B-淋巴细胞激活抗原B7-1(LAB7-1)活性蛋白
Active B-Lymphocyte Activation Antigen B7-1 (LAB7-1)
CD80; CD28LG; CD28LG1; BB1; B7-1 Antigen; T-lymphocyte activation antigen CD80; CTLA-4 counter-receptor B7.1
- 编号APB386Ra01
- 物种Rattus norvegicus (Rat，大鼠) 相同的名称，不同的物种。
- 缓冲液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 90%
- 等电点5.0
- 应用Cell culture; Activity Assays.
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- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1344 ¥ 3360 ¥ 6720 ¥ 20160 ¥ 50400
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产品包装(模拟)
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产品包装(模拟)
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Figure. SDS-PAGE
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通过ISO 9001、ISO 13485质量体系认证
活性实验
B-Lymphocyte Activation Antigen B7-1 (CD80) is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. A functional binding ELISA assay was conducted to detect the interaction of recombinant rat CD80 with recombinant human IL10. Briefly, recombinant rat CD80 were diluted serially in PBS, with 0.01% BSA (pH7.4). Duplicate samples of 100 ul were then transferred to IL10-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CD80 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant rat CD80 and recombinant human IL10 was shown in Figure 1, the EC50 for this effect is 0.09 ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| APB386Ra01 | B-淋巴细胞激活抗原B7-1(LAB7-1)活性蛋白 | Cell culture; Activity Assays. |
| RPB386Ra01 | B-淋巴细胞激活抗原B7-1(LAB7-1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
参考文献
| 杂志 | 参考文献 |
| BioMed Research International | Predictability of Urinary CD80 in the Relapse of Primary Nephrotic Syndrome [pubmed:28951877] |