钙蛋白酶2(CAPN2)活性蛋白
Active Calpain 2 (CAPN2)
CANPL2; CANPml; mCANP; Calpain-2 Catalytic Subunit; Calcium-activated neutral proteinase 2; Calpain M-type; Calpain large polypeptide L2; Millimolar-calpain; Calpain 2, Large Subunit
- 编号APC358Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 95%
- 等电点5.0
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 暂无报价 暂无报价 暂无报价 暂无报价 暂无报价
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产品包装(模拟)
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产品包装(模拟)
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Figure. SDS-PAGE
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通过ISO 9001、ISO 13485质量体系认证
活性实验
Calpain 2 (CAPN2) is a calcium-regulated, non-lysosomal thiolprotease that is known for its crucial role in catalyzing the limited proteolysis of substrates essential for cytoskeletal remodeling and signaling pathways. It belongs to the family of calpains, a group of enzymes that are activated by calcium ions.Besides,CAPN1 has been identified as an interactor of CAPN2, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human CAPN2 and recombinant rat CAPN1. Briefly, CAPN2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CAPN1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CAPN2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant humanCAPN2 and recombinant rat CAPN1 was shown in Figure 1, the EC50 for this effect is 0.044ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPC358Hu01 | 钙蛋白酶2(CAPN2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APC358Hu01 | 钙蛋白酶2(CAPN2)活性蛋白 | Cell culture; Activity Assays. |
| PAC358Hu01 | 钙蛋白酶2(CAPN2)多克隆抗体 | WB; IHC; ICC/IF |
| MAC358Hu21 | 钙蛋白酶2(CAPN2)单克隆抗体 | WB; IHC; ICC; IP. |
| SEC358Hu | 钙蛋白酶2(CAPN2)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC358Hu | 钙蛋白酶2(CAPN2)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |