]
Journal of Surgical Research Impact of angiotensin-converting enzyme 2 levels on postoperative pneumonia after esophagectomy [Pubmed:29506841]
Intensive Care Medicine Angiotensin-converting enzymes in acute respiratory distress syndrome []
International Journal of Biological Sciences Vascular Protection of TPE-CA on Hyperhomocysteinemia-induced Vascular Endothelial Dysfunction Through AA Metabolism Modulated CYPs Pathway []
Medicine Alteration and association between serum ACE2/angiotensin (1-7)/Mas axis and oxidative stress in chronic kidney disease: A pilot study [Pubmed: 32756181]
American Journal of Respiratory and Critical Care Medicine Equilibrium Angiotensin Metabolite Profiling in Patients with Acute Respiratory Distress Syndrome Indicates Angiotensin Converting Enzyme Inhibition [Pubmed: 32628511]
Int Immunopharmacol Crocin protects against cardiotoxicity induced by doxorubicin through TLR-2/NF-κB signal pathway in vivo and vitro [Pubmed: 32388215]
Food Bioscience Djulis (Chenopodium formosanum) and its bioactive compounds affect vasodilation, angiotensin converting enzyme activity, and hypertension []
Journal of Extracellular Vesicles MiR155-5p in adventitial fibroblasts-derived extracellular vesicles inhibits vascular smooth muscle cell proliferation via suppressing angiotensin-converting enzyme … []
Biotechnology and Pharmacy Chrysin pretreatment improves mitochondrial enzymes and angiotensin converting enzymes in L-NAME induced hypertensive rats []
Dikkat Eksikliği Hiperaktivite Bozukluğu Tanısı Olan Anjiyotensin I, II, III, Alamandin peptid, Anjiyotensin Dönüştürücü Enzim (ACE), NADPH Oksidaz Enzim Serum … []
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