骨成型蛋白受体2(BMPR2)活性蛋白
Active Bone Morphogenetic Protein Receptor 2 (BMPR2)
BMPRII; BMPR-II; BRK3; T-ALK; BMPR3; PPH1; Primary Pulmonary Hypertension 1; BMP type II receptor
- 编号APA016Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 90%
- 等电点4.9
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1262 ¥ 3156 ¥ 6312 ¥ 18936 ¥ 47340
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产品包装(模拟)
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产品包装(模拟)
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Figure. SDS-PAGE
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通过ISO 9001、ISO 13485质量体系认证
活性实验
Bone Morphogenetic Protein Receptor 2 (BMPR2) is a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. BMPR2 is a TGFβ type II receptor expressed on cumulus cells. Growth Differentiation Factor 9 (GDF9) can bind to BMPR2 through different type I receptors, thereby regulating downstream metabolic reactions. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human BMPR2 and recombinant human GDF9. Briefly, biotin-linked BMPR2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to GDF9-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µl stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human BMPR2 and recombinant human GDF9 was shown in Figure 1, the EC50 for this effect is 42.87 ng/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA016Hu01 | 骨成型蛋白受体2(BMPR2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA016Hu01 | 骨成型蛋白受体2(BMPR2)活性蛋白 | Cell culture; Activity Assays. |
| PAA016Hu01 | 骨成型蛋白受体2(BMPR2)多克隆抗体 | WB; ICC; IHC-P; IHC-F; ELISA. |
| SEA016Hu | 骨成型蛋白受体2(BMPR2)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA016Hu | 骨成型蛋白受体2(BMPR2)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Biomedicines | Participation of Selected Soluble BMP-2 and BMP-7 Bone Morphogenetic Proteins and Their Soluble Type IALK-1 and Type IIBMPR2 Receptors in Formation and … [34680408] |